Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H9W85_RS22690 Genome accession   NZ_CP060703
Coordinates   4910634..4912043 (-) Length   469 a.a.
NCBI ID   WP_031684900.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NRD619     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4905634..4917043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9W85_RS22680 (H9W85_22680) - 4907728..4908906 (-) 1179 WP_023116094.1 substrate-binding domain-containing protein -
  H9W85_RS22685 (H9W85_22685) pilC 4909420..4910634 (-) 1215 WP_031693843.1 type II secretion system inner membrane protein GspF Machinery gene
  H9W85_RS22690 (H9W85_22690) pilF 4910634..4912043 (-) 1410 WP_031684900.1 type II secretion system ATPase GspE Machinery gene
  H9W85_RS22695 (H9W85_22695) gspD 4912040..4914451 (-) 2412 WP_054375968.1 type II secretion system secretin GspD -
  H9W85_RS22700 (H9W85_22700) - 4914448..4915041 (-) 594 WP_003123065.1 type II secretion system protein M -
  H9W85_RS22705 (H9W85_22705) gspL 4915028..4916239 (-) 1212 WP_054375967.1 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 50977.38 Da        Isoelectric Point: 5.9527

>NTDB_id=416657 H9W85_RS22690 WP_031684900.1 4910634..4912043(-) (pilF) [Pseudomonas aeruginosa strain NRD619]
MSLLPYAWAKAQRALLRPGEHGATLLVSPRTPGWAISEVRQRHAPASLESVRDDELDTLLASAYSDTGSAAAVVGAAESE
VNLDRLMDDIPEVTDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFETHSVVRYRVDGALRDVVAPRKALHAALVSR
IKIMAQLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLRLETLGMAPGVLAPLDNLIRQPHG
IVLVTGPTGSGKTTTLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNARIDMTFAVALRAILRQDPDIIMIGEIRDL
ETAQIAVQASLTGHLVLATLHTNDAVSAVTRLVDMGVEPFLLASSMLGVLAQRLVRRLCTHCRVEEDGGWRAVGCPACNQ
TGYSGRTGIHELFVIDDEIRRLVHQGRAEQDLREAARAAGMRSMREDGERWIASGSTTLEEILRVTRDA

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=416657 H9W85_RS22690 WP_031684900.1 4910634..4912043(-) (pilF) [Pseudomonas aeruginosa strain NRD619]
ATGAGCCTGCTGCCCTACGCCTGGGCCAAGGCGCAGCGCGCCTTGCTGCGGCCGGGCGAGCATGGCGCGACCCTGCTGGT
CAGCCCGCGCACGCCCGGCTGGGCGATCAGCGAGGTGCGCCAGCGGCATGCGCCGGCCAGCCTGGAGAGCGTCCGCGACG
ACGAACTCGACACCCTGCTGGCCAGCGCCTACTCCGATACCGGCAGCGCCGCGGCGGTGGTCGGCGCGGCGGAGAGCGAG
GTCAACCTCGACCGCCTGATGGACGATATCCCCGAGGTCACCGATCTGCTCGACACCCAGGATGGCGCGCCGGTGATCCG
CATGATCAACGCGCTGCTGACCCAGGCCGCGCGCGATGAGGCCAGCGACATCCACATCGAGCCCTTCGAGACCCATTCGG
TGGTGCGCTATCGCGTCGACGGCGCCCTGCGCGACGTGGTCGCGCCGCGCAAGGCGCTGCACGCGGCGCTGGTGTCGCGG
ATCAAGATCATGGCGCAGCTGGATATCGCCGAGAAACGCCTGCCCCAGGATGGCCGCATCGCCTTGCGGGTAGCCGGGCG
GCCGATCGACATTCGCGTCTCCACCGTGCCCACCGGCCACGGCGAACGGGTGGTGATGCGCCTGCTGGACAAGCAGGCCG
GACGCCTGAGGCTGGAGACCCTGGGCATGGCTCCCGGCGTGCTGGCGCCGCTGGACAACCTGATCCGCCAGCCGCACGGC
ATCGTGCTGGTCACCGGGCCCACCGGCAGCGGCAAGACCACCACCCTCTACGCCGCCCTGGCGCGGCTCGACGCCAGCAC
CAGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGACCTGCCGGGGATCAGCCAGATCCAGGTCAACGCGCGCATCG
ACATGACCTTCGCCGTAGCCCTGCGGGCGATCCTCCGGCAGGACCCGGACATCATCATGATCGGCGAGATCCGCGACCTG
GAGACCGCGCAGATCGCCGTGCAGGCCTCGCTCACCGGGCACCTGGTGCTGGCCACGCTGCACACCAACGACGCGGTGTC
GGCAGTGACGCGGCTGGTGGACATGGGGGTCGAGCCGTTCCTGCTCGCCTCGTCGATGCTTGGCGTACTCGCCCAGCGCC
TGGTGCGGCGCCTGTGCACGCATTGCCGGGTCGAAGAGGACGGCGGCTGGCGCGCGGTCGGCTGCCCGGCCTGCAACCAG
ACCGGCTACAGCGGGCGCACCGGCATCCACGAACTGTTCGTGATCGACGACGAGATCCGCCGCCTGGTCCACCAGGGCCG
CGCCGAGCAGGATCTCCGCGAAGCCGCCCGCGCCGCCGGCATGCGCAGCATGCGCGAGGACGGCGAGCGCTGGATCGCCA
GCGGCTCCACCACCCTGGAGGAAATCCTCCGCGTGACCCGGGACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(111-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

50.928

80.384

0.409
  pilB/pilB1 Synechocystis sp. PCC 6803

41.113

99.574

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.462

83.156

0.403
  pilB Vibrio cholerae strain A1552

46.898

85.928

0.403
  pilB Vibrio campbellii strain DS40M4

45.61

87.42

0.399
  pilF Neisseria gonorrhoeae MS11

47.208

84.009

0.397
  pilB Vibrio parahaemolyticus RIMD 2210633

46.076

84.222

0.388
  pilB Legionella pneumophila strain ERS1305867

45.501

82.942

0.377
  pilB Acinetobacter baylyi ADP1

45.103

82.729

0.373
  pilB Acinetobacter baumannii D1279779

44.359

83.156

0.369
  ctsE Campylobacter jejuni subsp. jejuni 81-176

38.462

94.243

0.362