Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   G8D99_RS13855 Genome accession   NZ_CP049916
Coordinates   3041691..3042227 (-) Length   178 a.a.
NCBI ID   WP_166326883.1    Uniprot ID   A0A6G8S729
Organism   Acinetobacter lanii strain 185     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3015918..3041609 3041691..3042227 flank 82


Gene organization within MGE regions


Location: 3015918..3042227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8D99_RS13715 (G8D99_13715) - 3015918..3016740 (+) 823 WP_166326846.1 IS5 family transposase -
  G8D99_RS13720 (G8D99_13720) - 3017179..3017769 (+) 591 Protein_2655 IS982 family transposase -
  G8D99_RS13725 (G8D99_13725) - 3017783..3018301 (-) 519 WP_166326848.1 IS630 family transposase -
  G8D99_RS13730 (G8D99_13730) - 3018325..3018702 (-) 378 WP_166326851.1 IS630 transposase-related protein -
  G8D99_RS13735 (G8D99_13735) - 3018754..3019044 (+) 291 Protein_2658 transposase -
  G8D99_RS13740 (G8D99_13740) - 3019120..3020219 (-) 1100 Protein_2659 IS3 family transposase -
  G8D99_RS13745 (G8D99_13745) tnpA 3020265..3020693 (+) 429 WP_004783683.1 IS66-like element accessory protein TnpA -
  G8D99_RS13750 (G8D99_13750) tnpB 3020690..3021025 (+) 336 WP_004783682.1 IS66 family insertion sequence element accessory protein TnpB -
  G8D99_RS13755 (G8D99_13755) tnpC 3021100..3022705 (+) 1606 Protein_2662 IS66 family transposase -
  G8D99_RS13760 (G8D99_13760) - 3022736..3022828 (-) 93 Protein_2663 transposase -
  G8D99_RS13765 (G8D99_13765) - 3022977..3023447 (-) 471 WP_166326853.1 transposase family protein -
  G8D99_RS13775 (G8D99_13775) - 3025109..3025567 (-) 459 WP_166326855.1 type IV pilin protein -
  G8D99_RS13780 (G8D99_13780) - 3025561..3026025 (-) 465 WP_166326857.1 type IV pilin protein -
  G8D99_RS13785 (G8D99_13785) - 3026035..3029910 (-) 3876 WP_227554331.1 pilus assembly protein -
  G8D99_RS13790 (G8D99_13790) - 3029922..3030686 (-) 765 WP_171522767.1 pilus assembly protein PilX -
  G8D99_RS13795 (G8D99_13795) - 3030683..3031756 (-) 1074 WP_166326859.1 PilW family protein -
  G8D99_RS13800 (G8D99_13800) pilV 3031759..3032253 (-) 495 WP_166326861.1 type IV pilus modification protein PilV -
  G8D99_RS13805 (G8D99_13805) - 3032247..3032684 (-) 438 WP_166326863.1 Tfp pilus assembly protein FimT/FimU -
  G8D99_RS13810 (G8D99_13810) ispH 3033135..3034085 (-) 951 WP_166326865.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  G8D99_RS13815 (G8D99_13815) gmk 3034218..3034838 (+) 621 WP_166326867.1 guanylate kinase -
  G8D99_RS13820 (G8D99_13820) rpoZ 3034914..3035195 (+) 282 WP_166326869.1 DNA-directed RNA polymerase subunit omega -
  G8D99_RS13825 (G8D99_13825) - 3035398..3037503 (+) 2106 WP_166326871.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  G8D99_RS13830 (G8D99_13830) - 3037581..3037964 (+) 384 WP_166326873.1 RidA family protein -
  G8D99_RS13835 (G8D99_13835) - 3038185..3038379 (+) 195 WP_166326875.1 bacterioferritin-associated ferredoxin -
  G8D99_RS13840 (G8D99_13840) bfr 3038654..3039118 (+) 465 WP_166326877.1 bacterioferritin -
  G8D99_RS13845 (G8D99_13845) - 3039189..3040784 (-) 1596 WP_320108666.1 Wzy polymerase domain-containing protein -
  G8D99_RS13850 (G8D99_13850) tfpZ 3040893..3041609 (-) 717 WP_166326881.1 TfpX/TfpZ family type IV pilin accessory protein -
  G8D99_RS13855 (G8D99_13855) pilE 3041691..3042227 (-) 537 WP_166326883.1 pilin Machinery gene

Sequence

Protein


Download         Length: 178 a.a.        Molecular weight: 17878.31 Da        Isoelectric Point: 4.6282

>NTDB_id=428648 G8D99_RS13855 WP_166326883.1 3041691..3042227(-) (pilE) [Acinetobacter lanii strain 185]
MNAQKGFTLIELMIVVAIIGILAAVAIPAYQNYTVRAKVTELVTAGSAAKAEISEGFQSGGIVGMNGAVGSINAATVASK
YVESVKATADTGVITVTSSEDGSLPTEAQKKTINLVPYITTGGEDGATAQLTATTVSDGESLQWACASAGTATAVGRGLT
ANSVVGTMPAKYVPSECK

Nucleotide


Download         Length: 537 bp        

>NTDB_id=428648 G8D99_RS13855 WP_166326883.1 3041691..3042227(-) (pilE) [Acinetobacter lanii strain 185]
ATGAACGCTCAAAAGGGTTTTACATTAATTGAGTTAATGATCGTTGTTGCGATTATTGGTATTTTGGCTGCAGTTGCGAT
TCCTGCGTATCAAAACTATACAGTGCGTGCAAAAGTCACTGAACTTGTTACAGCTGGTTCTGCAGCAAAAGCTGAAATTA
GCGAAGGTTTCCAAAGCGGTGGAATCGTTGGCATGAATGGGGCTGTTGGCAGCATCAATGCTGCTACAGTAGCAAGTAAA
TATGTAGAAAGTGTAAAAGCTACAGCAGACACAGGTGTAATTACAGTAACCTCTTCTGAAGATGGTAGCTTACCGACTGA
GGCTCAAAAGAAAACAATTAACTTGGTTCCTTATATTACAACTGGCGGTGAAGATGGCGCTACAGCACAGTTAACAGCTA
CTACTGTAAGCGATGGCGAGTCGCTTCAATGGGCATGTGCTTCTGCTGGAACAGCAACTGCAGTAGGTCGTGGTTTAACT
GCTAATAGTGTTGTTGGTACTATGCCAGCGAAATATGTTCCATCTGAATGTAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8S729

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

47.423

100

0.517
  pilA Ralstonia pseudosolanacearum GMI1000

42.222

100

0.427
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.853

99.438

0.376
  pilA2 Legionella pneumophila strain ERS1305867

37.5

98.876

0.371
  pilA2 Legionella pneumophila str. Paris

37.5

98.876

0.371
  pilE Neisseria gonorrhoeae strain FA1090

36.517

100

0.365
  comP Acinetobacter baylyi ADP1

35.519

100

0.365