Detailed information NTG0422237

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: G0030_RS06670
• Genome accession: NZ_CP048681
• Coordinates: 1411642..1412835 (+)
• Length: 397 a.a.
• NCBI ID: WP_045794791.1
• Uniprot ID: A0A6C0Y670
• Organism: Acinetobacter indicus strain Q186-3_T
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1406642..1417835

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G0030_RS06655 (G0030_06640)	-	1406866..1408248 (-)	1383	WP_016658082.1	amino acid permease	-
G0030_RS06660 (G0030_06645)	hppD	1408400..1409458 (-)	1059	WP_168383124.1	4-hydroxyphenylpyruvate dioxygenase	-
G0030_RS06665 (G0030_06650)	-	1410333..1411388 (+)	1056	WP_075166576.1	3-deoxy-7-phosphoheptulonate synthase	-
G0030_RS06670 (G0030_06655)	pilU	1411642..1412835 (+)	1194	WP_045794791.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
G0030_RS06675 (G0030_06660)	-	1412894..1414075 (-)	1182	WP_016658086.1	MFS transporter	-
G0030_RS06680 (G0030_06665)	gcvH	1414331..1414705 (-)	375	WP_005178176.1	glycine cleavage system protein GcvH	-
G0030_RS06685 (G0030_06670)	-	1414774..1415802 (-)	1029	WP_005178175.1	AraC family transcriptional regulator	-
G0030_RS06690 (G0030_06675)	-	1415906..1416355 (-)	450	WP_016658087.1	MaoC family dehydratase	-

Sequence

Protein

• Length: 397 a.a.
• Molecular weight: 44672.05 Da
• Isoelectric Point: 6.2356

>NTDB_id=422237 G0030_RS06670 WP_045794791.1 1411642..1412835(+) (pilU) [Acinetobacter indicus strain Q186-3_T]
MYSAELLEDARKFMFHMLSKVVEFGGSDLFITADFPPSIKHQGMMKALGQQALTAEKTKLFAYSLMNEKQRSEFETDLEC
NFAISVPEVSRFRVNVFQQQLHVGMVIRTISAEIPNFSQLKLPESLKHIIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDSHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTEDGKGRRAAVEIMLNTPLISELILKGQFHELKSIMGKS
RELGMQTFDQALFDLYNEGAISYEEAIRNADSMNELRLQIKLKSNRPSEASAAMSFSMLEEPKPEDEDTESAPQQSA

Nucleotide

• Length: 1194 bp

>NTDB_id=422237 G0030_RS06670 WP_045794791.1 1411642..1412835(+) (pilU) [Acinetobacter indicus strain Q186-3_T]
ATGTATAGTGCAGAATTACTCGAAGATGCGCGTAAATTTATGTTTCATATGCTCAGCAAAGTGGTTGAGTTTGGGGGATC
AGATTTATTTATTACCGCGGATTTTCCACCGAGCATTAAACATCAGGGCATGATGAAAGCGCTGGGACAACAGGCGCTGA
CTGCAGAAAAAACCAAGCTGTTTGCCTACAGCCTCATGAATGAAAAACAGCGTAGCGAGTTTGAAACTGATCTGGAATGC
AACTTTGCCATTAGTGTGCCGGAGGTATCGCGTTTTCGGGTCAATGTGTTTCAACAGCAGCTGCATGTCGGGATGGTGAT
TCGAACCATTAGCGCGGAAATTCCAAACTTTAGCCAGCTGAAACTGCCGGAATCACTGAAGCATATTATTATGGAAAAGC
GCGGTCTGGTGCTGGTGGTGGGGGGAACCGGTTCAGGTAAATCGACCTCTTTGGCGGCGATGATTGATCATCGTAATGAA
AATTCAGCCGGGCATATTATTACCGTGGAAGACCCGGTGGAATATGTGCACAAGCATAAAAAGTCGATGATTACCCATCG
TGAAGTCGGGGTCGACAGCCATTCCTGGCACAATGCGCTGAAAAATACCTTACGTCAGGCACCGGATGTGATTTTAATTG
GCGAGATCCGCGACACCGAAACCATGGAACATGCGATTGCCTTTGCTGAAACCGGGCATTTATGTTTGGGCACCTTGCAT
GCCAATAACGCCAACCAGACGCTGGACCGGATTATCAACTTTTTCCCGGAAGAACGCCGTAACCAGTTATTGATGGACCT
GTCGTCCAATATGAAGGCCATTATTTCCCAGCGTCTGGTGCGTACCGAAGATGGTAAAGGCCGCCGTGCGGCAGTCGAGA
TTATGCTGAATACGCCGCTGATTTCTGAACTGATTTTAAAAGGCCAGTTCCATGAGTTAAAGTCGATTATGGGCAAATCG
CGTGAACTGGGCATGCAGACGTTTGATCAGGCTCTATTTGATTTGTATAACGAAGGCGCGATTTCCTATGAAGAAGCAAT
TCGTAATGCCGATTCGATGAATGAATTGCGCTTACAGATTAAATTGAAAAGTAACCGGCCAAGCGAAGCTTCGGCAGCTA
TGAGCTTTAGCATGCTGGAAGAGCCAAAACCGGAAGATGAAGATACGGAATCTGCTCCTCAACAGAGTGCTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (117-282)

3D structure

• AlphaFold DB: A0A6C0Y670

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.568	93.199	0.537
pilU	Acinetobacter baylyi ADP1	54.42	91.184	0.496
pilU	Vibrio cholerae strain A1552	52.663	85.139	0.448
pilT	Acinetobacter baumannii strain A118	43.939	83.123	0.365
pilT	Acinetobacter nosocomialis M2	43.939	83.123	0.365
pilT	Acinetobacter baumannii D1279779	43.939	83.123	0.365
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.814	84.131	0.36