Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   HBA48_RS03245 Genome accession   NZ_CP050135
Coordinates   616532..617800 (+) Length   422 a.a.
NCBI ID   WP_003790915.1    Uniprot ID   -
Organism   Kingella kingae strain ATCC 23331     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 611532..622800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBA48_RS03220 (HBA48_03240) pgeF 611879..612658 (-) 780 WP_243763643.1 peptidoglycan editing factor PgeF -
  HBA48_RS03225 (HBA48_03245) cyaY 612808..613131 (-) 324 WP_099046165.1 iron donor protein CyaY -
  HBA48_RS03230 (HBA48_03250) - 613222..613416 (+) 195 WP_003790918.1 lipoprotein -
  HBA48_RS03235 (HBA48_03255) lysA 613385..614614 (+) 1230 WP_134793864.1 diaminopimelate decarboxylase -
  HBA48_RS03240 (HBA48_03260) pilF 614800..616485 (+) 1686 WP_003787777.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HBA48_RS03245 (HBA48_03265) pilG 616532..617800 (+) 1269 WP_003790915.1 type II secretion system F family protein Machinery gene
  HBA48_RS03250 (HBA48_03270) pilD 617800..618711 (+) 912 WP_080571747.1 A24 family peptidase Machinery gene
  HBA48_RS03255 (HBA48_03275) coaE 618713..619321 (+) 609 WP_003787784.1 dephospho-CoA kinase -
  HBA48_RS03260 (HBA48_03280) zapD 619436..620197 (+) 762 WP_003790912.1 cell division protein ZapD -
  HBA48_RS03265 (HBA48_03285) pncC 620217..620693 (+) 477 WP_003787789.1 nicotinamide-nucleotide amidase -
  HBA48_RS03270 (HBA48_03290) - 620703..621323 (+) 621 WP_003790910.1 L-threonylcarbamoyladenylate synthase -
  HBA48_RS03275 (HBA48_03295) - 621413..622606 (-) 1194 WP_166503195.1 acetate kinase -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46003.46 Da        Isoelectric Point: 10.1438

>NTDB_id=430258 HBA48_RS03245 WP_003790915.1 616532..617800(+) (pilG) [Kingella kingae strain ATCC 23331]
MAAKKPVKKAPVKKAAAAKPVKKEKGNRYQFEGRNLSTDSIVRGEVVAKNEDEARQKLARRQIKVLQLTKMKKAKDKKIT
SADIAVFTRQLSTMMKAGLPLMQAFDIVAKGHSNASMTAMLMEIRNDVEQGEALGDSFAKHPKYFDKFYCNLVAAGEAGG
VLEGLLDKLATYKEKTEAIKKKVKSALTYPISIVVVAVVLVIVMMMFVLPEFGKVYESMGAKLPPLTQFMMDISNAFVQF
GWLIIAGLIGGVVALIQFHKRSPKFQKRVDAMLLKLPVFGDIVRKATIARWARTTATLFTAGVPLVEVLDSVAGAAGNII
YEEATREIRSKVNQGISLTSGMQATNLFPNMVVQMASIGEESGALDDMLNKTAEFYEDEVDAAVAMLSSLMEPIIMVVLG
GIIGTILVAMYLPLFNLGNVVG

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=430258 HBA48_RS03245 WP_003790915.1 616532..617800(+) (pilG) [Kingella kingae strain ATCC 23331]
ATGGCTGCAAAAAAACCTGTTAAAAAAGCACCTGTAAAAAAAGCAGCGGCTGCCAAGCCTGTTAAAAAAGAAAAAGGCAA
TCGCTACCAGTTTGAAGGTCGCAATTTAAGTACCGATTCAATCGTACGCGGCGAAGTGGTTGCCAAAAATGAAGACGAAG
CACGTCAGAAGTTAGCGCGTCGCCAAATTAAGGTGTTGCAACTGACCAAAATGAAAAAGGCAAAAGATAAGAAAATTACT
TCTGCCGATATTGCGGTGTTTACACGCCAGCTTTCTACCATGATGAAAGCAGGCTTGCCATTAATGCAAGCGTTTGACAT
CGTGGCAAAAGGTCATTCCAATGCATCTATGACAGCCATGCTGATGGAAATTCGCAACGATGTGGAACAAGGTGAAGCAT
TGGGCGATTCGTTTGCCAAACACCCTAAATATTTTGACAAATTCTATTGCAACTTGGTTGCCGCAGGTGAAGCAGGCGGT
GTATTGGAAGGCTTGTTAGACAAACTGGCAACCTATAAAGAAAAAACCGAAGCCATTAAGAAAAAAGTGAAATCGGCTTT
GACTTACCCAATTTCTATTGTGGTAGTGGCAGTCGTTTTGGTAATTGTGATGATGATGTTTGTATTGCCTGAGTTTGGTA
AGGTGTATGAAAGCATGGGGGCAAAATTGCCACCTCTCACACAGTTTATGATGGATATTTCTAATGCATTTGTTCAATTT
GGTTGGTTGATTATTGCAGGCTTGATTGGTGGAGTGGTTGCATTGATTCAATTTCATAAAAGATCACCTAAGTTCCAAAA
AAGAGTAGATGCTATGTTGTTAAAATTACCTGTATTTGGTGATATTGTCCGTAAAGCCACGATTGCACGTTGGGCACGTA
CAACCGCCACTTTGTTTACAGCGGGTGTACCATTGGTGGAAGTATTGGATTCGGTAGCAGGTGCCGCGGGTAATATTATT
TATGAAGAGGCCACTCGTGAAATTCGTTCCAAAGTAAACCAAGGTATTTCTTTGACATCGGGTATGCAGGCAACCAATTT
GTTCCCAAACATGGTAGTGCAAATGGCTTCCATTGGTGAAGAATCTGGCGCGTTGGACGATATGTTAAACAAAACTGCCG
AATTCTATGAAGACGAAGTCGATGCTGCGGTAGCCATGCTGTCGTCTTTGATGGAACCCATCATTATGGTGGTATTGGGT
GGCATTATTGGTACGATTCTGGTTGCGATGTACTTACCATTGTTCAACTTGGGTAACGTGGTAGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

68.25

94.787

0.647
  pilG Neisseria meningitidis 44/76-A

67.5

94.787

0.64
  pilC Legionella pneumophila strain ERS1305867

48.995

94.313

0.462
  pilC Acinetobacter baylyi ADP1

45.337

91.469

0.415
  pilC Pseudomonas stutzeri DSM 10701

50.435

81.754

0.412
  pilC Acinetobacter baumannii D1279779

45.195

91.232

0.412
  pilC Vibrio cholerae strain A1552

37.26

98.578

0.367