Detailed information NTG0042961

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: BDGL_000160
• Genome accession: NC_016603
• Coordinates: 166500..167618 (-)
• Length: 372 a.a.
• NCBI ID: YP_004994428.1
• Uniprot ID: F0KFM6
• Organism: Acinetobacter pittii PHEA-2
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 161500..172618

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BDGL_000154	-	162328..163176 (+)	849	YP_004994422.1	putative glutamine amidotransferase	-
BDGL_000155	-	163428..163883 (+)	456	YP_004994423.1	hypothetical protein	-
BDGL_000156	-	164088..165104 (+)	1017	YP_004994424.1	hypothetical protein	-
BDGL_000157	-	165104..165418 (+)	315	YP_004994425.1	hypothetical protein	-
BDGL_000158	omlA	165483..165881 (-)	399	YP_004994426.1	outer membrane lipoprotein	-
BDGL_000159	fur	165993..166430 (+)	438	YP_004994427.1	ferric uptake regulator	-
BDGL_000160	pilU	166500..167618 (-)	1119	YP_004994428.1	twitching motility protein	Machinery gene
BDGL_000161	pilT	167638..168705 (-)	1068	YP_004994429.1	twitching motility protein	Machinery gene
BDGL_000162	yggS	168802..169494 (+)	693	YP_004994430.1	hypothetical protein	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41931.15 Da
• Isoelectric Point: 6.6404

>NTDB_id=42961 BDGL_000160 YP_004994428.1 166500..167618(-) (pilU) [Acinetobacter pittii PHEA-2]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=42961 BDGL_000160 YP_004994428.1 166500..167618(-) (pilU) [Acinetobacter pittii PHEA-2]
ATGGATTTTAATGACCTGCTCAACCTCATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGACAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGGCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTATTACGGCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAATTACCGCCTGTGCTTAAAGATTTATCAATGACCAAACGCGGTATCATTATTTTTGTAGGTGCAACGG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCCATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTCGA
AATTGCCTTAAAGAATACGTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACAGGTCACCTTGTATTAGCCACAATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAGAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCCCCATTAATTTCAGACT
ATATTCGTAAAGGTGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

3D structure

• AlphaFold DB: F0KFM6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.733	99.731	0.685
pilU	Vibrio cholerae strain A1552	53.784	99.462	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363