Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BURJV3_RS16225 Genome accession   NC_015947
Coordinates   3542792..3544522 (-) Length   576 a.a.
NCBI ID   WP_014038263.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia JV3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3537792..3549522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BURJV3_RS16210 (BurJV3_3198) sucC 3537897..3539066 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  BURJV3_RS16215 (BurJV3_3199) - 3539472..3541085 (+) 1614 WP_014038261.1 ATP-binding protein -
  BURJV3_RS16220 (BurJV3_3200) pilR 3541146..3542519 (+) 1374 WP_014038262.1 sigma-54 dependent transcriptional regulator Regulator
  BURJV3_RS16225 (BurJV3_3201) pilB 3542792..3544522 (-) 1731 WP_014038263.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BURJV3_RS20975 (BurJV3_3202) - 3544747..3545154 (-) 408 WP_014038264.1 pilin -
  BURJV3_RS16230 (BurJV3_3203) pilA/pilAI 3545258..3545671 (-) 414 WP_014038265.1 pilin Machinery gene
  BURJV3_RS16235 (BurJV3_3204) pilC 3546028..3547287 (+) 1260 WP_014038266.1 type II secretion system F family protein Machinery gene
  BURJV3_RS16240 (BurJV3_3205) - 3547295..3548158 (+) 864 WP_005418626.1 A24 family peptidase -
  BURJV3_RS16245 (BurJV3_3206) coaE 3548170..3548781 (+) 612 WP_014038267.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62277.19 Da        Isoelectric Point: 5.8733

>NTDB_id=42161 BURJV3_RS16225 WP_014038263.1 3542792..3544522(-) (pilB) [Stenotrophomonas maltophilia JV3]
MNAVTTANLVGITGLARRLVQDGALDEASARDAMAKAAAARQPLPTWFAQNKVVGAAQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTFEQWHASHDTLGDALSGD
DDGMGNLDVAAGDEDLGAGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPSNLPEH
ALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGLYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKA
AAGVTSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=42161 BURJV3_RS16225 WP_014038263.1 3542792..3544522(-) (pilB) [Stenotrophomonas maltophilia JV3]
ATGAACGCCGTAACCACCGCCAATCTCGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTCCAGGATGGTGCGCTGGACGA
GGCTTCCGCCCGCGATGCCATGGCCAAGGCCGCTGCGGCCCGTCAGCCGCTGCCGACCTGGTTCGCGCAGAACAAGGTGG
TTGGGGCGGCCCAGTTGGCAGCCGCCAATGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACGTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGCGGCGGCAAACT
GTTCGTGGGCACCAGCAACCCGACCCATTCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTGGAGCCGATCCTGG
TGGACGAGGACCAGATCCGCCGCACATTCGAGCAGTGGCATGCCAGCCATGACACGCTGGGCGACGCCCTGAGCGGCGAC
GACGATGGCATGGGCAACCTGGACGTTGCGGCCGGAGACGAGGATCTCGGCGCCGGTGGCGACTCCGGTATCGATGCCAA
GGGCGATGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGTTGGTGGACGCGATCCGCAAGGGCGCCTCGGACATCCACT
TCGAGCCCTATGAAGACGACTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTGGCGCGCGCGCCGGTGAAG
CTGAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCCGAGAAGCGCGTGCCGCAGGACGGACG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACGCTGCCGACCCTATTCGGTGAAAAGGTGG
TGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTGTTC
CTGGAAGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTATAC
GGCGCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCGTCA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCAGCGTTGCGCTCGTTCCTGCGACAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTC
CACGCTGCATACCAACGATGCACCGCAGACCATCGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCT
CGGTGACCCTGGTGATCGCCCAGCGCCTGGCGCGCCGGCTGTGCAACAACTGCAAGCGCCCGTCCAACCTGCCCGAACAT
GCGCTGCTGGCCGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCGAACTGTACGAGGCGGTCGGCTGTGATGAGTG
CACCGAGGGCTACAAGGGCCGTACCGGTCTCTACCAGGTGATGCCGATGACCGACGAGATTGCCACCATCGTGCTGGCCG
GTGGCAATGCGTTGCAGATTGCCGAGGCCGCACAGCAGATCGGGGTGAACGATCTGCGCCAGTCGGCGTTGAAGAAGGCG
GCTGCGGGTGTCACCAGCCTGGCCGAGATCAATCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.452

100

0.552
  pilB Acinetobacter baumannii D1279779

54.922

100

0.552
  pilB Legionella pneumophila strain ERS1305867

52.021

98.785

0.514
  pilB Vibrio cholerae strain A1552

48.866

99.479

0.486
  pilF Neisseria gonorrhoeae MS11

48.768

98.611

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

45.629

99.306

0.453
  pilB Vibrio campbellii strain DS40M4

44.991

98.785

0.444

Multiple sequence alignment