Detailed information NTG0043131

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: DSUI_RS06415
• Genome accession: NC_016616
• Coordinates: 1376395..1377249 (-)
• Length: 284 a.a.
• NCBI ID: WP_014236419.1
• Uniprot ID: G8QME0
• Organism: Azospira oryzae PS
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1371395..1382249

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DSUI_RS06380 (Dsui_1311)	-	1371739..1372083 (-)	345	WP_014236412.1	hypothetical protein	-
DSUI_RS06385 (Dsui_1312)	nudB	1372252..1372719 (-)	468	WP_014236413.1	dihydroneopterin triphosphate diphosphatase	-
DSUI_RS17560 (Dsui_1313)	-	1372716..1373162 (-)	447	WP_014236414.1	barstar family protein	-
DSUI_RS06395 (Dsui_1314)	-	1373166..1373558 (-)	393	WP_014236415.1	ribonuclease domain-containing protein	-
DSUI_RS06400 (Dsui_1315)	aspS	1373574..1375373 (-)	1800	WP_014236416.1	aspartate--tRNA ligase	-
DSUI_RS06405 (Dsui_1316)	-	1375404..1376045 (-)	642	WP_014236417.1	DUF502 domain-containing protein	-
DSUI_RS06410 (Dsui_1317)	-	1376024..1376296 (-)	273	WP_014236418.1	FmdB family zinc ribbon protein	-
DSUI_RS06415 (Dsui_1318)	pilD	1376395..1377249 (-)	855	WP_014236419.1	prepilin peptidase	Machinery gene
DSUI_RS06420 (Dsui_1319)	pilC	1377252..1378496 (-)	1245	WP_014236420.1	type II secretion system F family protein	Machinery gene
DSUI_RS06425 (Dsui_1320)	pilB	1378521..1380239 (-)	1719	WP_014236421.1	type IV-A pilus assembly ATPase PilB	Machinery gene
DSUI_RS06430 (Dsui_1321)	-	1380515..1381354 (-)	840	WP_014236422.1	cytochrome C assembly family protein	-

Sequence

Protein

• Length: 284 a.a.
• Molecular weight: 30772.58 Da
• Isoelectric Point: 7.6890

>NTDB_id=43131 DSUI_RS06415 WP_014236419.1 1376395..1377249(-) (pilD) [Azospira oryzae PS]
MEFLLLESPALASAVAGILGLLVGSFLNVVIHRLPRMMERDWHLQCAELRGETAPEKERLSLAAPASRCPHCGHAIRAWE
NIPVLSFLLLKGRCSGCQAPISLRYPLVEAFTGLLSAFTVWHFGPTLAAAAALLLLWAMVALTGIDFDTQLLPDSITLPL
LWLGLLFNISGTFTDISSAVIGAMVGYLSLWSVYWLFKLATGKEGMGYGDFKLLAAIGAWLGWQMLPLTILLSSLVGAVV
GVALIVLARRGRNVPIPFGPYLAAAGLLALYWGQELTQSYLRLL

Nucleotide

• Length: 855 bp

>NTDB_id=43131 DSUI_RS06415 WP_014236419.1 1376395..1377249(-) (pilD) [Azospira oryzae PS]
ATGGAATTTTTACTACTGGAAAGTCCCGCCCTTGCCTCTGCTGTGGCCGGAATCCTGGGCCTCCTGGTCGGCAGCTTCCT
CAACGTCGTCATCCACCGCCTGCCACGGATGATGGAGCGGGACTGGCACCTGCAGTGCGCCGAACTGCGGGGGGAAACGG
CTCCGGAAAAGGAACGGCTGAGCCTCGCCGCCCCGGCCTCCCGTTGCCCCCATTGCGGCCACGCCATCCGGGCCTGGGAG
AACATCCCGGTCCTCAGCTTCCTGCTCCTCAAAGGCCGCTGTTCCGGTTGCCAGGCCCCGATTTCCCTGCGCTACCCGTT
GGTGGAAGCCTTCACGGGCCTGCTCTCCGCCTTCACGGTGTGGCACTTCGGCCCGACCCTGGCAGCAGCTGCCGCCCTGT
TGCTGCTGTGGGCCATGGTGGCCCTGACCGGCATCGACTTCGACACCCAGCTGCTGCCGGACTCCATCACCCTGCCCCTG
CTCTGGCTCGGCCTGCTGTTCAACATCTCCGGCACCTTCACCGACATCAGCTCCGCCGTCATCGGCGCCATGGTCGGCTA
CCTGTCCCTGTGGAGCGTCTACTGGCTGTTCAAGCTGGCCACCGGCAAGGAAGGCATGGGCTACGGCGACTTCAAGCTGC
TGGCCGCCATCGGCGCCTGGCTCGGCTGGCAGATGCTGCCCCTGACCATCCTGCTCTCCTCCCTGGTCGGCGCCGTCGTC
GGCGTGGCCCTGATCGTGCTGGCCCGCAGGGGCCGCAACGTGCCCATTCCCTTCGGTCCCTATCTGGCTGCTGCGGGCCT
GCTGGCCCTGTACTGGGGCCAGGAACTGACCCAGTCCTACCTGCGGCTGCTCTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (133-242)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-123)

3D structure

• AlphaFold DB: G8QME0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.986	97.183	0.525
pilD	Acinetobacter baumannii D1279779	54.015	96.479	0.521
pilD	Acinetobacter nosocomialis M2	53.65	96.479	0.518
pilD	Vibrio campbellii strain DS40M4	52.899	97.183	0.514
pilD	Neisseria gonorrhoeae MS11	49.627	94.366	0.468