Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DSUI_RS06425 Genome accession   NC_016616
Coordinates   1378521..1380239 (-) Length   572 a.a.
NCBI ID   WP_014236421.1    Uniprot ID   G8QME2
Organism   Azospira oryzae PS     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1373521..1385239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSUI_RS06400 (Dsui_1315) aspS 1373574..1375373 (-) 1800 WP_014236416.1 aspartate--tRNA ligase -
  DSUI_RS06405 (Dsui_1316) - 1375404..1376045 (-) 642 WP_014236417.1 DUF502 domain-containing protein -
  DSUI_RS06410 (Dsui_1317) - 1376024..1376296 (-) 273 WP_014236418.1 FmdB family zinc ribbon protein -
  DSUI_RS06415 (Dsui_1318) pilD 1376395..1377249 (-) 855 WP_014236419.1 prepilin peptidase Machinery gene
  DSUI_RS06420 (Dsui_1319) pilC 1377252..1378496 (-) 1245 WP_014236420.1 type II secretion system F family protein Machinery gene
  DSUI_RS06425 (Dsui_1320) pilB 1378521..1380239 (-) 1719 WP_014236421.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DSUI_RS06430 (Dsui_1321) - 1380515..1381354 (-) 840 WP_014236422.1 cytochrome C assembly family protein -
  DSUI_RS06435 (Dsui_1322) ffh 1381400..1382755 (+) 1356 WP_014236423.1 signal recognition particle protein -
  DSUI_RS06440 (Dsui_1323) - 1383073..1383681 (-) 609 WP_014236424.1 lytic transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62241.46 Da        Isoelectric Point: 5.3501

>NTDB_id=43133 DSUI_RS06425 WP_014236421.1 1378521..1380239(-) (pilB) [Azospira oryzae PS]
MAATFQVPLSGLARAMVQAGRLKEAEAEAFMAQASEKGTTFIEQVIAGKRASAKEIAVFAGETFGYPVLDLSAFDEANIP
ASAIDRKLIATHRVIPLNKRGNRLSVAIADPTNLRALDEIRFQTGLAVDSVVVEEPKLAPLVVKYSESAEENLKNLTNED
LNLEFAEEEDQAKADEAVSLDVDDAPVVKFIQKILLDAINDGASDIHFEPYEKFYRIRYRIDGELREVANPPLAIKEKVA
SRIKVISRMDISEKRVPQDGRMKLVLSKTRAIDFRVSTLPTLHGEKIVMRILDPSSATLGIEALGYEPEQKEALLNAIHR
PYGMVLVTGPTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLAGINQVNVNDKAGLTFSAALKSFLRQDPDIIMVGEI
RDLDTAEIAIKAAQTGHMVLSTLHTNDAPTTLERLKNMGVPTFNIASSVILITAQRLARRLCNCKVPLHVPTETLLNAGF
TEAELDGSWTLYGPNPEGCERCKGGGFKGRVGIYQVMPVSEEIQRIIMANGNSMDIAAQAKKEGVKDLRQSGLLKVKQGV
TSLDEVLSTTNA

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=43133 DSUI_RS06425 WP_014236421.1 1378521..1380239(-) (pilB) [Azospira oryzae PS]
ATGGCAGCTACTTTTCAGGTTCCCCTAAGTGGTCTTGCGCGCGCCATGGTCCAGGCTGGACGCCTCAAAGAGGCCGAAGC
CGAAGCCTTCATGGCCCAAGCCAGCGAAAAGGGCACGACATTCATTGAGCAGGTGATCGCCGGCAAGCGGGCCAGCGCCA
AGGAAATAGCCGTATTCGCAGGAGAAACGTTCGGCTACCCGGTCCTTGATCTGAGCGCTTTCGATGAAGCCAACATTCCG
GCCAGCGCCATTGACCGGAAGCTCATCGCCACCCACCGCGTCATACCGCTGAACAAGCGCGGCAACCGGCTCTCGGTAGC
GATAGCTGACCCAACAAATTTGCGGGCCTTGGACGAAATTCGCTTCCAGACAGGCTTGGCAGTGGACTCCGTCGTTGTCG
AAGAGCCCAAACTGGCACCGCTGGTCGTCAAATATTCGGAATCGGCAGAAGAAAACCTCAAGAATCTCACCAATGAGGAC
TTGAACCTCGAATTTGCGGAAGAAGAAGATCAGGCCAAGGCCGATGAAGCCGTTTCCCTGGATGTGGATGACGCACCGGT
CGTCAAGTTCATTCAGAAAATACTGCTGGATGCGATCAACGATGGCGCTTCCGACATCCACTTCGAGCCGTATGAAAAGT
TTTACCGCATCCGCTATCGCATCGACGGCGAGTTGCGAGAGGTTGCCAATCCGCCCCTGGCAATCAAGGAAAAGGTGGCT
TCCCGCATCAAGGTCATTTCCCGGATGGACATTTCCGAGAAGCGGGTCCCGCAAGACGGCCGGATGAAGCTGGTCCTTTC
CAAAACCAGAGCCATCGACTTCCGGGTCAGCACGCTACCAACGCTGCATGGCGAAAAGATCGTCATGCGTATTCTGGATC
CGTCCTCCGCCACGCTGGGCATCGAAGCGCTGGGCTACGAGCCGGAACAGAAGGAAGCCCTGCTCAACGCCATCCACCGT
CCTTATGGCATGGTTCTGGTAACCGGCCCGACCGGCTCCGGTAAAACCGTATCCTTGTACACCTGCCTCAACATCCTGAA
CAAGCCTGGCATCAACATCTCCACCGCGGAAGACCCGGCAGAAATCAACCTGGCCGGCATCAACCAGGTAAACGTCAACG
ACAAGGCAGGTCTGACCTTTTCCGCCGCCCTCAAGTCCTTCCTGCGGCAGGATCCGGACATCATCATGGTGGGTGAAATC
CGCGATCTGGACACGGCCGAAATCGCAATCAAGGCGGCCCAGACCGGCCACATGGTGCTATCCACCCTGCACACCAACGA
TGCCCCGACGACACTGGAGCGCCTGAAGAACATGGGCGTCCCCACCTTCAACATTGCCTCGTCCGTCATCCTGATCACCG
CCCAGCGCCTGGCCCGCCGCCTGTGCAACTGCAAGGTCCCGCTCCACGTCCCCACCGAAACCCTTCTGAATGCAGGCTTT
ACCGAAGCAGAACTGGATGGCAGTTGGACACTCTATGGCCCCAACCCGGAAGGATGCGAGCGCTGCAAGGGAGGCGGTTT
CAAGGGCCGGGTAGGCATCTACCAGGTGATGCCGGTTTCCGAGGAAATTCAGCGCATCATCATGGCCAATGGCAACTCCA
TGGATATTGCTGCCCAGGCCAAAAAGGAAGGGGTCAAGGACTTGCGTCAGTCCGGCCTGTTGAAAGTAAAGCAGGGAGTC
ACCTCCCTGGACGAAGTGCTGTCTACGACAAACGCCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-151)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G8QME2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.401

100

0.556
  pilB Acinetobacter baylyi ADP1

54.939

100

0.554
  pilB Legionella pneumophila strain ERS1305867

53.322

100

0.533
  pilF Neisseria gonorrhoeae MS11

53.086

99.126

0.526
  pilB Vibrio cholerae strain A1552

49.558

98.776

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

47.687

98.252

0.469
  pilB Vibrio campbellii strain DS40M4

47.08

98.776

0.465
  pilF Thermus thermophilus HB27

38.489

99.476

0.383

Multiple sequence alignment