Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GU3_RS15795 Genome accession   NC_016745
Coordinates   3360577..3362289 (+) Length   570 a.a.
NCBI ID   WP_014293534.1    Uniprot ID   -
Organism   Oceanimonas sp. GK1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3355577..3367289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GU3_RS15770 (GU3_15350) pdhR 3356649..3357410 (-) 762 WP_014293529.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  GU3_RS15775 (GU3_15355) ampD 3357806..3358354 (-) 549 WP_014293530.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GU3_RS15780 (GU3_15360) - 3358427..3358945 (+) 519 WP_014293531.1 TIGR02281 family clan AA aspartic protease -
  GU3_RS15785 (GU3_15365) nadC 3358995..3359849 (+) 855 WP_014293532.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GU3_RS16935 (GU3_15370) - 3360138..3360575 (+) 438 WP_014293533.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GU3_RS15795 (GU3_15375) pilB 3360577..3362289 (+) 1713 WP_014293534.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GU3_RS15800 (GU3_15380) pilC 3362315..3363541 (+) 1227 WP_014293535.1 type II secretion system F family protein Machinery gene
  GU3_RS15805 (GU3_15385) pilD 3363892..3364746 (+) 855 WP_014293536.1 A24 family peptidase Machinery gene
  GU3_RS15810 (GU3_15390) coaE 3364743..3365360 (+) 618 WP_014293537.1 dephospho-CoA kinase -
  GU3_RS15815 (GU3_15395) trmB 3365492..3366208 (-) 717 WP_014293538.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63091.48 Da        Isoelectric Point: 5.2987

>NTDB_id=43282 GU3_RS15795 WP_014293534.1 3360577..3362289(+) (pilB) [Oceanimonas sp. GK1]
MTIPINTTRSGLARSLAAAGLLSSAELDQVQDLARQEGKPLSTMLVDRQLLSSPELARFCEQEYGQPLLDLDDFDLEQLP
PQFLSMDLIERHHAIPVKLDNRVLYLAMSDPTHVAALEDFGFRFNLVTDTLLVEEDKLQQLLLRLQQSGGTGLDLDGIAD
DDIAGLELDQDPSEREDQFDHNEDDAPIVRYINKIMLDAVRKEASDLHFEPFERFFRIRFRIDGMLHEVASPPVHLASRF
AARLKVMARLDIAERRLPQDGRIKLKLTRSRSVDMRVNTLPTMWGEKVVIRLLDSSAARLDIDQLGYSEEQKALYLHALR
RPQGMILVTGPTGSGKTVSLYTGLSILNTVTANIATAEDPVEINLPGINQVQINPKAGLSFAHALRAFLRQDPDIIMVGE
IRDLETAEISVKAAQTGHLVLSTLHTNSAAETLTRLGNMGLPAYNIASSVSLIMAQRLARKLCLHCRTPELLPTEQLTRL
GFTPAQLADGMTLYRAVGCAHCTEGYKGRIGVYEVMAMSERLAQLIMENAGSLALARAAEEEGMVPLRQAALDKARRGII
SLAEVNRITG

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=43282 GU3_RS15795 WP_014293534.1 3360577..3362289(+) (pilB) [Oceanimonas sp. GK1]
ATGACCATTCCCATTAATACCACCCGCAGCGGCCTGGCCCGCAGCCTGGCCGCCGCCGGGCTGCTGTCTTCTGCCGAGCT
GGACCAGGTGCAGGACCTCGCCCGGCAGGAAGGCAAGCCGCTCAGCACCATGCTGGTGGACAGGCAGTTACTCAGCTCCC
CCGAGCTGGCCCGTTTCTGCGAGCAGGAATACGGCCAGCCGCTGCTGGATCTCGACGACTTCGACCTCGAACAGCTGCCG
CCCCAGTTTCTGAGCATGGATCTGATCGAGCGCCATCATGCCATTCCGGTGAAGCTGGACAACCGGGTGCTCTACCTGGC
CATGTCGGATCCCACCCATGTGGCGGCATTGGAAGACTTCGGCTTTCGCTTCAACCTGGTCACCGACACCCTGCTGGTGG
AAGAAGACAAACTGCAGCAACTGCTGCTCAGGTTGCAGCAAAGCGGCGGCACCGGGCTGGATCTTGACGGTATTGCCGAC
GACGACATCGCCGGCCTGGAGCTGGACCAGGATCCGTCGGAGCGGGAAGACCAGTTCGACCACAACGAGGACGATGCCCC
CATCGTCCGTTATATCAACAAGATCATGCTGGACGCGGTGCGCAAGGAGGCCTCGGATCTGCATTTCGAGCCCTTTGAGC
GCTTTTTCCGCATTCGCTTTCGCATCGACGGCATGCTGCACGAGGTGGCCTCACCGCCGGTGCACCTGGCGTCCCGCTTC
GCCGCCCGACTCAAGGTGATGGCCCGGCTCGACATCGCCGAGCGCCGACTGCCCCAGGACGGCCGCATCAAGCTCAAGCT
GACCCGCTCCAGGTCGGTGGACATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAGAAAGTGGTCATTCGTCTTCTCG
ACAGCTCCGCCGCCCGTCTCGATATCGATCAGCTGGGCTACAGCGAGGAGCAAAAGGCGCTGTACCTGCATGCCCTGAGG
CGCCCCCAGGGCATGATACTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTGTCGCTCTATACCGGCCTGAGCATTCT
CAATACCGTCACCGCCAATATCGCCACCGCCGAAGACCCGGTGGAAATCAACCTGCCCGGCATCAACCAGGTGCAGATCA
ATCCCAAGGCCGGGCTGTCTTTTGCCCACGCCCTGCGCGCCTTTCTGCGCCAGGATCCGGACATCATCATGGTGGGGGAA
ATCCGCGATCTGGAAACCGCCGAAATTTCGGTCAAGGCGGCCCAGACCGGCCACCTGGTGCTGTCGACCCTGCACACCAA
CTCCGCCGCCGAGACCCTTACCCGCCTTGGTAACATGGGACTGCCGGCCTACAACATCGCCTCGTCGGTGAGTCTTATCA
TGGCCCAGCGGCTGGCGCGCAAGCTGTGCCTGCACTGCCGCACTCCTGAGCTGCTGCCCACCGAGCAGCTGACCCGGCTT
GGCTTTACCCCCGCCCAGCTGGCCGACGGCATGACCCTGTACCGGGCGGTGGGGTGTGCCCATTGCACCGAGGGCTACAA
GGGCCGTATCGGCGTCTATGAGGTCATGGCCATGAGCGAGCGGCTGGCCCAGCTCATTATGGAAAACGCCGGCTCCCTGG
CCCTGGCCCGGGCGGCGGAAGAGGAAGGCATGGTCCCCCTGCGCCAGGCCGCCCTGGACAAGGCGCGACGCGGCATCATC
AGCCTGGCGGAGGTCAACCGCATCACCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

52.827

99.298

0.525
  pilB Vibrio cholerae strain A1552

52.128

98.947

0.516
  pilB Acinetobacter baylyi ADP1

50.622

98.772

0.5
  pilB Acinetobacter baumannii D1279779

50.355

98.947

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

50.177

99.298

0.498
  pilB Vibrio campbellii strain DS40M4

50.266

98.772

0.496
  pilF Neisseria gonorrhoeae MS11

49.156

93.509

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

37.702

100

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.676

100

0.389

Multiple sequence alignment