Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GV127_RS05815 Genome accession   NZ_CP048402
Coordinates   1259174..1260559 (-) Length   461 a.a.
NCBI ID   WP_163884067.1    Uniprot ID   -
Organism   Pasteurella multocida strain HS_Canada1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1254174..1265559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GV127_RS05785 (GV127_05785) - 1254301..1255674 (-) 1374 WP_163884063.1 alanine/glycine:cation symporter family protein -
  GV127_RS05790 (GV127_05790) - 1256123..1256392 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  GV127_RS05795 (GV127_05795) yacG 1256392..1256595 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  GV127_RS05800 (GV127_05800) coaE 1256606..1257226 (-) 621 WP_163884064.1 dephospho-CoA kinase -
  GV127_RS05805 (GV127_05805) - 1257241..1257963 (-) 723 WP_163884065.1 prepilin peptidase -
  GV127_RS05810 (GV127_05810) - 1257963..1259174 (-) 1212 WP_163884066.1 type II secretion system F family protein -
  GV127_RS05815 (GV127_05815) pilB 1259174..1260559 (-) 1386 WP_163884067.1 GspE/PulE family protein Machinery gene
  GV127_RS05820 (GV127_05820) pilA 1260559..1260993 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  GV127_RS05825 (GV127_05825) ampD 1261153..1261719 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GV127_RS05830 (GV127_05830) rppH 1262305..1262901 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  GV127_RS05835 (GV127_05835) - 1262904..1263698 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  GV127_RS05840 (GV127_05840) lgt 1263708..1264511 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  GV127_RS05845 (GV127_05845) - 1264517..1265368 (+) 852 WP_005751072.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52213.97 Da        Isoelectric Point: 6.5002

>NTDB_id=421042 GV127_RS05815 WP_163884067.1 1259174..1260559(-) (pilB) [Pasteurella multocida strain HS_Canada1]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPPFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPMHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIVLTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=421042 GV127_RS05815 WP_163884067.1 1259174..1260559(-) (pilB) [Pasteurella multocida strain HS_Canada1]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGCGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGATATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAACTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTGAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTTCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTCCCTTTTCGGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGATGCATTTAGGCGAAAAAGCGGTATTACGTTTGCAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAACCTCAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGTGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTGCAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.991

98.265

0.668
  pilB Haemophilus influenzae Rd KW20

67.329

98.265

0.662
  pilB Glaesserella parasuis strain SC1401

58.37

98.482

0.575
  pilB Acinetobacter baumannii D1279779

39.798

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

39.545

100

0.414
  pilB Acinetobacter baylyi ADP1

37.924

100

0.412
  pilB Vibrio campbellii strain DS40M4

39.375

100

0.41
  pilB Vibrio cholerae strain A1552

38.604

100

0.408
  pilB Legionella pneumophila strain ERS1305867

37.475

100

0.399

Multiple sequence alignment