Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G5S32_RS02370 Genome accession   NZ_CP049331
Coordinates   506778..508466 (-) Length   562 a.a.
NCBI ID   WP_165310306.1    Uniprot ID   A0A6G7CFM6
Organism   Vibrio ziniensis strain ZWAL4003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 501778..513466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5S32_RS02345 (G5S32_02345) yacG 502949..503152 (-) 204 WP_165310301.1 DNA gyrase inhibitor YacG -
  G5S32_RS02350 (G5S32_02350) zapD 503198..503938 (-) 741 WP_165310302.1 cell division protein ZapD -
  G5S32_RS02355 (G5S32_02355) coaE 503970..504578 (-) 609 WP_165310303.1 dephospho-CoA kinase -
  G5S32_RS02360 (G5S32_02360) pilD 504582..505451 (-) 870 WP_165310304.1 A24 family peptidase Machinery gene
  G5S32_RS02365 (G5S32_02365) pilC 505531..506757 (-) 1227 WP_165310305.1 type II secretion system F family protein Machinery gene
  G5S32_RS02370 (G5S32_02370) pilB 506778..508466 (-) 1689 WP_165310306.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G5S32_RS02375 (G5S32_02375) - 508463..508906 (-) 444 WP_165310307.1 pilin -
  G5S32_RS02380 (G5S32_02380) nadC 509135..510022 (-) 888 WP_165310308.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G5S32_RS02385 (G5S32_02385) ampD 510157..510699 (+) 543 WP_165310309.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G5S32_RS02390 (G5S32_02390) fldB 510746..511264 (-) 519 WP_165310310.1 flavodoxin FldB -
  G5S32_RS02395 (G5S32_02395) xerD 511445..512353 (+) 909 WP_165310311.1 site-specific tyrosine recombinase XerD -
  G5S32_RS02400 (G5S32_02400) dsbC 512365..513120 (+) 756 WP_165310312.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62097.29 Da        Isoelectric Point: 6.2956

>NTDB_id=426147 G5S32_RS02370 WP_165310306.1 506778..508466(-) (pilB) [Vibrio ziniensis strain ZWAL4003]
MITNLIALLRQAGLLHSDQEVAVVNHIKVSGDSVPETLIQLGIIQSADLTDYLSKQLGLPIAQLNDNDHSDLCQRLGLRE
LIVQFNAIPIKLDKNQLTIAVSDPTYPNVEQEFRFNTGLNIDLVLADHKSLQAAIRRLYGKSLSNKYEQAKEISQDELAN
LVQLSDDELQSIEDLSQDESPVSRFINQVLLDAVRKGASDIHFEPYENLYRIRLRCDGILVETHRPANHLGRRLAARIKV
LAKLDIAERRLPQDGRIKLHLNQSTAVDIRVSSLPTLWGEKIVLRLLDSSASNLDIDKLGYNSLQKQQYLDALKKPQGMI
LMTGPTGSGKTVSLYAGLQMINTSEINISTAEDPVEINLSGINQVQVQPKIGFGFSDALRSFLRQDPDVVMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNSAAETVIRLANMGVNPVNLATSLSLIIAQRLARRLCPFCKIMINPPVSLQTQFKLPESK
VIYQANRLGCNECTAGYSGRIGIYEVMSFNSELAEAVLSHANASQIEAIAKANGMITLREAGIEKLAQGITSFTELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=426147 G5S32_RS02370 WP_165310306.1 506778..508466(-) (pilB) [Vibrio ziniensis strain ZWAL4003]
ATGATAACCAATCTAATTGCTTTATTGCGCCAAGCTGGCTTACTCCATTCAGATCAAGAAGTTGCAGTGGTTAATCACAT
CAAAGTGTCTGGAGATAGTGTTCCGGAAACATTGATTCAACTAGGTATTATTCAGTCAGCGGATCTTACGGATTATTTGA
GCAAACAACTTGGTCTGCCTATCGCTCAGTTAAACGACAATGATCATTCTGACCTTTGCCAACGCTTGGGATTGAGAGAG
TTAATTGTTCAATTTAATGCAATACCAATAAAATTAGATAAAAATCAATTAACCATTGCTGTATCAGACCCAACATATCC
AAATGTGGAACAAGAGTTTCGTTTTAATACTGGCTTAAATATTGACCTTGTTTTGGCTGATCATAAATCATTACAAGCAG
CCATTCGTCGGCTTTATGGCAAATCTCTTAGCAACAAATATGAACAGGCAAAAGAAATAAGCCAAGATGAACTAGCCAAT
CTGGTTCAGCTCTCCGACGATGAACTACAGTCCATTGAAGATCTCAGTCAGGATGAGTCACCAGTTAGTCGATTTATCAA
TCAGGTATTACTTGATGCAGTTCGAAAAGGCGCATCTGACATTCACTTTGAACCCTACGAAAACCTTTACCGCATACGAC
TTCGCTGTGACGGTATTTTAGTCGAAACACATAGACCAGCTAACCATCTAGGTAGAAGATTGGCGGCTCGAATAAAAGTA
CTGGCTAAACTCGATATTGCCGAACGTCGGTTACCTCAAGACGGAAGAATTAAGCTTCACTTAAATCAGAGTACTGCTGT
TGATATCAGAGTGTCATCACTACCAACATTATGGGGAGAAAAAATCGTGTTACGCCTGCTTGATAGCAGTGCGAGCAACC
TCGACATTGATAAGCTAGGCTATAACTCGTTACAAAAGCAGCAATACTTAGATGCGCTAAAAAAACCACAAGGCATGATT
CTAATGACAGGGCCTACAGGAAGCGGTAAAACCGTATCACTGTATGCTGGTCTACAAATGATCAATACTTCTGAAATAAA
TATATCAACCGCAGAAGATCCGGTTGAAATAAACCTCTCTGGTATTAATCAGGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTTCTGATGCTCTGCGCTCTTTCTTACGACAAGATCCAGATGTCGTGATGGTTGGCGAAATTAGAGATTTAGAAACT
GCCGAAATTGCAATTAAAGCAGCTCAAACAGGGCATCTTGTACTATCAACTCTTCACACAAACTCAGCAGCAGAGACAGT
GATTCGTTTAGCCAATATGGGAGTTAACCCGGTAAACCTAGCCACCTCATTAAGCCTGATAATTGCACAACGTTTAGCTC
GTAGATTGTGCCCATTTTGCAAAATCATGATAAACCCACCAGTTAGTCTTCAAACTCAGTTTAAATTGCCAGAGAGCAAA
GTCATTTACCAAGCGAACCGCTTAGGATGCAACGAATGTACCGCTGGCTATTCGGGTAGAATCGGCATTTACGAAGTCAT
GTCATTTAATTCTGAACTTGCTGAGGCTGTATTGAGCCACGCCAATGCCTCACAAATAGAGGCAATAGCAAAAGCCAATG
GCATGATAACGCTACGTGAAGCAGGGATTGAAAAGTTAGCACAAGGGATCACGAGTTTTACTGAGTTACAGCGTGTTCTG
TATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G7CFM6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

74.199

100

0.742
  pilB Vibrio campbellii strain DS40M4

67.438

100

0.674
  pilB Vibrio parahaemolyticus RIMD 2210633

67.082

100

0.671
  pilB Acinetobacter baylyi ADP1

47.398

95.73

0.454
  pilB Legionella pneumophila strain ERS1305867

48.924

90.925

0.445
  pilB Acinetobacter baumannii D1279779

48.356

91.993

0.445
  pilF Neisseria gonorrhoeae MS11

44.03

95.374

0.42
  pilF Thermus thermophilus HB27

36.888

100

0.375