Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GVI47_RS26175 Genome accession   NZ_CP047698
Coordinates   5606137..5608224 (-) Length   695 a.a.
NCBI ID   WP_160288625.1    Uniprot ID   -
Organism   Pseudomonas knackmussii strain N1-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5601137..5613224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GVI47_RS26160 - 5602772..5604445 (-) 1674 WP_160288624.1 AAA family ATPase -
  GVI47_RS26165 aroB 5604452..5605558 (-) 1107 WP_043255932.1 3-dehydroquinate synthase -
  GVI47_RS26170 aroK 5605610..5606101 (-) 492 WP_408004252.1 shikimate kinase AroK -
  GVI47_RS26175 pilQ 5606137..5608224 (-) 2088 WP_160288625.1 type IV pilus secretin PilQ Machinery gene
  GVI47_RS26180 pilP 5608282..5608806 (-) 525 WP_043255936.1 type 4a pilus biogenesis lipoprotein PilP -
  GVI47_RS26185 pilO 5608803..5609483 (-) 681 WP_052355394.1 type 4a pilus biogenesis protein PilO -
  GVI47_RS26190 pilN 5609480..5610079 (-) 600 WP_043255938.1 type 4a pilus biogenesis protein PilN -
  GVI47_RS26195 pilM 5610079..5611143 (-) 1065 WP_043255941.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74958.99 Da        Isoelectric Point: 5.5504

>NTDB_id=415468 GVI47_RS26175 WP_160288625.1 5606137..5608224(-) (pilQ) [Pseudomonas knackmussii strain N1-2]
MNRTMTRLGVALLVGLFAPHLLAADLQSLDVASLPGDRVELKLGFDQPVVAPRGYTIEQPARIALDLPGVQNKLGSKNRE
LGVGNARSVTVVEAKDRTRLIINLGTLVPYSTRTQGNNLFILVGQGTGASAPVASATPAATSYASSARPAQPKPYIPAGK
AIRNIDFQRGDQGEGNVVIDLSDPGVSPDIQEQGGKIRLNFPKTQLPESLRVRLDVKDFATPVQFVNASEAGGNATINIE
PSGLFDYLVYQTDNRMTVSIKPLSAGDAEKRKKDTFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLR
LQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKASDIAKLFQSVTG
SATGRGASAATANTDNRGSITVDDRTNSIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEASVDYTKELGVNWSGSL
SNRGNWNVSGGTDSTSQTFVDLGVSTTTPTSGIGIGFVTNNTVLDLQLNAMEKTGNGEVVSQPKVVTSDKETAKILKGTE
VPYQEASSSGATSVSFKEAALSLEVTPQITPDNRIIMQVKVNKDEPDYQNALLGVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQTKTTAKVPFLGDVPVVGRLFKNDYVTDTKNELLVFLTPRIMNNQAIAIDR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=415468 GVI47_RS26175 WP_160288625.1 5606137..5608224(-) (pilQ) [Pseudomonas knackmussii strain N1-2]
ATGAACAGAACGATGACCCGCCTGGGCGTTGCCCTGCTGGTAGGCCTTTTTGCACCGCATCTGCTTGCGGCCGACCTGCA
GAGCCTTGACGTGGCCTCGCTGCCGGGCGATCGCGTGGAGCTCAAGCTCGGCTTCGACCAGCCTGTGGTGGCGCCGCGCG
GCTATACCATCGAGCAACCCGCGCGTATCGCGCTGGATCTGCCAGGTGTGCAGAACAAGCTGGGCAGCAAGAACCGCGAG
TTGGGCGTGGGTAACGCCCGCAGCGTGACGGTTGTCGAGGCCAAGGATCGAACCCGGTTGATCATCAACTTGGGCACGCT
GGTGCCGTATTCGACCCGGACCCAGGGCAACAATCTGTTCATTCTCGTCGGCCAGGGCACTGGAGCTTCCGCACCCGTTG
CCAGTGCAACGCCGGCGGCGACCAGCTATGCCTCTTCGGCTCGTCCGGCGCAGCCCAAGCCTTACATTCCGGCCGGCAAG
GCGATTCGCAACATCGACTTCCAGCGCGGTGATCAGGGTGAAGGCAATGTCGTCATCGACCTCTCCGATCCTGGTGTCAG
CCCGGACATTCAGGAGCAAGGCGGCAAGATTCGCCTGAATTTCCCCAAGACGCAGCTTCCTGAAAGCCTGCGTGTGCGCC
TGGACGTGAAGGACTTCGCCACTCCGGTGCAGTTCGTCAACGCTTCGGAAGCGGGTGGAAACGCCACCATCAACATCGAG
CCGAGCGGTCTGTTCGATTATCTGGTGTATCAGACCGACAACCGCATGACCGTGAGCATCAAACCGCTCAGCGCCGGTGA
TGCCGAGAAGCGCAAGAAGGACACATTCGCCTACACCGGCGAGAAGCTGTCGCTCAACTTCCAGGACATCGATGTACGCT
CGGTCCTGCAGCTCATTGCTGACTTCACCGATCTGAACCTGGTGGCTTCTGACACCGTGCAGGGCAACATCACCCTGCGC
CTGCAGAACGTGCCCTGGGACCAGGCGCTGGATCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGATTGGCAACGT
GCTTCTGGTGGCGCCGGCTGACGAGATCGCCGCTCGCGAGCGCCAGGAGCTGGAAGCGCAGAAACAGATTGCCGAACTGG
CGCCCCTGCGTCGTGAGTTGATCCAGGTGAACTACGCCAAGGCGTCTGATATTGCCAAGCTGTTCCAGTCGGTCACCGGT
AGCGCTACCGGTCGTGGTGCTTCGGCCGCGACCGCGAACACCGATAACCGCGGTTCGATCACCGTGGATGATCGCACCAA
CTCGATCATCGCCTACCAGACCCAGGACCGCCTGGACGAGCTGCGCCGTATCGTTTCGCAGCTCGACATCCCTGTGCGCC
AGGTGATGATCGAGGCTCGTATCGTCGAGGCGAGCGTCGATTACACCAAGGAGCTGGGTGTGAACTGGAGCGGCTCGCTG
AGCAATCGGGGCAACTGGAACGTTTCCGGTGGCACCGACAGCACCTCGCAAACCTTCGTTGACCTGGGCGTGAGCACTAC
TACTCCGACGTCCGGTATCGGCATCGGCTTCGTGACCAACAACACCGTTCTCGATCTTCAGCTCAACGCGATGGAAAAGA
CCGGTAATGGCGAAGTGGTATCGCAGCCCAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTGAAAGGCACCGAG
GTTCCGTACCAGGAAGCGAGCTCCAGCGGCGCGACCTCGGTTTCCTTCAAGGAGGCCGCACTGTCTCTGGAAGTCACTCC
GCAGATCACTCCTGACAACCGCATCATCATGCAGGTCAAGGTGAACAAGGACGAGCCGGACTACCAGAACGCGCTGCTCG
GTGTACCTCCGATCAAGAAGAACGAGGTCAACGCCAAGGTCCTGGTCAATGACGGCGAGACCATCGTTATCGGTGGTGTG
TTCTCCAACACTCAGACCAAGACGACCGCGAAGGTACCGTTCCTGGGTGATGTGCCGGTAGTCGGTCGCCTGTTCAAGAA
TGACTACGTGACGGACACCAAGAACGAGCTTCTGGTTTTCCTCACTCCCCGGATCATGAATAATCAGGCCATTGCTATCG
ATCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(306-352)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

AMIN domain (AMIN)

(40-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(380-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

76.105

100

0.793
  comQ Acinetobacter baylyi ADP1

42.052

99.568

0.419
  pilQ Acinetobacter baumannii D1279779

40.647

100

0.416
  pilQ Acinetobacter baumannii strain A118

40.647

100

0.416
  pilQ Legionella pneumophila strain Lp02

38.563

98.129

0.378
  pilQ Legionella pneumophila strain ERS1305867

38.563

98.129

0.378

Multiple sequence alignment