Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GU3_RS15805 Genome accession   NC_016745
Coordinates   3363892..3364746 (+) Length   284 a.a.
NCBI ID   WP_014293536.1    Uniprot ID   H2FV12
Organism   Oceanimonas sp. GK1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3358892..3369746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GU3_RS15785 (GU3_15365) nadC 3358995..3359849 (+) 855 WP_014293532.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GU3_RS16935 (GU3_15370) - 3360138..3360575 (+) 438 WP_014293533.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GU3_RS15795 (GU3_15375) pilB 3360577..3362289 (+) 1713 WP_014293534.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GU3_RS15800 (GU3_15380) pilC 3362315..3363541 (+) 1227 WP_014293535.1 type II secretion system F family protein Machinery gene
  GU3_RS15805 (GU3_15385) pilD 3363892..3364746 (+) 855 WP_014293536.1 A24 family peptidase Machinery gene
  GU3_RS15810 (GU3_15390) coaE 3364743..3365360 (+) 618 WP_014293537.1 dephospho-CoA kinase -
  GU3_RS15815 (GU3_15395) trmB 3365492..3366208 (-) 717 WP_014293538.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  GU3_RS15820 (GU3_15400) lysS 3366392..3367879 (-) 1488 WP_014293539.1 lysine--tRNA ligase -

Sequence

Protein


Download         Length: 284 a.a.        Molecular weight: 31491.77 Da        Isoelectric Point: 8.0846

>NTDB_id=43284 GU3_RS15805 WP_014293536.1 3363892..3364746(+) (pilD) [Oceanimonas sp. GK1]
MTELLDNTALLYMVIPLLGLLVGSFLNVVIHRLPLMMQRQWQRECAGLNNQPVADEAPFNLCVPRSRCPHCQHVLTVKDN
IPLLSWLWLKGRCRYCRAPIAKRYPLVELASALIAALALWRFGPGPQLLAALVFSWLLLCMTLIDLDHLLLPDDLTLPLL
WLGLLLNVGELFVSLPSAVIGAAAGYGVLWSLYWLFKLATGKEGMGYGDFKLLAAIGAWFGWQSLLPVLLLASFTGAALG
LGLMALRRLGEDRVLPFGPALALGGWCYLMWGTEVVNLYWRLAL

Nucleotide


Download         Length: 855 bp        

>NTDB_id=43284 GU3_RS15805 WP_014293536.1 3363892..3364746(+) (pilD) [Oceanimonas sp. GK1]
ATGACCGAACTGCTCGACAATACCGCTCTGCTTTATATGGTGATTCCCCTGCTCGGCCTGCTGGTGGGCAGTTTTCTCAA
TGTGGTGATCCACCGGCTGCCGTTGATGATGCAGCGGCAGTGGCAACGGGAATGCGCCGGACTGAACAACCAGCCGGTGG
CCGACGAGGCCCCCTTCAACCTGTGCGTCCCCCGCTCCCGCTGCCCCCACTGCCAGCATGTGCTGACCGTCAAAGACAAT
ATACCCCTGCTCAGCTGGCTATGGCTGAAAGGCCGCTGCCGCTACTGCCGCGCGCCCATCGCCAAACGTTACCCCCTGGT
GGAGCTGGCTTCGGCGCTGATTGCCGCCCTTGCGCTGTGGCGCTTTGGTCCCGGCCCCCAGTTGCTGGCGGCATTGGTGT
TCAGCTGGCTGCTGCTGTGCATGACCCTGATCGATCTGGATCACCTGCTGCTGCCCGACGATCTGACCCTACCGCTGCTG
TGGCTGGGGCTGCTGCTGAATGTGGGCGAACTGTTTGTCTCCCTGCCCTCGGCGGTGATCGGTGCCGCCGCCGGTTACGG
CGTGTTGTGGAGCCTGTACTGGCTGTTTAAACTGGCCACCGGCAAGGAAGGCATGGGCTATGGCGACTTCAAGCTGCTCG
CCGCCATCGGCGCCTGGTTTGGCTGGCAAAGCCTGCTGCCGGTGCTGCTGCTGGCGTCCTTTACCGGCGCCGCCCTGGGG
CTGGGGCTGATGGCCCTGCGCCGCCTGGGGGAAGACAGAGTCCTGCCCTTTGGCCCGGCCCTGGCGCTGGGGGGCTGGTG
CTACCTGATGTGGGGCACCGAGGTGGTGAATCTTTACTGGAGGCTGGCGTTATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-122)

Type IV leader peptidase family (Peptidase_A24)

(132-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H2FV12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

54.682

94.014

0.514
  pilD Vibrio campbellii strain DS40M4

50.186

94.718

0.475
  pilD Acinetobacter nosocomialis M2

45.324

97.887

0.444
  pilD Acinetobacter baumannii D1279779

45.324

97.887

0.444
  pilD Neisseria gonorrhoeae MS11

46.825

88.732

0.415

Multiple sequence alignment