Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G0028_RS17405 Genome accession   NZ_CP048659
Coordinates   3508813..3510528 (+) Length   571 a.a.
NCBI ID   WP_174493874.1    Uniprot ID   -
Organism   Acinetobacter piscicola strain YH12207_T     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3503813..3515528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G0028_RS17365 (G0028_17340) nusA 3504372..3505856 (-) 1485 WP_130072255.1 transcription termination factor NusA -
  G0028_RS17370 (G0028_17345) rimP 3505897..3506421 (-) 525 WP_130072256.1 ribosome maturation factor RimP -
  G0028_RS17395 (G0028_17370) secG 3507232..3507564 (-) 333 WP_130072257.1 preprotein translocase subunit SecG -
  G0028_RS17400 (G0028_17375) tpiA 3507574..3508368 (-) 795 WP_180046476.1 triose-phosphate isomerase -
  G0028_RS17405 (G0028_17380) pilB 3508813..3510528 (+) 1716 WP_174493874.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G0028_RS17410 (G0028_17385) pilC 3510555..3511781 (+) 1227 WP_130072260.1 type II secretion system F family protein Machinery gene
  G0028_RS17415 (G0028_17390) pilD 3511781..3512644 (+) 864 WP_130072261.1 prepilin peptidase Machinery gene
  G0028_RS17420 (G0028_17395) coaE 3512656..3513252 (+) 597 WP_180046477.1 dephospho-CoA kinase -
  G0028_RS17425 (G0028_17400) - 3513320..3514237 (-) 918 WP_130072263.1 DMT family transporter -
  G0028_RS17430 (G0028_17405) rlmB 3514280..3515032 (-) 753 WP_174493878.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  G0028_RS17435 (G0028_17410) - 3515172..3515495 (-) 324 WP_065993789.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63426.83 Da        Isoelectric Point: 4.9033

>NTDB_id=422178 G0028_RS17405 WP_174493874.1 3508813..3510528(+) (pilB) [Acinetobacter piscicola strain YH12207_T]
MVSQQGTPKFSGFIRRLVDDGVIAAQQMQSALDNAKKSKLNIVAYLIEYANLKPLQIAETIALEFAEPLFDLDSFDPEQF
PKDVIESGLLHKHLMLPLVQRGNVLYIAMSDPTNIEALDAARFNSRLNIEVVIVEHHKLVKCLEKYFTEDTSFDFGGDDI
DVDIISTDDVNPADDHDDNESEDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRSITNPPLQLATRLA
SRLKVMSQMDISEKRIPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALDK
PQGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSVALKSFLRQDPDIIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCTHCKAPVEIPKHSLLEMG
FTEEDLAHPDFQVFQPVGCPECREGYKGRVGVYEVMKVTPEISKIIMEDGNALEIAEASAKLGFNNLRRSGLIKVMNGLT
SLQEINRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=422178 G0028_RS17405 WP_174493874.1 3508813..3510528(+) (pilB) [Acinetobacter piscicola strain YH12207_T]
ATGGTGTCACAACAAGGCACTCCGAAGTTTTCGGGGTTTATTCGTCGCTTAGTCGATGACGGTGTTATTGCTGCGCAACA
AATGCAATCTGCGCTAGACAATGCTAAAAAAAGTAAGCTCAACATCGTAGCTTATCTGATTGAATATGCAAATTTAAAAC
CTTTGCAAATTGCTGAAACCATTGCGCTTGAGTTCGCCGAGCCTTTGTTTGACCTTGATAGCTTTGATCCCGAGCAATTC
CCTAAAGATGTTATTGAAAGTGGTTTATTACATAAACATCTGATGCTGCCTTTGGTTCAACGTGGAAATGTGCTTTATAT
CGCCATGAGCGATCCAACCAATATTGAGGCTTTGGATGCTGCACGTTTTAATAGTCGCTTGAATATTGAAGTCGTCATCG
TTGAACATCACAAACTCGTCAAATGCTTAGAAAAATACTTTACTGAAGACACCAGTTTTGATTTTGGTGGTGATGATATT
GATGTTGATATCATCTCAACTGATGATGTAAATCCTGCAGATGATCACGACGATAATGAAAGCGAAGATGAAGCTCCGAT
TGTAAAGTATATTAATAAGTTACTTATTGATGCTATTCGTATGGGTGCATCAGATTTACATTTTGAACCTTATGAAAAAT
CCTATCGTGTGCGTTATCGTGTAGATGGTGTGCTTAGAAGTATTACCAATCCCCCACTGCAACTTGCCACACGACTCGCA
TCACGCTTAAAAGTGATGTCACAAATGGATATTTCTGAAAAGCGTATTCCACAAGATGGTCGTATTAAACTTAAATTATC
CAAAACCAAAGCCATCGACTTTCGTGTCAACTCTCTCCCGACGCTATTTGGTGAAAAACTCGTACTGCGTATTTTAGATC
CATCAAGTGCGATGCTAGGTATTGATGCTCTAGGTTATGAGGAAGATCAAAAAGCTTTATTCATGGAAGCTTTAGATAAA
CCTCAAGGTATGCTCTTAATCACAGGACCGACAGGCTCTGGTAAAACGGTATCACTCTATACAGGTTTAAATATTTTAAA
TACTGAAAGTTCTAATATTTCAACAGCCGAAGACCCTGTAGAGATTAACTTAGAAGGGATTAATCAGGTCAATGTTAATC
CTAAAGTCGGACTGACGTTTTCTGTCGCCTTGAAATCTTTCTTACGTCAAGACCCTGATATCATTATGGTCGGGGAGATT
CGAGACCTTGAAACTGCTGAAATTGCGATTAAAGCAGCTCAAACAGGTCATATGGTCATGTCTACACTACATACCAATAG
TGCGCCTGAAACCTTAACACGTTTAAGAAATATGGGCGTTCCTTCTTTTAATATTGCAACCTCTGTCAATCTCGTCATTG
CACAACGTTTGGCACGCCGCTTATGTACACATTGTAAAGCACCTGTTGAAATTCCTAAACATAGCCTCTTAGAAATGGGC
TTTACTGAAGAGGATTTAGCACACCCTGATTTCCAAGTGTTTCAACCTGTGGGTTGCCCAGAATGTCGTGAAGGCTATAA
AGGGCGTGTTGGGGTATATGAAGTGATGAAAGTGACACCTGAGATTTCAAAAATTATTATGGAAGATGGTAATGCCTTAG
AAATTGCTGAAGCATCTGCCAAACTGGGGTTTAATAATTTACGACGCTCAGGACTGATTAAAGTCATGAATGGTTTAACA
TCACTACAAGAAATTAATCGCGTCACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.035

100

0.8
  pilB Acinetobacter baylyi ADP1

76.773

98.774

0.758
  pilF Neisseria gonorrhoeae MS11

50

99.475

0.497
  pilB Legionella pneumophila strain ERS1305867

51.654

95.271

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

48.571

98.074

0.476
  pilB Vibrio cholerae strain A1552

47.321

98.074

0.464
  pilB Vibrio campbellii strain DS40M4

47.038

97.548

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

35.294

100

0.378

Multiple sequence alignment