Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   G8E00_RS14465 Genome accession   NZ_CP049801
Coordinates   3183229..3184089 (+) Length   286 a.a.
NCBI ID   WP_166225728.1    Uniprot ID   A0A6G8RYX8
Organism   Acinetobacter shaoyimingii strain 323-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3178229..3189089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8E00_RS14445 (G8E00_14440) secG 3178827..3179156 (-) 330 WP_166225718.1 preprotein translocase subunit SecG -
  G8E00_RS14450 (G8E00_14445) tpiA 3179170..3179961 (-) 792 WP_166011218.1 triose-phosphate isomerase -
  G8E00_RS14455 (G8E00_14450) pilB 3180223..3181935 (+) 1713 WP_166225722.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G8E00_RS14460 (G8E00_14455) pilC 3181962..3183194 (+) 1233 WP_166225725.1 type II secretion system F family protein Machinery gene
  G8E00_RS14465 (G8E00_14460) pilD 3183229..3184089 (+) 861 WP_166225728.1 A24 family peptidase Machinery gene
  G8E00_RS14470 (G8E00_14465) coaE 3184117..3184728 (+) 612 WP_166225731.1 dephospho-CoA kinase -
  G8E00_RS14475 (G8E00_14470) - 3184725..3185645 (-) 921 WP_166011229.1 DMT family transporter -
  G8E00_RS14480 (G8E00_14475) rlmB 3185711..3186457 (-) 747 WP_166011231.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  G8E00_RS14485 (G8E00_14480) - 3186590..3186913 (-) 324 WP_166011233.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32129.58 Da        Isoelectric Point: 7.9981

>NTDB_id=427921 G8E00_RS14465 WP_166225728.1 3183229..3184089(+) (pilD) [Acinetobacter shaoyimingii strain 323-1]
MHELTALFIEYPIYLYITVGIISLCIGSFLNVVIYRTPKMMEQEWKQDCQMLLHPEQAIIDESKITLSKPDSSCPKCKQP
IRWYQNIPVISWLALRGKCGSCKNPISIRYPLIELLTAICSLIVVWVYGPTLQAFFGVILTWVLIALTFIDFDTQLLPDR
YTLPLAALGLGINSYAIYTTPTSAIWGYIIGFLCLWIVYYLFKIITGKEGMGYGDFKLLAALGAWMGPMLLPLIILLSSC
VGAMIGIILLKVKKENQPFAFGPYIAIAGWIAFLWGDQIMKAYLGG

Nucleotide


Download         Length: 861 bp        

>NTDB_id=427921 G8E00_RS14465 WP_166225728.1 3183229..3184089(+) (pilD) [Acinetobacter shaoyimingii strain 323-1]
ATGCATGAACTAACTGCTTTATTTATTGAATACCCAATATATCTCTATATTACAGTCGGTATTATCAGCCTATGTATCGG
CAGTTTTCTCAATGTCGTGATTTATCGTACGCCAAAAATGATGGAACAAGAGTGGAAACAAGACTGCCAAATGCTCTTGC
ATCCAGAACAAGCCATTATTGATGAATCTAAAATTACATTGAGCAAACCAGACTCAAGCTGTCCCAAATGCAAGCAACCG
ATTCGTTGGTATCAGAATATTCCAGTAATCAGTTGGCTTGCTTTACGTGGTAAATGCGGTTCATGCAAAAATCCGATTAG
CATTCGTTATCCTTTGATCGAACTGCTAACTGCTATTTGTTCTTTAATTGTGGTTTGGGTTTACGGTCCTACCTTACAGG
CATTTTTTGGTGTCATTTTGACTTGGGTTCTCATCGCATTAACCTTTATCGATTTTGACACTCAACTCCTGCCAGACCGT
TATACCTTACCCTTAGCAGCACTGGGTTTAGGCATCAATAGCTATGCTATCTATACCACGCCTACATCAGCTATTTGGGG
CTATATTATTGGTTTCTTATGTCTTTGGATTGTCTATTACCTGTTTAAAATCATCACTGGTAAAGAAGGTATGGGCTATG
GAGATTTCAAACTCTTAGCGGCATTGGGTGCTTGGATGGGACCCATGCTGTTGCCATTGATCATCTTGCTATCTTCTTGT
GTTGGTGCAATGATTGGCATCATCCTGCTCAAGGTTAAAAAAGAAAACCAACCTTTTGCTTTTGGTCCATATATTGCCAT
TGCAGGATGGATTGCTTTTCTATGGGGCGATCAAATCATGAAAGCCTATTTAGGAGGCTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)

Type IV leader peptidase family (Peptidase_A24)

(139-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8RYX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

78.322

100

0.783
  pilD Acinetobacter nosocomialis M2

78.322

100

0.783
  pilD Vibrio cholerae strain A1552

52.482

98.601

0.517
  pilD Vibrio campbellii strain DS40M4

51.943

98.951

0.514
  pilD Neisseria gonorrhoeae MS11

48.669

91.958

0.448