Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G0027_RS06465 Genome accession   NZ_CP048654
Coordinates   1351298..1353004 (+) Length   568 a.a.
NCBI ID   WP_016659655.1    Uniprot ID   A0A6C0YJ55
Organism   Acinetobacter indicus strain C15_T     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1346298..1358004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G0027_RS06425 (G0027_06435) nusA 1346963..1348447 (-) 1485 WP_005181786.1 transcription termination factor NusA -
  G0027_RS06430 (G0027_06440) rimP 1348480..1349004 (-) 525 WP_005181784.1 ribosome maturation factor RimP -
  G0027_RS06455 (G0027_06465) secG 1349874..1350203 (-) 330 WP_005181783.1 preprotein translocase subunit SecG -
  G0027_RS06460 (G0027_06470) tpiA 1350217..1351008 (-) 792 WP_005181780.1 triose-phosphate isomerase -
  G0027_RS06465 (G0027_06475) pilB 1351298..1353004 (+) 1707 WP_016659655.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G0027_RS06470 (G0027_06480) pilC 1353035..1354261 (+) 1227 WP_127801106.1 type II secretion system F family protein Machinery gene
  G0027_RS06475 (G0027_06485) pilD 1354261..1355121 (+) 861 WP_127801107.1 A24 family peptidase Machinery gene
  G0027_RS06480 (G0027_06490) coaE 1355132..1355740 (+) 609 WP_005181773.1 dephospho-CoA kinase -
  G0027_RS06485 (G0027_06495) - 1355737..1356657 (-) 921 WP_016659657.1 DMT family transporter -
  G0027_RS06490 (G0027_06500) rlmB 1356675..1357427 (-) 753 WP_005181769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  G0027_RS06495 (G0027_06505) - 1357533..1357856 (-) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63758.17 Da        Isoelectric Point: 4.8722

>NTDB_id=422116 G0027_RS06465 WP_016659655.1 1351298..1353004(+) (pilB) [Acinetobacter indicus strain C15_T]
MSVQQAMPRFTGFIRRLVEEGLVTAENMQQAIVAAKKAETDIVPYLIQQLQISPLTIAEKISYEFGEPIFDLAVYDSRLI
VREGLDEKLFTKYRILPIFRRHNQLYIATSNPTNIEAIDAIRFNSKLNIEVVIVEHDKLERLIEQNFTEDSTFDFDEDFD
LEVGDNAPETQEDNDEPQGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRIDGVLRQIANPPLQLATRLASRL
KVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQG
MLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCQKCKAPVEIPRQSLLELGFTE
EDLNQPEFQLYQPVGCPECREGYKGRVGVYEVMKVTPEISRIIMEDGNALQIAAASAKSGFNNLRRSGLIKVMQGVTSLQ
EVNRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=422116 G0027_RS06465 WP_016659655.1 1351298..1353004(+) (pilB) [Acinetobacter indicus strain C15_T]
ATGTCAGTACAACAAGCAATGCCTAGATTTACAGGTTTTATTCGGCGACTGGTCGAAGAAGGGCTTGTGACGGCTGAAAA
TATGCAGCAAGCCATTGTCGCGGCGAAAAAAGCCGAGACCGATATTGTGCCGTATTTAATTCAGCAGTTGCAGATTTCAC
CGCTGACCATTGCTGAAAAAATTTCCTATGAATTTGGTGAACCGATTTTTGACTTGGCGGTGTACGATTCGCGTCTGATT
GTACGTGAAGGTCTGGATGAAAAACTGTTCACCAAATACCGAATTCTGCCCATTTTCCGACGTCACAATCAGCTTTATAT
TGCTACCAGTAACCCAACCAATATCGAAGCCATTGATGCGATCCGCTTTAACAGCAAGCTGAATATTGAAGTGGTGATTG
TTGAGCATGACAAGCTGGAACGGCTGATTGAACAGAACTTTACCGAAGACAGCACCTTTGACTTTGATGAAGACTTTGAT
CTAGAAGTTGGCGACAACGCTCCCGAAACACAAGAAGATAACGATGAGCCACAAGGCGATGAAGCCCCAATCGTCAAATA
TATTAACAAGTTACTGGTCGATGCAATCCGTATGGGCGCCTCGGATTTACACTTCGAGCCTTATGAAAAAAGCTACCGGG
TCCGTTACCGGATTGACGGCGTACTGCGCCAGATTGCCAATCCTCCGTTACAGCTGGCCACACGGCTCGCCTCACGTTTA
AAAGTTATGTCACAAATGGACATCTCGGAAAAACGTATTCCGCAGGATGGCCGGATCAAGCTCAAGCTGTCAAAAAACAA
AGCCATCGACTTCCGTGTCAACTCACTGCCCACCCTGTTCGGGGAAAAAATTGTACTGCGTATTCTCGATCCATCTAGTG
CCATGCTGGGGATTGATGCACTCGGTTATGAACCGGAGCAAAAAGAGCTGTTTATGGAGGCGCTGGACAAACCACAAGGC
ATGTTGCTGATTACCGGTCCGACCGGTTCTGGTAAAACCGTGTCTTTATATACCGGCTTAAATATCCTCAACCGCGAAGA
CACCAATATTTCTACCGCCGAAGATCCGGTGGAAATTAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGAAAGTGG
GTCTGACCTTTGCCGCAGCACTAAAATCATTCCTGCGCCAGGATCCGGATATTGTCATGGTCGGTGAGATCCGTGATCTG
GAAACTGCAGAAATTGCCATTAAAGCCGCACAAACCGGTCACATGGTCATGTCGACGCTGCACACCAACAGTGCACCCGA
AACCCTGACCCGTTTACGCAACATGGGCGTCCCCTCATTTAACATCGCCACCTCGGTGAACCTGGTGATTGCCCAGCGTC
TGGCACGTCGTCTCTGCCAGAAATGCAAGGCCCCGGTGGAGATTCCACGCCAGAGCCTCTTGGAACTCGGTTTTACCGAA
GAAGATTTAAACCAGCCAGAGTTCCAGCTGTATCAGCCGGTTGGCTGCCCGGAATGTCGTGAAGGTTATAAAGGCCGTGT
CGGGGTCTATGAAGTAATGAAAGTCACCCCGGAAATCTCACGCATTATTATGGAAGATGGCAATGCCTTGCAGATTGCAG
CAGCCTCGGCAAAATCCGGGTTTAACAATTTACGCCGTTCAGGATTAATCAAGGTCATGCAGGGGGTCACTTCTTTACAG
GAAGTGAATCGTGTGACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6C0YJ55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.526

100

0.808
  pilB Acinetobacter baylyi ADP1

78.07

100

0.783
  pilF Neisseria gonorrhoeae MS11

51.964

98.592

0.512
  pilB Legionella pneumophila strain ERS1305867

50.53

99.648

0.504
  pilB Vibrio cholerae strain A1552

49.912

100

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

49.553

98.415

0.488
  pilB Vibrio campbellii strain DS40M4

50.864

91.725

0.467
  pilF Thermus thermophilus HB27

37.258

100

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

33.821

100

0.366

Multiple sequence alignment