Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HBA18_RS02340 Genome accession   NZ_CP050266
Coordinates   463329..465011 (-) Length   560 a.a.
NCBI ID   WP_167313972.1    Uniprot ID   -
Organism   Salinivibrio costicola strain M318     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 458329..470011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBA18_RS02310 (HBA18_02310) mutT 459156..459551 (+) 396 WP_069586607.1 8-oxo-dGTP diphosphatase MutT -
  HBA18_RS02315 (HBA18_02315) yacG 459582..459788 (-) 207 WP_069586608.1 DNA gyrase inhibitor YacG -
  HBA18_RS02320 (HBA18_02320) zapD 459793..460533 (-) 741 WP_167313968.1 cell division protein ZapD -
  HBA18_RS02325 (HBA18_02325) coaE 460557..461171 (-) 615 WP_167313969.1 dephospho-CoA kinase -
  HBA18_RS02330 (HBA18_02330) pilD 461171..462040 (-) 870 WP_167313970.1 A24 family peptidase Machinery gene
  HBA18_RS02335 (HBA18_02335) pilC 462105..463319 (-) 1215 WP_167313971.1 type II secretion system F family protein Machinery gene
  HBA18_RS02340 (HBA18_02340) pilB 463329..465011 (-) 1683 WP_167313972.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HBA18_RS02345 (HBA18_02345) - 465082..465516 (-) 435 WP_167313973.1 pilin -
  HBA18_RS02350 (HBA18_02350) nadC 465765..466673 (-) 909 WP_167313974.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HBA18_RS02355 (HBA18_02355) - 466836..467828 (+) 993 WP_167313975.1 ABC transporter substrate-binding protein -
  HBA18_RS02360 (HBA18_02360) - 467825..469129 (+) 1305 WP_167313976.1 diguanylate cyclase -
  HBA18_RS02365 (HBA18_02365) ampD 469157..469711 (+) 555 WP_167313977.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 61836.13 Da        Isoelectric Point: 5.7543

>NTDB_id=430992 HBA18_RS02340 WP_167313972.1 463329..465011(-) (pilB) [Salinivibrio costicola strain M318]
MILTAKLYEAGLLTDAQRDMLGIGNNTSLSLAHILDAGVDPTALANTLATLYHLPFAESHQYPFKDTCYALDRRDLIQRH
QVLPLAQQGQELLLGVSDPDNAEAVADFRFSTGLNVRLVVLSQPDLDAALRHLYGADIDGTGDGITISDQDLAALTVTDS
PQEDSAELQSDQAPISRYIQQILVDAVRKKASDVHFEPFEHHYQIRFRQDGLLQTYAHPPAGVSRRLSTRLKILAQLNIA
ERRLPQDGRIKLTLTADHTVDMRVSTLPTIWGEKVVVRLLDNGQQQLTLHDLGLTEWQRQHYQDALDRPQGMILFTGPTG
SGKTVSLYSGLKYLNQPERNIATVEDPVEIQLEGVNQVNVNTAIGLDFATALRAFLRQDPDVVMVGEIRDLDTAEIGIKA
AQTGHLVLSTLHTNSAAESITRLSHMGIAPYNLASSLTLVVAQRLVRRLCPMCKQPHPADDPHLQHLANLYPTLTPNTLY
QAHPHGCYQCYQGYQGRTGVYEVLPVTETIASAIYRNASAIELTQLATEEGMWRLAESGIAKLNQGITSIQELTRVIQLA

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=430992 HBA18_RS02340 WP_167313972.1 463329..465011(-) (pilB) [Salinivibrio costicola strain M318]
ATGATCCTAACCGCTAAGTTGTATGAAGCCGGTTTATTGACTGATGCCCAGCGCGATATGCTGGGCATTGGCAACAATAC
CTCGCTCAGTCTTGCGCATATACTCGATGCTGGGGTTGACCCTACCGCGCTCGCCAACACCCTCGCCACGCTGTACCATC
TTCCGTTTGCAGAGAGCCATCAATATCCGTTTAAAGACACCTGCTATGCCCTCGACCGGCGTGATTTAATTCAGCGTCAC
CAAGTGCTACCGCTTGCTCAGCAAGGCCAAGAGCTATTACTGGGGGTCAGTGATCCAGATAACGCAGAAGCTGTCGCCGA
CTTTCGCTTTTCTACTGGGCTCAATGTCCGGTTAGTGGTGCTCAGTCAGCCAGATTTGGATGCTGCGTTGCGCCACTTAT
ACGGGGCAGATATCGATGGCACTGGCGATGGTATCACCATCAGTGATCAGGACTTGGCGGCCCTGACCGTCACCGATAGC
CCACAAGAGGACAGTGCCGAGCTACAAAGCGATCAGGCACCCATCAGTCGTTATATTCAACAGATCTTAGTGGATGCGGT
GCGCAAAAAAGCCTCCGACGTGCATTTCGAGCCCTTTGAGCATCACTACCAAATTCGCTTTCGCCAAGACGGTTTATTAC
AAACCTATGCTCATCCTCCAGCAGGGGTCTCGCGGCGACTGTCGACCCGGCTCAAAATCTTAGCCCAACTCAATATTGCC
GAACGACGTTTGCCACAGGACGGGCGCATCAAGCTGACCCTCACCGCCGACCATACCGTCGATATGCGCGTCTCTACCCT
GCCAACCATTTGGGGCGAGAAAGTAGTAGTTCGTCTGTTGGATAATGGCCAGCAGCAATTAACCTTGCATGATTTAGGCT
TAACCGAGTGGCAACGCCAGCACTATCAAGACGCATTGGATAGACCGCAAGGCATGATTTTGTTTACCGGCCCAACCGGC
AGCGGCAAAACCGTCTCTTTATACAGTGGGTTAAAATATCTCAATCAGCCCGAGCGTAATATTGCTACTGTCGAAGATCC
GGTTGAGATCCAGCTAGAAGGCGTCAACCAAGTCAACGTTAATACGGCCATTGGTTTAGATTTTGCCACCGCACTCCGCG
CATTTTTGCGCCAAGATCCCGATGTAGTCATGGTGGGGGAAATTCGCGATCTCGACACCGCTGAAATCGGGATCAAGGCC
GCGCAAACCGGTCATCTGGTGTTATCAACCCTGCATACCAATTCCGCCGCCGAAAGCATCACCCGTTTGTCCCATATGGG
CATTGCCCCCTACAACCTTGCCAGCTCTCTAACTTTGGTGGTGGCCCAACGTTTAGTCCGCCGTTTATGCCCGATGTGTA
AACAGCCGCACCCAGCTGATGATCCGCACCTTCAGCACTTAGCTAACCTCTATCCGACGTTAACCCCAAACACCTTGTAC
CAAGCTCATCCTCACGGCTGCTATCAGTGTTATCAAGGCTATCAGGGTCGCACAGGGGTGTATGAGGTGCTTCCTGTCAC
CGAGACTATTGCCAGCGCCATTTACCGCAATGCCAGTGCCATCGAACTGACCCAACTGGCAACGGAGGAAGGGATGTGGC
GATTGGCCGAATCCGGGATTGCCAAACTCAATCAAGGGATCACCAGCATTCAAGAGCTTACTCGCGTTATTCAGCTTGCC
TAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(9-136)

Type II/IV secretion system protein (T2SSE)

(181-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

53.571

100

0.536
  pilB Vibrio campbellii strain DS40M4

52.857

100

0.529
  pilB Vibrio cholerae strain A1552

53.861

92.5

0.498
  pilB Legionella pneumophila strain ERS1305867

46.923

92.857

0.436
  pilB Acinetobacter baumannii D1279779

45.817

93.929

0.43
  pilB Acinetobacter baylyi ADP1

45.769

92.857

0.425
  pilF Neisseria gonorrhoeae MS11

42.095

93.75

0.395
  pilF Thermus thermophilus HB27

38.857

93.75

0.364