Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GYM54_RS11165 Genome accession   NZ_CP048411
Coordinates   2429546..2431690 (-) Length   714 a.a.
NCBI ID   WP_181104595.1    Uniprot ID   -
Organism   Pseudomonas sp. MTM4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2424546..2436690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYM54_RS11150 (GYM54_11135) - 2426342..2427856 (-) 1515 WP_197445224.1 SPOR domain-containing protein -
  GYM54_RS11155 (GYM54_11140) aroB 2427866..2428969 (-) 1104 WP_181104591.1 3-dehydroquinate synthase -
  GYM54_RS11160 (GYM54_11145) aroK 2428993..2429538 (-) 546 WP_181104593.1 shikimate kinase AroK -
  GYM54_RS11165 (GYM54_11150) pilQ 2429546..2431690 (-) 2145 WP_181104595.1 type IV pilus secretin PilQ Machinery gene
  GYM54_RS11170 (GYM54_11155) pilP 2431742..2432269 (-) 528 WP_181104597.1 type 4a pilus biogenesis lipoprotein PilP -
  GYM54_RS11175 (GYM54_11160) pilO 2432266..2432889 (-) 624 WP_131651787.1 type 4a pilus biogenesis protein PilO -
  GYM54_RS11180 (GYM54_11165) pilN 2432886..2433458 (-) 573 WP_131651786.1 type 4a pilus biogenesis protein PilN -
  GYM54_RS11185 (GYM54_11170) pilM 2433458..2434522 (-) 1065 WP_181104793.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 76696.86 Da        Isoelectric Point: 4.9448

>NTDB_id=421157 GYM54_RS11165 WP_181104595.1 2429546..2431690(-) (pilQ) [Pseudomonas sp. MTM4]
MNTTLSRLGLSLLAAMISPALLAANLNALDVASLPGDRVELKLAFDEPVPTPRGYTLDQPARIALDLPGVSSKLKSKNHE
LGVGNARSVTVVEAQGRTRLIVNLTSLVPYSTRVDGNNLFVVLGESGAATAAVAAAPVAPLSKAKPTASVAAGKAISNID
FRRGEDGAGNVIITLSDPSISPSIEEQGGKIRVSFAKTQLPESLRVRLDVQDFATPVKFVDSSSKAEGANIAIEPSGLYD
YLAYQTDDKLTISVKPLSESDAEKRKAEHFTYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPW
DQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKASDIARLFQSVTDTDGASG
DRGSISVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGNLWAGDKWNAWGKNGQL
GVADDPNASGKPGDEGYIPSQQVGRFPGIREVPLNTPFVDLGAVGATSGIGLGFVTNNAILDLQLSAMEKTGNGEVVSQP
KVVTADKETAKILKGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDFSQAASTGGIPAIRKN
EVNAKVLVTDGETIVIGGVFSNTQSKSVDKVPFLGDLPFVGRIFRRDLIQDRKSELLVFITPRIMNNQAIQASR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=421157 GYM54_RS11165 WP_181104595.1 2429546..2431690(-) (pilQ) [Pseudomonas sp. MTM4]
ATGAATACCACTCTCTCTCGTCTCGGCTTGTCCCTGCTGGCGGCGATGATTTCGCCCGCGCTGCTGGCGGCGAACCTCAA
TGCACTCGATGTCGCTTCGCTGCCCGGTGACCGCGTTGAACTTAAACTGGCCTTCGATGAGCCTGTGCCCACCCCGCGGG
GTTATACGCTCGACCAGCCGGCGCGGATCGCGCTCGACCTGCCAGGTGTTTCCAGCAAGCTGAAATCCAAAAACCACGAG
CTTGGTGTCGGTAATGCCCGCAGTGTGACCGTAGTCGAGGCCCAAGGTCGCACGCGGCTCATCGTAAACCTCACTTCCTT
GGTGCCTTATTCGACTCGGGTAGACGGAAATAATTTGTTCGTCGTGCTCGGTGAAAGCGGTGCAGCAACCGCGGCTGTAG
CGGCCGCTCCGGTGGCCCCGCTAAGCAAGGCAAAACCGACCGCCTCCGTGGCTGCGGGCAAGGCGATCAGTAACATCGAT
TTCCGTCGTGGGGAAGATGGGGCCGGTAACGTCATCATCACACTGTCGGACCCGTCGATCAGTCCGAGCATCGAAGAGCA
GGGCGGCAAAATCCGGGTTTCATTCGCCAAGACGCAGCTGCCGGAGTCGCTTCGTGTCAGGCTAGACGTGCAGGATTTCG
CTACGCCGGTCAAGTTCGTCGATTCCAGCAGCAAGGCCGAGGGCGCAAACATCGCCATCGAGCCCAGCGGCCTTTACGAC
TACTTGGCGTACCAGACCGACGACAAGCTGACCATCAGCGTCAAACCGCTTAGCGAATCCGACGCCGAGAAGCGCAAGGC
CGAGCACTTCACCTATTCCGGAGAGAAGCTGTCGCTGAATTTCCAGGATATCGACGTTCGCTCGGTTCTCCAGTTGATTG
CGGATTTCACCGATCTCAACTTGGTCGCCAGCGATACCGTGCAAGGCAACATCACGCTGCGTCTGCAGAACGTGCCGTGG
GATCAGGCGCTGGATCTGGTGCTGAAAACCAAAGGGCTCGACAAGCGTCAGGTTGGCAACGTGCTGCTGGTAGCGCCAGC
GGACGAGATCGCTGCACGTGAGCGTCAGGAGTTAGAGTCGCAGCGCCAGATCGCTGAACTGGCGCCTCTACGCCGTGAGG
TCGTGCAGGTCAATTATGCCAAAGCTTCTGATATTGCGCGGTTATTCCAGTCGGTTACCGACACGGACGGAGCCAGTGGG
GATCGCGGTTCGATCTCTGTTGACGATCGCACTAACAACATCATCGCCTACCAGACGCAGGAGCGGCTCGACGAGCTGCG
GCGCATCGTGGCGCAACTGGATATTCCCGTACGTCAGGTCATGATCGAGGCTCGCATAGTCGAGGCTAACGTTGATTATG
ACAAGAGCCTGGGTGTGCGTTGGGGGGGGAATCTCTGGGCCGGTGACAAATGGAATGCTTGGGGCAAAAACGGGCAGCTC
GGCGTAGCTGATGATCCTAATGCAAGCGGTAAACCGGGTGACGAGGGGTATATTCCTTCCCAGCAGGTCGGACGTTTCCC
CGGGATTCGTGAAGTACCTCTAAATACTCCTTTTGTTGACTTGGGCGCAGTCGGTGCAACCTCCGGGATTGGCTTGGGCT
TCGTCACCAATAATGCCATCCTTGATCTTCAACTCTCTGCCATGGAAAAAACAGGTAATGGAGAGGTTGTATCGCAACCG
AAGGTCGTTACCGCAGACAAGGAGACCGCAAAAATCTTAAAAGGCTCGGAAGTGCCCTATCAGGAGGCGAGTTCCAGTGG
CGCGACCAGCACTTCTTTCAAGGAGGCGGCGTTATCTTTGGAGGTCACTCCGCAAATCACTCCTGATAACCGCATCATCA
TGGAAGTGAAGGTCACCAAGGATGAGCCTGATTTCTCGCAAGCTGCCAGCACCGGTGGTATCCCTGCTATCCGTAAAAAC
GAGGTCAACGCAAAAGTCTTGGTCACTGATGGTGAGACGATCGTAATTGGCGGTGTGTTCTCTAATACTCAGAGCAAATC
CGTTGACAAGGTTCCGTTCTTAGGCGATCTGCCGTTCGTAGGCCGGATATTCCGTCGTGATTTGATTCAAGACCGGAAAT
CTGAGCTGCTGGTATTCATTACTCCTCGGATCATGAATAATCAGGCGATCCAAGCCAGCCGTTAG

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(300-346)

AMIN domain (AMIN)

(38-123)

Bacterial type II and III secretion system protein (Secretin)

(547-705)

Bacterial type II/III secretion system short domain (Secretin_N)

(373-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.69

100

0.738
  pilQ Acinetobacter baumannii strain A118

39.015

100

0.41
  pilQ Acinetobacter baumannii D1279779

39.015

100

0.41
  comQ Acinetobacter baylyi ADP1

40.954

99.86

0.409
  pilQ Legionella pneumophila strain ERS1305867

37.846

96.218

0.364
  pilQ Legionella pneumophila strain Lp02

37.7

96.218

0.363

Multiple sequence alignment