Detailed information NTG0414736

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: GU333_RS06695
• Genome accession: NZ_CP047630
• Coordinates: 1340551..1341402 (-)
• Length: 283 a.a.
• NCBI ID: WP_061774798.1
• Uniprot ID: A0A2A5SB33
• Organism: Lactococcus raffinolactis strain Lr_18_12S
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1335551..1346402

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GU333_RS06675 (GU333_06650)	purB	1336303..1337595 (-)	1293	WP_096039219.1	adenylosuccinate lyase	-
GU333_RS06680 (GU333_06655)	purK	1337626..1338690 (-)	1065	WP_167842230.1	5-(carboxyamino)imidazole ribonucleotide synthase	-
GU333_RS06685 (GU333_06660)	purE	1338677..1339171 (-)	495	WP_167842231.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
GU333_RS06690 (GU333_06665)	purD	1339164..1340417 (-)	1254	WP_167842232.1	phosphoribosylamine--glycine ligase	-
GU333_RS06695 (GU333_06670)	dprA	1340551..1341402 (-)	852	WP_061774798.1	DNA-processing protein DprA	Machinery gene
GU333_RS06700 (GU333_06675)	-	1341484..1342440 (-)	957	WP_167842233.1	ABC transporter permease	-
GU333_RS06705 (GU333_06680)	-	1342440..1343516 (-)	1077	WP_061774800.1	ABC transporter permease	-
GU333_RS06710 (GU333_06685)	-	1343519..1345033 (-)	1515	WP_061774801.1	ABC transporter ATP-binding protein	-
GU333_RS06715 (GU333_06690)	deoD	1345182..1345892 (-)	711	WP_061774802.1	purine-nucleoside phosphorylase	-

Sequence

Protein

• Length: 283 a.a.
• Molecular weight: 32073.10 Da
• Isoelectric Point: 8.4451

>NTDB_id=414736 GU333_RS06695 WP_061774798.1 1340551..1341402(-) (dprA) [Lactococcus raffinolactis strain Lr_18_12S]
MQNFDIYRLKKAGMSNLMVYQFLTLVADSQERNPLVCLPAFIRQVKMKQSAHFWEKFRSFDIKAIREEFQKFPSFSIQDK
VYPEALREIYNPPVLLFYQGDLSLLHQTKLGFVGSRDCSQVGVASVQKIIKELQDRFVIVSGLARGIDTASHIAAMKNGG
KTIAVIGTGLDVNYPRENYQLQAYIAKHQLLLSEYGPDEKPLKYHFPERNRIIAGLSRGVLVTEAKLRSGSLITCERAME
EGRDVFAVPGNIIDGVSDGCHHLIQEGAKLIFHGRDILMEYDM

Nucleotide

• Length: 852 bp

>NTDB_id=414736 GU333_RS06695 WP_061774798.1 1340551..1341402(-) (dprA) [Lactococcus raffinolactis strain Lr_18_12S]
ATGCAAAATTTCGATATCTATCGCTTAAAAAAAGCTGGCATGTCAAACTTAATGGTTTATCAGTTTTTAACCCTAGTTGC
AGACAGTCAAGAAAGAAACCCCTTGGTCTGTTTACCAGCCTTTATTCGACAAGTTAAGATGAAACAGTCAGCTCATTTTT
GGGAAAAATTCCGATCATTTGACATAAAAGCCATACGAGAGGAGTTTCAGAAATTTCCGTCTTTCTCAATTCAGGATAAG
GTCTATCCTGAAGCTTTGCGAGAAATTTATAATCCGCCCGTCTTACTTTTTTATCAGGGAGATCTGAGCTTGCTTCATCA
GACCAAACTGGGTTTTGTTGGCAGTCGAGATTGCAGTCAAGTAGGGGTTGCCAGTGTTCAGAAGATCATCAAAGAATTGC
AAGATCGCTTTGTGATTGTTAGTGGCTTAGCACGGGGCATTGATACAGCTAGTCATATTGCTGCTATGAAAAATGGTGGT
AAGACAATTGCTGTGATTGGAACAGGGTTAGATGTAAATTATCCACGAGAAAATTATCAACTGCAGGCTTATATCGCAAA
ACATCAGCTCCTTTTATCAGAATATGGGCCAGATGAAAAACCTTTGAAGTATCATTTTCCAGAGCGTAATCGAATTATTG
CAGGTTTATCTCGTGGTGTATTAGTGACAGAAGCCAAGCTCCGCTCAGGTAGTTTGATTACCTGTGAGCGGGCTATGGAA
GAGGGGAGAGATGTGTTTGCCGTTCCAGGTAATATCATAGATGGGGTGTCAGATGGCTGCCACCATTTGATTCAGGAAGG
TGCAAAACTGATTTTCCATGGTCGAGATATATTAATGGAGTACGACATGTGA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (75-271)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-64)

3D structure

• AlphaFold DB: A0A2A5SB33

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	62.898	100	0.629
dprA	Lactococcus lactis subsp. cremoris KW2	60.498	99.293	0.601
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	58.156	99.647	0.58
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	58.156	99.647	0.58
dprA/cilB/dalA	Streptococcus pneumoniae D39	58.156	99.647	0.58
dprA/cilB/dalA	Streptococcus pneumoniae R6	58.156	99.647	0.58
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	58.156	99.647	0.58
dprA/cilB/dalA	Streptococcus mitis SK321	57.801	99.647	0.576
dprA	Bacillus subtilis subsp. subtilis str. 168	49.321	78.092	0.385
dprA	Legionella pneumophila strain ERS1305867	40.23	92.226	0.371
dprA	Staphylococcus aureus MW2	35.664	100	0.36