Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G4Q83_RS21100 Genome accession   NZ_CP049017
Coordinates   4518542..4520278 (+) Length   578 a.a.
NCBI ID   WP_128420134.1    Uniprot ID   A0A2S6ZFQ2
Organism   Xanthomonas theicola strain CFBP 4691     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4513542..4525278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4Q83_RS21080 (G4Q83_21160) coaE 4514796..4515416 (-) 621 WP_128420138.1 dephospho-CoA kinase -
  G4Q83_RS21085 (G4Q83_21165) - 4515430..4516293 (-) 864 WP_128420137.1 A24 family peptidase -
  G4Q83_RS21090 (G4Q83_21170) pilC 4516300..4517556 (-) 1257 WP_128420136.1 type II secretion system F family protein Machinery gene
  G4Q83_RS21095 (G4Q83_21175) pilE 4517937..4518398 (+) 462 WP_128420135.1 pilin Machinery gene
  G4Q83_RS21100 (G4Q83_21180) pilB 4518542..4520278 (+) 1737 WP_128420134.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G4Q83_RS21105 (G4Q83_21185) pilR 4520427..4521812 (-) 1386 WP_128420133.1 sigma-54 dependent transcriptional regulator Regulator
  G4Q83_RS21110 (G4Q83_21190) - 4521892..4523499 (-) 1608 WP_128420132.1 HAMP domain-containing sensor histidine kinase -
  G4Q83_RS21115 (G4Q83_21195) sucC 4523741..4524904 (+) 1164 WP_128420131.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62749.00 Da        Isoelectric Point: 6.4309

>NTDB_id=424338 G4Q83_RS21100 WP_128420134.1 4518542..4520278(+) (pilB) [Xanthomonas theicola strain CFBP 4691]
MNALTSANLVGITGIARRLVQDGAMEEAVARTAMAHAAEAKIPLPQWFAEKKLVTAAQLAAANAVEFGMPLMDVSVFDAN
QHAMKLVSEELLHKHQVLPLFKRGTRLFVGISNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDSLGNGLG
GDDEGMDNLEVGAGDEDMGSGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVARAP
VKLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPTQLP
ENALLAEGFTAAEVAAGVKLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIAAAAQRIGVKDLRQSALR
KACNGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=424338 G4Q83_RS21100 WP_128420134.1 4518542..4520278(+) (pilB) [Xanthomonas theicola strain CFBP 4691]
ATGAATGCTCTGACATCCGCCAACCTGGTCGGCATCACCGGCATCGCCCGCCGCCTGGTTCAGGATGGCGCGATGGAGGA
AGCCGTCGCCCGCACCGCCATGGCCCATGCCGCCGAGGCCAAGATCCCGCTGCCGCAGTGGTTTGCGGAGAAGAAGCTGG
TCACCGCCGCGCAGCTGGCGGCGGCCAATGCGGTGGAGTTCGGCATGCCGTTGATGGATGTCTCGGTGTTCGACGCCAAC
CAGCATGCGATGAAGCTGGTCAGCGAGGAGCTGCTGCACAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCACGCGTCT
GTTCGTGGGAATCAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAATCTCACCGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGGCGCACCCTGGAGCAGTGGCAGGCGAGCAACGATTCGCTCGGCAATGGGCTGGGC
GGCGACGACGAGGGCATGGACAACCTCGAGGTCGGGGCCGGCGACGAGGACATGGGCAGCGGCGGCGATAGCGGCGTCGA
TGCCAAGGGCGACGACACGCCCGTGGTCAAGTTCGTGAACAAGGTGCTGGTCGATGCGATCCGCCGCGGCGCCTCGGACA
TCCATTTCGAGCCGTACGAGGACGACTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAGGGCGCCG
GTCAAGCTCAACCAGCGCATCGCGGCGCGGTTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACGTTGTTCGGCGAGA
AGATCGTGCTGCGCATCCTGGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCCACCGGTTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGCATCCTCAACGACGACACCCGCAACATCTCCACCGCCGAGGACCCGGTGGAAATCCGCCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGTTCGTTCCTGCGACAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGGCACATGGT
GCTGTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGGCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGTTCGGTGACCCTGGTGATCGCGCAGCGCCTGGCGCGGCGGCTGTGCAACAACTGCAAGCGCCCGACTCAATTGCCG
GAAAACGCGCTGCTGGCCGAGGGCTTCACCGCGGCGGAAGTCGCCGCCGGCGTCAAGCTGTACGAGGCGGTCGGCTGCGA
CGAGTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTACCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGC
TGCAGGGCGGCAACGCGATGGACATCGCCGCGGCGGCGCAGAGGATCGGGGTCAAGGACCTGCGCCAGTCGGCATTGCGC
AAGGCGTGCAACGGCGTCACCAGCCTGGCCGAGATCAATCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6ZFQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.483

100

0.547
  pilB Acinetobacter baumannii D1279779

54.991

98.789

0.543
  pilB Legionella pneumophila strain ERS1305867

51.845

98.443

0.51
  pilF Neisseria gonorrhoeae MS11

48.415

98.27

0.476
  pilB Vibrio cholerae strain A1552

50.647

93.599

0.474
  pilB Vibrio campbellii strain DS40M4

45.343

98.443

0.446
  pilB Vibrio parahaemolyticus RIMD 2210633

45.583

97.924

0.446