Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GYM54_RS11185 Genome accession   NZ_CP048411
Coordinates   2433458..2434522 (-) Length   354 a.a.
NCBI ID   WP_181104793.1    Uniprot ID   -
Organism   Pseudomonas sp. MTM4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2428458..2439522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYM54_RS11160 (GYM54_11145) aroK 2428993..2429538 (-) 546 WP_181104593.1 shikimate kinase AroK -
  GYM54_RS11165 (GYM54_11150) pilQ 2429546..2431690 (-) 2145 WP_181104595.1 type IV pilus secretin PilQ Machinery gene
  GYM54_RS11170 (GYM54_11155) pilP 2431742..2432269 (-) 528 WP_181104597.1 type 4a pilus biogenesis lipoprotein PilP -
  GYM54_RS11175 (GYM54_11160) pilO 2432266..2432889 (-) 624 WP_131651787.1 type 4a pilus biogenesis protein PilO -
  GYM54_RS11180 (GYM54_11165) pilN 2432886..2433458 (-) 573 WP_131651786.1 type 4a pilus biogenesis protein PilN -
  GYM54_RS11185 (GYM54_11170) pilM 2433458..2434522 (-) 1065 WP_181104793.1 pilus assembly protein PilM Machinery gene
  GYM54_RS11190 (GYM54_11175) - 2434707..2437151 (+) 2445 WP_197445225.1 penicillin-binding protein 1A -
  GYM54_RS11195 (GYM54_11180) - 2437309..2438577 (-) 1269 WP_131651783.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37854.25 Da        Isoelectric Point: 4.6313

>NTDB_id=421158 GYM54_RS11185 WP_181104793.1 2433458..2434522(-) (pilM) [Pseudomonas sp. MTM4]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYRVESYAVEPLPPNAVVEKNIAELEGVGQALTRLLAKAKTGVKSTAV
AVSGSAVITKSIEMEAGLSDDDLENQLKIEADQYIPYPLEEVAIDFEVQGAAPRSPGRVEVLLAACRKENVEIREAALAL
AGLTAKVVDVEAYALERSYNLLAPQLGAGHDELTVALVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSM
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFHDVDYILLAGGTASIPGLDRLIQQKIGTQTLVANP
FADMALSGKVNAGALASDAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=421158 GYM54_RS11185 WP_181104793.1 2433458..2434522(-) (pilM) [Pseudomonas sp. MTM4]
GTGCTAGGGCTCTTCACTAAGAAAGCGAACACGCTGCTTGGGATCGACATCAGTTCGACGTCCGTCAAGCTACTCGAATT
AAGCCGCTCAGGCTCCCGATATAGGGTTGAGTCCTATGCGGTCGAACCGCTTCCGCCAAATGCCGTGGTGGAAAAGAACA
TTGCTGAACTCGAAGGTGTAGGTCAGGCGCTGACTCGGTTGTTGGCCAAGGCCAAAACCGGAGTCAAGTCGACCGCCGTA
GCCGTTTCCGGCTCGGCAGTCATCACCAAGAGCATCGAGATGGAAGCCGGATTGTCTGATGACGATCTGGAAAACCAGTT
GAAGATCGAGGCGGACCAGTACATTCCTTATCCGCTGGAAGAGGTCGCGATCGATTTCGAAGTGCAGGGTGCTGCGCCTC
GTTCTCCCGGACGCGTCGAAGTGCTTCTGGCCGCGTGCCGCAAGGAGAACGTGGAGATTCGCGAGGCGGCGCTCGCCCTA
GCGGGGTTGACGGCCAAGGTCGTTGATGTCGAAGCCTATGCGCTGGAGCGTTCTTACAATCTATTGGCGCCGCAATTGGG
CGCTGGTCATGATGAGCTGACGGTGGCGCTCGTGGATATCGGCGCGACCATGACCACGCTGAGCGTGCTGCATAACGGTC
GCACCATCTATACGCGCGAACAGTTGTTCGGTGGCCGCCAGCTCACCGAGGAAATCCAGCGTCGCTACGGCCTTTCGATG
GAAGAGGCCGGTCTGGCCAAAAAGCAGGGAGGCCTTCCAGACGACTATGACAGCGAAGTGTTGCAGCCGTTCAAAGAGGC
GGTTGTTCAGCAGGTTTCACGCTCGCTGCAGTTCTTCTTCGCTGCGGGGCAGTTCCATGATGTGGACTACATCCTTTTGG
CGGGCGGTACGGCTTCAATCCCCGGGCTGGATCGGTTGATTCAGCAGAAAATCGGAACCCAGACTTTAGTCGCCAACCCT
TTTGCCGATATGGCTTTGAGCGGCAAGGTTAACGCGGGTGCACTGGCTAGTGATGCGCCATCGCTAATGATTGCCTGTGG
GTTGGCCTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

47.175

100

0.472

Multiple sequence alignment