Detailed information NTG0415469

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: GVI47_RS26195
• Genome accession: NZ_CP047698
• Coordinates: 5610079..5611143 (-)
• Length: 354 a.a.
• NCBI ID: WP_043255941.1
• Uniprot ID: A0A024HQD8
• Organism: Pseudomonas knackmussii strain N1-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5605079..5616143

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GVI47_RS26170	aroK	5605610..5606101 (-)	492	WP_408004252.1	shikimate kinase AroK	-
GVI47_RS26175	pilQ	5606137..5608224 (-)	2088	WP_160288625.1	type IV pilus secretin PilQ	Machinery gene
GVI47_RS26180	pilP	5608282..5608806 (-)	525	WP_043255936.1	type 4a pilus biogenesis lipoprotein PilP	-
GVI47_RS26185	pilO	5608803..5609483 (-)	681	WP_052355394.1	type 4a pilus biogenesis protein PilO	-
GVI47_RS26190	pilN	5609480..5610079 (-)	600	WP_043255938.1	type 4a pilus biogenesis protein PilN	-
GVI47_RS26195	pilM	5610079..5611143 (-)	1065	WP_043255941.1	pilus assembly protein PilM	Machinery gene
GVI47_RS26200	-	5611329..5613770 (+)	2442	WP_160288626.1	penicillin-binding protein 1A	-
GVI47_RS26205	-	5613962..5614453 (-)	492	WP_043257828.1	YgjP-like metallopeptidase domain-containing protein	-
GVI47_RS26210	-	5614517..5615287 (-)	771	WP_160288627.1	class I SAM-dependent methyltransferase	-
GVI47_RS26215	-	5615329..5615754 (-)	426	WP_160288628.1	hypothetical protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37754.09 Da
• Isoelectric Point: 4.4567

>NTDB_id=415469 GVI47_RS26195 WP_043255941.1 5610079..5611143(-) (pilM) [Pseudomonas knackmussii strain N1-2]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGGRFKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVVAKARTGVKTAAV
AVAGSAVITKSIEMDAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGVSARNAERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAFGLLGDQLGGNADELTIAVVDIGATMTTLSVLHHGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYESEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMALSNKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=415469 GVI47_RS26195 WP_043255941.1 5610079..5611143(-) (pilM) [Pseudomonas knackmussii strain N1-2]
GTGCTAGGGCTCTTCAATAAGAAAGCGAACACACTGCTGGGGATCGACATCAGTTCGACTTCGGTCAAGCTCCTTGAGTT
GAGCCGCTCCGGTGGCCGCTTCAAGGTCGAGGCTTACGCGGTCGAGCCGCTGCCCCCGAACGCGGTGGTAGAAAAGAACA
TCGCCGAACTGGAGGGGGTCGGCCAGGCGCTGTCGCGCGTGGTCGCCAAGGCGCGCACCGGCGTGAAAACCGCCGCCGTC
GCCGTCGCCGGATCGGCGGTCATCACCAAATCCATCGAGATGGATGCTGGCCTCTCCGAGGACGAGCTGGAAAACCAGCT
CAAGATCGAGGCGGACCAGTACATCCCCTACCCCCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGGTATCGGCTC
GCAACGCTGAGCGCGTCGATGTGCTCCTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTGCGCGAAGCTGCGCTGGCGCTT
GCAGGGCTGACCGCCAAGGTGGTCGATGTCGAGGCCTACGCCCTGGAGCGCGCCTTCGGTCTGCTCGGTGACCAGTTGGG
CGGTAACGCCGATGAGCTGACGATCGCTGTGGTCGACATTGGCGCGACCATGACGACCCTGAGCGTGCTGCACCACGGCC
GCACTATCTATACCCGTGAGCAACTGTTCGGCGGGCGCCAACTTACCGAAGAGATCCAGCGTCGCTATGGTCTGTCGGTC
GAAGAGGCTGGCCTGGCGAAGAAACAGGGCGGGCTCCCGGACGACTACGAAAGCGAAGTGCTGCAGCCGTTCAAGGATGC
GGTGGTTCAGCAGGTGTCCCGTTCCCTGCAGTTCTTCTTCGCCGCAGGTCAGTTCAACGACGTCGATTACATCCTGCTCG
CCGGTGGCACGGCTTCGATCCCGGACCTGGATCGCCTGATCCAGCAGAAGATCGGCACGCCCACCCTGGTGGCCAACCCC
TTCGCCGACATGGCCCTGAGCAACAAGGTGAACGCCGGGGCGCTGGCCAGTGATGCGCCCGCGCTGATGATTGCCTGCGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A024HQD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.345	100	0.573
comM	Acinetobacter nosocomialis M2	57.345	100	0.573
comM	Acinetobacter baylyi ADP1	52.825	100	0.528
pilM	Legionella pneumophila strain ERS1305867	46.328	100	0.463