Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G8E00_RS14455 Genome accession   NZ_CP049801
Coordinates   3180223..3181935 (+) Length   570 a.a.
NCBI ID   WP_166225722.1    Uniprot ID   A0A6G8RZ28
Organism   Acinetobacter shaoyimingii strain 323-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3175223..3186935
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8E00_RS14415 (G8E00_14410) nusA 3175810..3177294 (-) 1485 WP_166011212.1 transcription termination factor NusA -
  G8E00_RS14420 (G8E00_14415) rimP 3177335..3177859 (-) 525 WP_166011214.1 ribosome maturation factor RimP -
  G8E00_RS14445 (G8E00_14440) secG 3178827..3179156 (-) 330 WP_166225718.1 preprotein translocase subunit SecG -
  G8E00_RS14450 (G8E00_14445) tpiA 3179170..3179961 (-) 792 WP_166011218.1 triose-phosphate isomerase -
  G8E00_RS14455 (G8E00_14450) pilB 3180223..3181935 (+) 1713 WP_166225722.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G8E00_RS14460 (G8E00_14455) pilC 3181962..3183194 (+) 1233 WP_166225725.1 type II secretion system F family protein Machinery gene
  G8E00_RS14465 (G8E00_14460) pilD 3183229..3184089 (+) 861 WP_166225728.1 A24 family peptidase Machinery gene
  G8E00_RS14470 (G8E00_14465) coaE 3184117..3184728 (+) 612 WP_166225731.1 dephospho-CoA kinase -
  G8E00_RS14475 (G8E00_14470) - 3184725..3185645 (-) 921 WP_166011229.1 DMT family transporter -
  G8E00_RS14480 (G8E00_14475) rlmB 3185711..3186457 (-) 747 WP_166011231.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  G8E00_RS14485 (G8E00_14480) - 3186590..3186913 (-) 324 WP_166011233.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63257.81 Da        Isoelectric Point: 5.0199

>NTDB_id=427919 G8E00_RS14455 WP_166225722.1 3180223..3181935(+) (pilB) [Acinetobacter shaoyimingii strain 323-1]
MTLLQSGIKFSGYNRRLVDEGILTADQMQSAIESTKKAKTKMVPHLVEQLNIKPLTLAQTIAQEFGEPLFDLDAYNTDLV
PKEVVESSLVNKHHILPLNIRGSTLYVAMCDPIDMEGLEAIRLNSKLKVVPVVVEYNKLVKMIDKIFAESDNFDFGSEDL
DVDVVDEDATNNDKNDSEPSTDDSPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIANPPLQMANRLAS
RLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNALPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKALFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKAFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCTHCKRPADVPKESLIEMGF
TEEDLASPDFQMFEPVGCPECREGYKGRVGIYEVMRITPEISKIIMQDGNSLEIAEASQKAGFNDLRRSGLVKVMSGLTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=427919 G8E00_RS14455 WP_166225722.1 3180223..3181935(+) (pilB) [Acinetobacter shaoyimingii strain 323-1]
ATGACCTTACTACAAAGTGGTATTAAATTTTCGGGGTACAATCGACGTCTCGTCGATGAAGGTATTTTAACAGCTGATCA
AATGCAATCGGCCATTGAAAGTACCAAAAAAGCTAAAACTAAAATGGTCCCGCATTTGGTTGAACAGCTCAATATCAAAC
CATTAACACTTGCACAAACGATTGCGCAGGAGTTTGGGGAACCTCTATTTGATCTAGATGCCTACAATACGGACTTAGTT
CCAAAAGAAGTTGTCGAAAGTAGCTTGGTCAATAAACACCACATTTTACCTTTGAATATTCGTGGTAGCACCCTCTATGT
CGCAATGTGTGATCCAATTGACATGGAAGGTCTTGAAGCCATTCGCCTCAACAGTAAACTCAAAGTCGTTCCAGTTGTTG
TTGAATACAACAAATTGGTCAAAATGATTGATAAGATTTTCGCTGAATCAGATAATTTTGATTTCGGTAGCGAAGATTTA
GACGTTGATGTTGTTGATGAAGATGCTACTAACAATGATAAAAATGATTCAGAGCCATCGACCGATGACTCCCCTATCGT
TAAATACATTAATAAATTATTGGTTGATGCGATTCGCATGGGGGCTTCAGATTTGCACTTTGAACCCTATGAAAAATCAT
ACCGTGTACGTTACCGTGTCGACGGTGTTCTAAGACATATTGCCAATCCACCACTACAAATGGCGAATCGTCTTGCTTCT
CGTTTAAAAGTCATGTCACAAATGGACATTTCAGAAAAACGTATGCCACAAGATGGTCGTATTAAATTAAAGCTTTCTAA
AACAAAAGCCATCGACTTTCGTGTCAATGCCTTACCAACGCTGTTTGGTGAAAAGCTGGTACTGCGTATTTTAGATCCGT
CAAGTGCCATGTTGGGTATTGATGCTTTAGGTTATGAACCAGAACAAAAAGCATTATTTATGGAGGCGCTAGAAAAACCT
CAAGGGATGTTACTCATTACGGGTCCTACAGGTTCAGGTAAAACCGTGTCTCTGTATACTGGCTTGAATATTCTCAACAC
TGAAGACACCAATATTTCAACTGCGGAAGACCCTGTAGAGATTAACTTGGAAGGGATTAATCAGGTCAACGTAAACCCCA
AAGTAGGCTTAACCTTCTCTGCCGCGCTAAAAGCATTCCTGCGTCAAGATCCAGATGTGATTATGGTCGGGGAGATTCGT
GATTTAGAAACGGCTGAAATTGCGATTAAGGCAGCCCAAACAGGTCATATGGTGATGTCGACGCTACATACCAACAGCGC
ACCTGAAACATTAACGCGTCTCCGCAATATGGGCGTTCCTTCATTCAACATCGCAACTTCAGTCAATTTAGTCATTGCAC
AACGTCTGGCACGTCGTTTATGTACACATTGTAAAAGACCTGCCGATGTACCCAAAGAAAGTTTGATTGAGATGGGCTTT
ACTGAAGAAGATTTGGCCAGCCCAGATTTTCAAATGTTTGAGCCTGTAGGTTGTCCAGAATGTCGGGAAGGATATAAAGG
ACGTGTGGGGATTTATGAGGTGATGCGGATTACACCTGAAATTTCAAAAATTATTATGCAAGACGGTAATTCCTTAGAAA
TTGCTGAAGCATCACAGAAAGCAGGTTTTAATGATTTACGTCGTTCAGGATTAGTCAAAGTGATGAGCGGCTTAACTTCT
CTACAGGAAGTGAATCGTGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8RZ28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

75.439

100

0.754
  pilB Acinetobacter baylyi ADP1

72.105

100

0.721
  pilB Legionella pneumophila strain ERS1305867

51.034

100

0.519
  pilF Neisseria gonorrhoeae MS11

50.178

98.596

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

48.75

98.246

0.479
  pilB Vibrio cholerae strain A1552

47.312

97.895

0.463
  pilB Vibrio campbellii strain DS40M4

47.217

97.719

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384