Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   M655_RS00935 Genome accession   NZ_CP048274
Coordinates   159065..159544 (+) Length   159 a.a.
NCBI ID   WP_007779761.1    Uniprot ID   -
Organism   Brevibacillus sp. NSP2.1     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 154837..181906 159065..159544 within 0


Gene organization within MGE regions


Location: 154837..181906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M655_RS00910 (M655_000900) - 154837..155217 (-) 381 WP_283858487.1 hypothetical protein -
  M655_RS00915 (M655_000905) - 155352..156502 (-) 1151 Protein_155 IS3 family transposase -
  M655_RS00920 (M655_000910) - 156636..157259 (-) 624 WP_026557677.1 SDR family oxidoreductase -
  M655_RS00925 (M655_000915) ychF 157505..158605 (+) 1101 WP_025846307.1 redox-regulated ATPase YchF -
  M655_RS00930 (M655_000920) rpsF 158748..159035 (+) 288 WP_005828370.1 30S ribosomal protein S6 -
  M655_RS00935 (M655_000925) ssbA 159065..159544 (+) 480 WP_007779761.1 single-stranded DNA-binding protein Machinery gene
  M655_RS00940 (M655_000930) rpsR 159586..159816 (+) 231 WP_005831740.1 30S ribosomal protein S18 -
  M655_RS00945 (M655_000935) - 159901..160809 (+) 909 WP_005831738.1 DUF2232 domain-containing protein -
  M655_RS00950 (M655_000940) - 160814..162751 (+) 1938 WP_007780468.1 DHH family phosphoesterase -
  M655_RS00955 (M655_000945) rplI 162778..163221 (+) 444 WP_025846303.1 50S ribosomal protein L9 -
  M655_RS00960 (M655_000950) dnaB 163246..164595 (+) 1350 WP_005831733.1 replicative DNA helicase -
  M655_RS00965 (M655_000955) - 164777..166063 (+) 1287 WP_005831731.1 adenylosuccinate synthase -
  M655_RS00970 (M655_000960) - 166228..166443 (+) 216 WP_081414729.1 hypothetical protein -
  M655_RS00975 (M655_000965) - 166519..166890 (-) 372 WP_005831726.1 hypothetical protein -
  M655_RS00980 (M655_000970) - 167148..168644 (+) 1497 WP_005831724.1 M23 family metallopeptidase -
  M655_RS00985 (M655_000975) yycF 168829..169539 (+) 711 WP_005831723.1 response regulator YycF -
  M655_RS00990 (M655_000980) walK 169539..171362 (+) 1824 WP_025846297.1 cell wall metabolism sensor histidine kinase WalK -
  M655_RS00995 (M655_000985) yycH 171359..172741 (+) 1383 WP_007780450.1 two-component system activity regulator YycH -
  M655_RS01000 (M655_000990) yycI 172728..173501 (+) 774 WP_005831717.1 two-component system regulatory protein YycI -
  M655_RS01005 (M655_000995) - 173545..174333 (+) 789 WP_007780446.1 MBL fold metallo-hydrolase -
  M655_RS01010 (M655_001000) - 174328..174564 (-) 237 WP_005831713.1 hypothetical protein -
  M655_RS01015 (M655_001005) - 174841..176061 (+) 1221 WP_005831709.1 S1C family serine protease -
  M655_RS01020 (M655_001010) - 176605..176847 (+) 243 WP_007780439.1 CxxH/CxxC protein -
  M655_RS01025 (M655_001015) rlmH 176854..177333 (+) 480 WP_007780437.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  M655_RS01030 (M655_001020) - 177518..178900 (+) 1383 WP_007780435.1 hypothetical protein -
  M655_RS01035 (M655_001025) - 179220..180343 (+) 1124 WP_157179509.1 IS3 family transposase -
  M655_RS01040 (M655_001030) - 180348..181268 (+) 921 WP_286086024.1 DUF1430 domain-containing protein -
  M655_RS01045 (M655_001035) - 181265..181906 (+) 642 WP_007780416.1 ABC transporter ATP-binding protein -

Sequence


Protein


Download         Length: 159 a.a.        Molecular weight: 17164.03 Da        Isoelectric Point: 5.2753

>NTDB_id=420486 M655_RS00935 WP_007779761.1 159065..159544(+) (ssbA) [Brevibacillus sp. NSP2.1]
MNKVILIGNLTKDPELRYTPNGVAVATFTVAVNRPRTNQAGEREADFINIVAWQKLADLCASYLRKGRQAAIEGRIQTRS
YDNKEGKRVYVTEVVAENVQFLGGRGNEGGGDNAGYDPGPGFGGGNKPSGQRNNDFDPFGDPFASAGKPINISDDDLPF

Nucleotide


Download         Length: 480 bp        

>NTDB_id=420486 M655_RS00935 WP_007779761.1 159065..159544(+) (ssbA) [Brevibacillus sp. NSP2.1]
ATGAATAAAGTTATTCTCATCGGCAACCTGACGAAAGATCCTGAACTTCGCTACACGCCGAATGGCGTTGCCGTTGCTAC
TTTTACCGTGGCTGTCAATCGTCCTCGCACCAATCAGGCAGGCGAGAGAGAAGCCGATTTCATTAATATTGTCGCTTGGC
AAAAACTTGCCGATCTTTGTGCAAGCTACTTGCGCAAAGGAAGACAAGCTGCCATCGAAGGACGCATTCAAACGCGCTCC
TACGATAATAAAGAAGGAAAACGCGTATATGTAACAGAGGTCGTTGCGGAGAACGTTCAATTTTTGGGCGGTCGAGGCAA
TGAAGGCGGCGGAGACAACGCAGGATACGATCCAGGACCAGGATTTGGTGGCGGAAACAAGCCATCCGGTCAGAGAAACA
ATGACTTTGATCCGTTTGGTGATCCCTTCGCGAGTGCAGGCAAGCCGATCAACATTTCTGATGACGACTTGCCGTTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

55.233

100

0.597

  ssb Latilactobacillus sakei subsp. sakei 23K

43.678

100

0.478

  ssb Vibrio cholerae strain A1552

36.757

100

0.428

  ssb Neisseria gonorrhoeae MS11

37.143

100

0.409

  ssb Neisseria meningitidis MC58

36.571

100

0.403

  ssb Glaesserella parasuis strain SC1401

34.463

100

0.384

  ssbB Bacillus subtilis subsp. subtilis str. 168

52.252

69.811

0.365


Multiple sequence alignment