Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   G5S32_RS02360 Genome accession   NZ_CP049331
Coordinates   504582..505451 (-) Length   289 a.a.
NCBI ID   WP_165310304.1    Uniprot ID   A0A6G7CFM1
Organism   Vibrio ziniensis strain ZWAL4003     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 499582..510451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5S32_RS02340 (G5S32_02340) parC 500431..502710 (+) 2280 WP_165310300.1 DNA topoisomerase IV subunit A -
  G5S32_RS02345 (G5S32_02345) yacG 502949..503152 (-) 204 WP_165310301.1 DNA gyrase inhibitor YacG -
  G5S32_RS02350 (G5S32_02350) zapD 503198..503938 (-) 741 WP_165310302.1 cell division protein ZapD -
  G5S32_RS02355 (G5S32_02355) coaE 503970..504578 (-) 609 WP_165310303.1 dephospho-CoA kinase -
  G5S32_RS02360 (G5S32_02360) pilD 504582..505451 (-) 870 WP_165310304.1 A24 family peptidase Machinery gene
  G5S32_RS02365 (G5S32_02365) pilC 505531..506757 (-) 1227 WP_165310305.1 type II secretion system F family protein Machinery gene
  G5S32_RS02370 (G5S32_02370) pilB 506778..508466 (-) 1689 WP_165310306.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G5S32_RS02375 (G5S32_02375) - 508463..508906 (-) 444 WP_165310307.1 pilin -
  G5S32_RS02380 (G5S32_02380) nadC 509135..510022 (-) 888 WP_165310308.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 289 a.a.        Molecular weight: 32252.25 Da        Isoelectric Point: 7.0547

>NTDB_id=426145 G5S32_RS02360 WP_165310304.1 504582..505451(-) (pilD) [Vibrio ziniensis strain ZWAL4003]
MEIFDYYPWLFVAFASLLGLIIGSFLNVVIYRLPKIMEREWRQECVDYFPESNIQPPEGKLTLSTPRSTCPSCNTPLRII
DNIPVISWLVLRGKCAHCQTSISARYPLIELLTAAISFVVAQHFGFSYFTIALLFFSYVLIAGTFIDLDTMLLPDQLTLP
LLWGGIALSLIGISSVSLQDAIIGAIAGYLALWSVYWGFKLATGKEGMGYGDFKLLAALGAWLGWQLLPLIILLSSLVGL
VFGIIQLRLKKQGIDKAFPFGPYIAIAGWIALLWGNQIIDRYYAYLLGY

Nucleotide


Download         Length: 870 bp        

>NTDB_id=426145 G5S32_RS02360 WP_165310304.1 504582..505451(-) (pilD) [Vibrio ziniensis strain ZWAL4003]
ATGGAAATTTTTGACTACTATCCTTGGCTTTTCGTAGCATTTGCCTCCCTTCTTGGTTTAATTATTGGCAGTTTTCTTAA
CGTAGTGATTTACCGACTGCCGAAAATCATGGAACGTGAATGGCGTCAGGAATGTGTTGACTACTTTCCTGAATCCAATA
TTCAACCACCAGAAGGCAAACTGACACTAAGCACACCGCGTTCAACCTGCCCTAGTTGCAACACGCCACTACGTATTATC
GACAATATTCCTGTTATTAGCTGGTTAGTTTTACGTGGTAAATGCGCTCACTGTCAAACATCCATCAGTGCTCGTTATCC
TTTGATTGAGCTGCTAACGGCTGCTATCAGCTTTGTTGTTGCACAACACTTTGGTTTTAGCTATTTCACTATTGCCTTGC
TGTTTTTCAGCTATGTCCTTATTGCCGGAACCTTTATCGATTTAGATACCATGTTGTTGCCGGACCAACTCACTCTGCCG
CTACTTTGGGGAGGCATCGCTCTTTCACTTATAGGTATTAGCTCTGTCTCTTTACAAGATGCCATTATTGGCGCTATTGC
GGGTTACTTAGCTCTTTGGTCTGTATATTGGGGTTTCAAGCTCGCAACGGGCAAAGAAGGGATGGGATACGGCGATTTTA
AACTACTTGCAGCTCTTGGGGCTTGGTTAGGCTGGCAGTTATTACCATTGATAATTCTGCTTTCATCTCTTGTGGGTCTT
GTGTTCGGTATTATTCAATTGCGATTGAAAAAACAAGGTATTGATAAAGCTTTTCCATTTGGGCCTTACATTGCGATTGC
TGGATGGATAGCTTTATTGTGGGGAAACCAGATTATTGATAGATACTACGCTTACCTATTGGGGTACTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(135-244)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G7CFM1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

74.653

99.654

0.744
  pilD Vibrio cholerae strain A1552

73.958

99.654

0.737
  pilD Acinetobacter baumannii D1279779

57.299

94.81

0.543
  pilD Acinetobacter nosocomialis M2

57.299

94.81

0.543
  pilD Neisseria gonorrhoeae MS11

49.807

89.619

0.446