Detailed information NTG0432676

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HB663_RS14555
• Genome accession: NZ_CP050432
• Coordinates: 3014049..3015167 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347035.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain PM194229
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3009049..3020167

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HB663_RS14545 (HB663_14545)	-	3012165..3012857 (-)	693	WP_001108510.1	YggS family pyridoxal phosphate-dependent enzyme	-
HB663_RS14550 (HB663_14550)	pilT	3012984..3014021 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
HB663_RS14555 (HB663_14555)	pilU	3014049..3015167 (+)	1119	WP_000347035.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HB663_RS14560 (HB663_14560)	fur	3015237..3015674 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
HB663_RS14565 (HB663_14565)	-	3015786..3016184 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
HB663_RS14570 (HB663_14570)	-	3016239..3016562 (-)	324	WP_000442585.1	RnfH family protein	-
HB663_RS14575 (HB663_14575)	-	3016562..3017629 (-)	1068	WP_005120426.1	hypothetical protein	-
HB663_RS14580 (HB663_14580)	-	3017782..3018237 (-)	456	WP_000782976.1	bacteriohemerythrin	-
HB663_RS14585 (HB663_14585)	-	3018490..3019338 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41821.98 Da
• Isoelectric Point: 6.5675

>NTDB_id=432676 HB663_RS14555 WP_000347035.1 3014049..3015167(+) (pilU) [Acinetobacter baumannii strain PM194229]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDSQ

Nucleotide

• Length: 1119 bp

>NTDB_id=432676 HB663_RS14555 WP_000347035.1 3014049..3015167(+) (pilU) [Acinetobacter baumannii strain PM194229]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGCGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACAGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.817	100	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39