Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GV127_RS05820 Genome accession   NZ_CP048402
Coordinates   1260559..1260993 (-) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain HS_Canada1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1255559..1265993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GV127_RS05790 (GV127_05790) - 1256123..1256392 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  GV127_RS05795 (GV127_05795) yacG 1256392..1256595 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  GV127_RS05800 (GV127_05800) coaE 1256606..1257226 (-) 621 WP_163884064.1 dephospho-CoA kinase -
  GV127_RS05805 (GV127_05805) - 1257241..1257963 (-) 723 WP_163884065.1 prepilin peptidase -
  GV127_RS05810 (GV127_05810) - 1257963..1259174 (-) 1212 WP_163884066.1 type II secretion system F family protein -
  GV127_RS05815 (GV127_05815) pilB 1259174..1260559 (-) 1386 WP_163884067.1 GspE/PulE family protein Machinery gene
  GV127_RS05820 (GV127_05820) pilA 1260559..1260993 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  GV127_RS05825 (GV127_05825) ampD 1261153..1261719 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GV127_RS05830 (GV127_05830) rppH 1262305..1262901 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  GV127_RS05835 (GV127_05835) - 1262904..1263698 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  GV127_RS05840 (GV127_05840) lgt 1263708..1264511 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  GV127_RS05845 (GV127_05845) - 1264517..1265368 (+) 852 WP_005751072.1 thymidylate synthase -
  GV127_RS05850 (GV127_05850) tadA 1265383..1265883 (+) 501 WP_163884068.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=421043 GV127_RS05820 WP_005741096.1 1260559..1260993(-) (pilA) [Pasteurella multocida strain HS_Canada1]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=421043 GV127_RS05820 WP_005741096.1 1260559..1260993(-) (pilA) [Pasteurella multocida strain HS_Canada1]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTGAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368

Multiple sequence alignment