Detailed information NTG0422270

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: G3T13_RS03720
• Genome accession: NZ_CP048686
• Coordinates: 777794..778645 (-)
• Length: 283 a.a.
• NCBI ID: WP_061774798.1
• Uniprot ID: A0A2A5SB33
• Organism: Lactococcus raffinolactis strain KACC 13441
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 772794..783645

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G3T13_RS03700	purB	773547..774839 (-)	1293	WP_061774794.1	adenylosuccinate lyase	-
G3T13_RS03705	purK	774870..775928 (-)	1059	WP_096824057.1	5-(carboxyamino)imidazole ribonucleotide synthase	-
G3T13_RS03710	purE	775921..776415 (-)	495	WP_061774796.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
G3T13_RS03715	purD	776408..777661 (-)	1254	WP_061774797.1	phosphoribosylamine--glycine ligase	-
G3T13_RS03720	dprA	777794..778645 (-)	852	WP_061774798.1	DNA-processing protein DprA	Machinery gene
G3T13_RS03725	-	778727..779683 (-)	957	WP_061774799.1	ABC transporter permease	-
G3T13_RS03730	-	779683..780759 (-)	1077	WP_061774800.1	ABC transporter permease	-
G3T13_RS03735	-	780762..782276 (-)	1515	WP_061774801.1	ABC transporter ATP-binding protein	-
G3T13_RS03740	deoD	782425..783135 (-)	711	WP_061774802.1	purine-nucleoside phosphorylase	-

Sequence

Protein

• Length: 283 a.a.
• Molecular weight: 32073.10 Da
• Isoelectric Point: 8.4451

>NTDB_id=422270 G3T13_RS03720 WP_061774798.1 777794..778645(-) (dprA) [Lactococcus raffinolactis strain KACC 13441]
MQNFDIYRLKKAGMSNLMVYQFLTLVADSQERNPLVCLPAFIRQVKMKQSAHFWEKFRSFDIKAIREEFQKFPSFSIQDK
VYPEALREIYNPPVLLFYQGDLSLLHQTKLGFVGSRDCSQVGVASVQKIIKELQDRFVIVSGLARGIDTASHIAAMKNGG
KTIAVIGTGLDVNYPRENYQLQAYIAKHQLLLSEYGPDEKPLKYHFPERNRIIAGLSRGVLVTEAKLRSGSLITCERAME
EGRDVFAVPGNIIDGVSDGCHHLIQEGAKLIFHGRDILMEYDM

Nucleotide

• Length: 852 bp

>NTDB_id=422270 G3T13_RS03720 WP_061774798.1 777794..778645(-) (dprA) [Lactococcus raffinolactis strain KACC 13441]
ATGCAAAATTTCGATATCTATCGCTTAAAAAAAGCTGGCATGTCAAACTTAATGGTTTATCAGTTTTTAACCCTAGTTGC
AGACAGTCAAGAAAGAAACCCCTTGGTCTGTTTACCAGCCTTTATTCGACAAGTTAAGATGAAACAGTCAGCTCATTTTT
GGGAAAAATTCCGATCATTTGACATAAAAGCCATACGAGAGGAGTTTCAGAAATTTCCGTCTTTCTCAATTCAGGATAAG
GTCTATCCTGAAGCTTTGCGAGAAATTTATAATCCGCCCGTCTTACTTTTTTATCAGGGAGATTTGAGCTTGCTTCATCA
GACCAAACTGGGTTTTGTTGGCAGTCGAGATTGCAGTCAAGTAGGGGTTGCCAGTGTTCAGAAGATCATCAAAGAATTGC
AAGATCGCTTTGTGATTGTTAGTGGCTTAGCACGCGGCATTGATACAGCTAGTCATATTGCTGCTATGAAAAATGGTGGT
AAGACAATTGCTGTGATTGGAACAGGGTTAGATGTGAATTATCCACGAGAAAATTATCAACTGCAGGCTTATATCGCAAA
ACATCAGCTCCTTTTATCAGAATATGGGCCAGATGAAAAACCTTTGAAGTATCATTTTCCAGAGCGTAATCGAATTATTG
CAGGTCTATCTCGTGGTGTATTAGTGACAGAAGCCAAGCTCCGCTCAGGTAGTTTGATTACCTGTGAGCGGGCTATGGAA
GAGGGGAGAGATGTGTTTGCCGTTCCAGGTAATATCATAGATGGGGTGTCAGATGGCTGCCACCATTTGATTCAGGAAGG
TGCAAAACTGATTTTCCATGGTCGAGATATATTAATGGAGTACGACATGTGA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (75-271)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-64)

3D structure

• AlphaFold DB: A0A2A5SB33

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	62.898	100	0.629
dprA	Lactococcus lactis subsp. cremoris KW2	60.498	99.293	0.601
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	58.156	99.647	0.58
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	58.156	99.647	0.58
dprA/cilB/dalA	Streptococcus pneumoniae D39	58.156	99.647	0.58
dprA/cilB/dalA	Streptococcus pneumoniae R6	58.156	99.647	0.58
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	58.156	99.647	0.58
dprA/cilB/dalA	Streptococcus mitis SK321	57.801	99.647	0.576
dprA	Bacillus subtilis subsp. subtilis str. 168	49.321	78.092	0.385
dprA	Legionella pneumophila strain ERS1305867	40.23	92.226	0.371
dprA	Staphylococcus aureus MW2	35.664	100	0.36