Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GT347_RS09165 Genome accession   NZ_CP047650
Coordinates   1995094..1996836 (+) Length   580 a.a.
NCBI ID   WP_160551660.1    Uniprot ID   A0A857J4J6
Organism   Xylophilus rhododendri strain KACC 21265     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1990094..2001836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GT347_RS09135 (GT347_09135) - 1990135..1990890 (-) 756 WP_229722808.1 EAL domain-containing protein -
  GT347_RS09140 (GT347_09140) - 1990988..1991962 (-) 975 WP_160551655.1 tripartite tricarboxylate transporter substrate binding protein -
  GT347_RS09145 (GT347_09145) - 1992077..1993060 (+) 984 WP_160551656.1 ABC transporter substrate-binding protein -
  GT347_RS09150 (GT347_09150) - 1993076..1993792 (+) 717 WP_160551657.1 MgtC/SapB family protein -
  GT347_RS09155 (GT347_09155) - 1993894..1994292 (+) 399 WP_160551658.1 virulence factor -
  GT347_RS09160 (GT347_09160) - 1994302..1994799 (-) 498 WP_160551659.1 Spy/CpxP family protein refolding chaperone -
  GT347_RS09165 (GT347_09165) pilB 1995094..1996836 (+) 1743 WP_160551660.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GT347_RS09170 (GT347_09170) pilC 1996880..1998097 (+) 1218 WP_160551661.1 type II secretion system F family protein Machinery gene
  GT347_RS09175 (GT347_09175) - 1998148..1999023 (-) 876 WP_160551662.1 Gfo/Idh/MocA family protein -
  GT347_RS09180 (GT347_09180) - 1999181..2000059 (+) 879 WP_195812407.1 A24 family peptidase -
  GT347_RS09185 (GT347_09185) coaE 2000076..2000675 (+) 600 WP_160551663.1 dephospho-CoA kinase -
  GT347_RS09190 (GT347_09190) zapD 2000684..2001439 (+) 756 WP_160555283.1 cell division protein ZapD -
  GT347_RS09195 (GT347_09195) - 2001468..2001689 (+) 222 WP_160551664.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62978.19 Da        Isoelectric Point: 5.4483

>NTDB_id=415012 GT347_RS09165 WP_160551660.1 1995094..1996836(+) (pilB) [Xylophilus rhododendri strain KACC 21265]
MAAIDALSKDSAPVALPGVGRTLIAAGLLSQKTAEDLVRKAAASRTNFIAELTGSGVVSAADLAHAVSAAFGAPLLDLSA
IDPLRLPKDLLDIKICHDYRVVVLSKRSNRLIVATADPSDQEAAEKIKFSTQLGVDWVIAEYDKLSKLLETTGTSASEQL
DNMVGSGGEFEFDEEPEALDPNATDNGNLAEVEDAPVVKFLHKMLIDAFNMRASDLHFEPYEHNYRVRFRVDGELREIAS
PPVAIKEKLASRIKVISRMDISEKRVPQDGKMKLKVGADRVIDFRVSTLPTLFGEKIVIRILDPSSARLGIEALGYEPEE
KERLLQAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNDRAGLTFAVALKSFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPVCKQPHE
VPRSALLDAGYTEEQLDGSWQPYRAVGCSACNNGYKGRVGIYQVMPVNEAMQEIILRDGSALEIAAQARRDGVRSLRESG
LHKVRLGMTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=415012 GT347_RS09165 WP_160551660.1 1995094..1996836(+) (pilB) [Xylophilus rhododendri strain KACC 21265]
ATGGCCGCGATTGACGCCCTCAGCAAAGACAGCGCCCCGGTCGCGCTTCCCGGCGTAGGCCGCACGCTGATCGCGGCTGG
CCTGCTGAGCCAGAAGACCGCCGAAGACCTGGTGCGCAAGGCGGCCGCGTCGCGCACCAACTTCATCGCCGAACTCACCG
GCTCGGGCGTGGTGTCCGCCGCCGACCTGGCCCATGCGGTGTCGGCAGCCTTCGGCGCACCGCTGCTCGATCTCTCGGCC
ATCGATCCGCTGCGCCTGCCCAAGGACCTGCTGGACATCAAGATCTGCCACGACTACCGCGTGGTCGTGCTCAGCAAGCG
CAGCAACCGCCTGATCGTGGCCACGGCCGATCCTTCGGACCAGGAAGCCGCCGAGAAGATCAAGTTCTCCACCCAGCTCG
GCGTGGACTGGGTCATCGCCGAATACGACAAGCTCTCCAAGCTGCTGGAGACCACCGGCACCTCGGCCTCCGAGCAGCTC
GACAACATGGTCGGCAGCGGCGGCGAATTCGAGTTCGACGAGGAGCCCGAGGCGCTCGACCCGAACGCCACCGACAACGG
CAACCTGGCCGAGGTGGAGGACGCGCCGGTCGTCAAGTTCCTGCACAAGATGCTGATCGACGCGTTCAACATGCGCGCCT
CCGACCTGCATTTCGAGCCCTACGAACACAACTACCGGGTGCGTTTCCGGGTCGACGGCGAGTTGCGCGAGATCGCCTCG
CCGCCGGTGGCGATCAAGGAGAAGCTGGCCTCGCGGATCAAGGTGATCTCGCGCATGGACATCTCCGAGAAACGGGTGCC
GCAGGACGGCAAGATGAAACTCAAGGTCGGTGCCGACCGGGTCATCGACTTCCGGGTGAGCACCCTGCCCACGCTCTTCG
GCGAGAAGATCGTGATCCGTATCCTGGACCCGAGCAGCGCCCGCCTGGGCATCGAGGCGCTCGGCTACGAGCCCGAGGAG
AAGGAGCGCCTGCTGCAGGCCATCGGCCGGCCCTACGGGATGATCCTGGTCACCGGGCCGACGGGCTCGGGCAAGACGGT
GTCGCTCTACACCTGCCTGAACCTGCTCAACAAGCCGGGCGTGAACATCGCCACGGCCGAGGACCCGTCGGAAATCAACC
TGCCGGGCGTCAACCAGGTCAACGTCAACGACCGTGCCGGGCTGACCTTCGCCGTCGCGCTCAAATCCTTCCTGCGCCAG
GATCCGGACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCGCAGACCGGCCA
TCTGGTGCTATCCACCCTGCACACCAACGACGCGCCGACCACACTCACGCGCATGCGCAACATGGGCATCGCGCCCTTCA
ACATCGCGTCCAGCGTGATCCTGATCACCGCGCAGCGCCTGGCGCGCCGGCTGTGCCCGGTCTGCAAGCAGCCGCACGAG
GTGCCGCGCTCGGCCCTGCTCGACGCCGGCTACACCGAAGAGCAGCTCGACGGCAGCTGGCAGCCCTACCGCGCGGTGGG
CTGTTCGGCCTGCAACAACGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCGGTGAACGAGGCCATGCAGGAGA
TCATCCTGCGCGACGGCAGCGCGCTCGAAATCGCCGCGCAGGCCAGGCGCGACGGCGTGCGTTCGCTGCGCGAATCCGGA
TTGCACAAGGTGCGCCTGGGCATGACCTCGCTAGAGGAAGTGCTGGCCGTGACCAATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A857J4J6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.701

100

0.538
  pilB Acinetobacter baumannii D1279779

53.86

98.276

0.529
  pilF Neisseria gonorrhoeae MS11

51.943

97.586

0.507
  pilB Legionella pneumophila strain ERS1305867

49.027

97.414

0.478
  pilB Vibrio cholerae strain A1552

45.878

96.207

0.441
  pilB Vibrio campbellii strain DS40M4

44.974

97.759

0.44
  pilB Vibrio parahaemolyticus RIMD 2210633

45.863

95.862

0.44
  pilF Thermus thermophilus HB27

38.621

100

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.5

97.931

0.367

Multiple sequence alignment