Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GT972_RS13625 Genome accession   NZ_CP048030
Coordinates   2844841..2846586 (+) Length   581 a.a.
NCBI ID   WP_202922454.1    Uniprot ID   -
Organism   Sinimarinibacterium sp. NLF-5-8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2839841..2851586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GT972_RS13615 (GT972_13615) lptE 2841556..2842116 (-) 561 WP_238388270.1 LPS assembly lipoprotein LptE -
  GT972_RS13620 (GT972_13620) leuS 2842121..2844721 (-) 2601 WP_162079608.1 leucine--tRNA ligase -
  GT972_RS13625 (GT972_13625) pilB 2844841..2846586 (+) 1746 WP_202922454.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GT972_RS13630 (GT972_13630) pilC 2846634..2847863 (+) 1230 WP_162079093.1 type II secretion system F family protein Machinery gene
  GT972_RS13635 (GT972_13635) pilD 2847879..2848748 (+) 870 WP_162079094.1 A24 family peptidase Machinery gene
  GT972_RS15535 - 2849060..2849224 (-) 165 WP_238388271.1 hypothetical protein -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 62488.66 Da        Isoelectric Point: 5.1302

>NTDB_id=419612 GT972_RS13625 WP_202922454.1 2844841..2846586(+) (pilB) [Sinimarinibacterium sp. NLF-5-8]
MITPAQVQTPVFLGGLARRLVADGIVAEEVARAQQRKALAEGRPLVALLVESKAAAASRIAEAASLEYGAPLLDLNAVEM
DQAVAGELSEKVLRKAHALPLFRRDKKIFVAVSDPTNLAALDEIKFATGLQTEAIVVEEDKLTRLLEATIQAQSNAQMNL
GSDEGLENLDIGADDGGAESHGVDVTKSDADEAPIIRYVNKILVDAINQGASDVHFEPYEKTYRIRYRKDGELKVAATPP
VAQAQRLAARVKVMSRLDLAERRVPQDGRIKLYLSKTRAIDFRVSTCPTLFGEKIVCRILDPSSAKLGIDALGYDPVQKQ
AYLKALEQPQGMILVTGPTGSGKTVSLYTGLNILNTDDINISTAEDPAEINLPGINQVNVNPKAGLTFAAALRSFLRQDP
DVIMVGEIRDLETAEIAIKAAQTGHLVLSTLHTNDAPQTIVRLMNMGVPGYNLASTLTLIIAQRLARRLCPECKRPASIP
RAELLRNGLTETQIDAPDFRLMEAVGCDQCGGSGYKGRTGIYQVMPFSDAMGRIIMEGGNAIDIAEQAQKEGIQDLRQSA
LAKVAAGVIDLTQANAVTMGD

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=419612 GT972_RS13625 WP_202922454.1 2844841..2846586(+) (pilB) [Sinimarinibacterium sp. NLF-5-8]
ATGATTACTCCAGCACAAGTCCAAACGCCGGTGTTCCTGGGAGGACTGGCGCGCCGCTTGGTGGCCGACGGGATCGTTGC
CGAGGAGGTGGCGCGCGCGCAGCAGCGCAAGGCGCTGGCCGAGGGTCGCCCGCTGGTGGCGCTGCTGGTTGAAAGCAAGG
CGGCTGCGGCCAGCCGGATCGCCGAAGCGGCCAGTCTGGAATATGGCGCGCCGTTGCTGGATCTCAATGCCGTGGAAATG
GATCAGGCGGTGGCCGGAGAGCTCAGCGAAAAGGTTTTGCGCAAGGCGCATGCGCTGCCGTTGTTCCGGCGCGATAAAAA
AATCTTTGTGGCGGTTTCCGATCCGACCAATCTTGCGGCGCTGGACGAGATCAAGTTTGCAACCGGCTTGCAGACCGAGG
CGATCGTCGTCGAGGAGGACAAGCTGACCCGCTTGCTGGAGGCCACCATCCAGGCGCAGAGCAATGCGCAAATGAACCTG
GGCAGCGACGAAGGGCTGGAGAATCTGGATATCGGCGCCGATGACGGCGGCGCCGAAAGCCACGGTGTGGATGTCACCAA
AAGCGATGCCGACGAGGCGCCGATCATCCGCTACGTCAACAAGATTCTGGTCGATGCGATCAATCAGGGCGCATCCGACG
TGCACTTTGAGCCGTATGAAAAAACCTATCGCATCCGCTACCGCAAGGATGGCGAGCTCAAAGTGGCGGCGACGCCACCG
GTGGCGCAGGCACAGCGGCTGGCGGCGCGCGTCAAGGTGATGTCGCGGCTGGATCTGGCCGAACGGCGGGTGCCGCAGGA
CGGCCGCATCAAGCTGTATTTATCCAAAACCCGCGCGATCGATTTTCGTGTCAGCACCTGTCCGACGCTGTTTGGCGAAA
AAATCGTCTGCCGTATTCTCGATCCGTCCAGTGCCAAGCTGGGGATCGACGCGCTGGGCTACGACCCGGTGCAAAAACAG
GCGTATCTCAAGGCACTGGAACAGCCGCAGGGCATGATTCTGGTGACCGGCCCGACCGGCTCCGGCAAAACGGTGTCGCT
GTATACCGGCTTGAATATTCTCAACACCGACGACATCAACATCTCCACCGCCGAAGACCCGGCGGAAATCAACCTGCCCG
GCATCAATCAGGTCAACGTCAACCCCAAGGCCGGACTGACCTTTGCCGCTGCGCTGCGCTCGTTTTTGCGACAAGACCCC
GACGTCATCATGGTGGGCGAAATCCGCGATCTGGAAACCGCCGAAATTGCGATCAAGGCCGCACAGACCGGGCATCTGGT
GTTGTCCACGCTGCACACCAACGATGCGCCGCAAACCATCGTCCGACTGATGAACATGGGCGTGCCCGGCTATAACCTGG
CGTCCACGCTGACGCTGATCATTGCCCAGCGTCTGGCGCGACGGCTGTGTCCTGAATGCAAGCGCCCCGCCAGCATTCCG
CGCGCCGAGCTGTTGCGCAATGGGCTGACCGAAACGCAGATCGACGCGCCCGACTTCAGGCTCATGGAAGCCGTGGGCTG
CGATCAGTGCGGCGGCAGCGGCTACAAAGGCCGTACCGGGATTTATCAGGTGATGCCCTTTTCGGATGCGATGGGGCGCA
TCATCATGGAAGGCGGCAATGCCATCGACATTGCCGAGCAGGCGCAGAAAGAAGGCATCCAGGATTTGCGTCAGTCGGCG
CTGGCCAAGGTGGCGGCAGGGGTGATTGATCTGACCCAGGCCAATGCCGTGACGATGGGCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.912

98.107

0.539
  pilB Acinetobacter baylyi ADP1

54.912

98.107

0.539
  pilB Legionella pneumophila strain ERS1305867

53.846

98.451

0.53
  pilB Vibrio cholerae strain A1552

50.912

94.32

0.48
  pilF Neisseria gonorrhoeae MS11

49.117

97.418

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

50.185

93.287

0.468
  pilB Vibrio campbellii strain DS40M4

49.632

93.632

0.465
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.13

95.009

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

40.962

89.501

0.367

Multiple sequence alignment