Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   G3M63_RS07630 Genome accession   NZ_CP048629
Coordinates   1633710..1634435 (-) Length   241 a.a.
NCBI ID   WP_163984209.1    Uniprot ID   A0A6C0TVK0
Organism   Pseudomonas sp. OIL-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Integron 1630010..1633516 1633710..1634435 flank 194


Gene organization within MGE regions


Location: 1630010..1634435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M63_RS07615 (G3M63_07615) - 1630010..1631164 (+) 1155 WP_163982411.1 hypothetical protein -
  G3M63_RS07620 (G3M63_07620) - 1631282..1631899 (+) 618 WP_163982413.1 HNH endonuclease -
  G3M63_RS07625 (G3M63_07625) - 1632017..1633516 (+) 1500 WP_163982415.1 ATP/GTP-binding protein -
  G3M63_RS07630 (G3M63_07630) pilF 1633710..1634435 (-) 726 WP_163984209.1 GspE/PulE family protein Machinery gene

Sequence

Protein


Download         Length: 241 a.a.        Molecular weight: 26372.17 Da        Isoelectric Point: 6.4911

>NTDB_id=421971 G3M63_RS07630 WP_163984209.1 1633710..1634435(-) (pilF) [Pseudomonas sp. OIL-1]
MVTGPTGCGKSTTLYAAINDIRKSGVNIITVEDPVEFQIEGIRQIQVNASIGYTFARALRHILRHDPDVIMVGEIRDQET
AVMASESALTGHLVLSTLHTNSAASSVTRLLEIGIPPYLVNATLTGVLAQRLARRNCPHCLVDEEVPDHVRESLGLSSDE
RFSVGKGCDRCDNRGFAGRIAVYELLEVTLGVRRLIKANVAAQDIEEQARRDGMRPLTEGALELARKGTISLAEVYRIRL
E

Nucleotide


Download         Length: 726 bp        

>NTDB_id=421971 G3M63_RS07630 WP_163984209.1 1633710..1634435(-) (pilF) [Pseudomonas sp. OIL-1]
CTGGTCACCGGTCCCACCGGGTGCGGCAAGAGCACCACCCTGTACGCTGCGATCAACGATATCCGCAAGAGCGGCGTGAA
CATCATCACCGTGGAAGACCCCGTAGAATTCCAGATTGAAGGCATTCGCCAGATCCAGGTGAACGCTTCCATCGGCTACA
CCTTTGCCCGCGCCCTGCGTCACATATTGCGCCACGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCAGGAAACC
GCCGTCATGGCCAGCGAAAGCGCGCTGACCGGACACCTGGTACTGAGCACCCTGCATACCAATAGCGCGGCCAGCTCGGT
GACGCGACTGCTGGAAATCGGCATCCCTCCGTATCTGGTCAACGCCACCCTCACCGGCGTGCTCGCGCAACGCCTGGCGC
GACGCAACTGCCCGCACTGTCTGGTCGATGAAGAGGTGCCTGACCACGTTCGTGAGAGCCTGGGCCTCAGCAGCGACGAG
CGCTTCAGCGTCGGCAAGGGCTGCGACCGGTGCGACAATCGCGGCTTTGCCGGGCGTATCGCCGTATACGAACTACTGGA
AGTCACCCTGGGAGTGCGCCGCCTGATCAAGGCTAATGTCGCCGCCCAGGACATTGAAGAGCAGGCCCGCCGGGACGGCA
TGCGCCCGCTGACCGAAGGTGCCCTGGAACTAGCGCGCAAGGGCACCATCTCCTTGGCCGAGGTCTACCGGATACGGCTC
GAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(1-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6C0TVK0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

51.046

99.17

0.506
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.502

100

0.469
  pilB Vibrio cholerae strain A1552

44.309

100

0.452
  pilB/pilB1 Synechocystis sp. PCC 6803

41.245

100

0.44
  pilB Legionella pneumophila strain ERS1305867

41.87

100

0.427
  pilB Acinetobacter baumannii D1279779

41.296

100

0.423
  pilB Acinetobacter baylyi ADP1

40.891

100

0.419
  pilF Neisseria gonorrhoeae MS11

41.494

100

0.415
  ctsE Campylobacter jejuni subsp. jejuni 81-176

42.017

98.755

0.415
  pilB Vibrio parahaemolyticus RIMD 2210633

40.833

99.585

0.407
  pilB Vibrio campbellii strain DS40M4

40.417

99.585

0.402
  pilB Glaesserella parasuis strain SC1401

48.148

78.423

0.378

Multiple sequence alignment