Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   GU334_RS06795 Genome accession   NZ_CP047628
Coordinates   1393465..1394316 (-) Length   283 a.a.
NCBI ID   WP_061774798.1    Uniprot ID   A0A2A5SB33
Organism   Lactococcus raffinolactis strain Lr_19_14     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1388465..1399316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GU334_RS06775 (GU334_06740) purB 1389217..1390509 (-) 1293 WP_096039219.1 adenylosuccinate lyase -
  GU334_RS06780 (GU334_06745) purK 1390540..1391598 (-) 1059 WP_096824057.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  GU334_RS06785 (GU334_06750) purE 1391591..1392085 (-) 495 WP_096039221.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  GU334_RS06790 (GU334_06755) purD 1392078..1393331 (-) 1254 WP_061774797.1 phosphoribosylamine--glycine ligase -
  GU334_RS06795 (GU334_06760) dprA 1393465..1394316 (-) 852 WP_061774798.1 DNA-processing protein DprA Machinery gene
  GU334_RS06800 (GU334_06765) - 1394398..1395354 (-) 957 WP_167841391.1 ABC transporter permease -
  GU334_RS06805 (GU334_06770) - 1395354..1396430 (-) 1077 WP_061774800.1 ABC transporter permease -
  GU334_RS06810 (GU334_06775) - 1396433..1397947 (-) 1515 WP_096039222.1 ABC transporter ATP-binding protein -
  GU334_RS06815 (GU334_06780) deoD 1398096..1398806 (-) 711 WP_061774802.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 283 a.a.        Molecular weight: 32073.10 Da        Isoelectric Point: 8.4451

>NTDB_id=414691 GU334_RS06795 WP_061774798.1 1393465..1394316(-) (dprA) [Lactococcus raffinolactis strain Lr_19_14]
MQNFDIYRLKKAGMSNLMVYQFLTLVADSQERNPLVCLPAFIRQVKMKQSAHFWEKFRSFDIKAIREEFQKFPSFSIQDK
VYPEALREIYNPPVLLFYQGDLSLLHQTKLGFVGSRDCSQVGVASVQKIIKELQDRFVIVSGLARGIDTASHIAAMKNGG
KTIAVIGTGLDVNYPRENYQLQAYIAKHQLLLSEYGPDEKPLKYHFPERNRIIAGLSRGVLVTEAKLRSGSLITCERAME
EGRDVFAVPGNIIDGVSDGCHHLIQEGAKLIFHGRDILMEYDM

Nucleotide


Download         Length: 852 bp        

>NTDB_id=414691 GU334_RS06795 WP_061774798.1 1393465..1394316(-) (dprA) [Lactococcus raffinolactis strain Lr_19_14]
ATGCAAAATTTCGATATCTATCGCTTAAAAAAAGCTGGCATGTCAAACTTAATGGTTTATCAGTTTTTAACCCTAGTTGC
AGACAGTCAAGAAAGAAACCCCTTGGTCTGTTTACCAGCCTTTATTCGACAAGTTAAGATGAAACAGTCAGCTCATTTTT
GGGAAAAATTCCGATCATTTGACATAAAAGCCATACGAGAGGAGTTTCAGAAATTTCCGTCTTTCTCAATTCAGGATAAG
GTCTATCCTGAAGCTTTGCGAGAAATTTATAATCCGCCCGTCTTACTTTTTTATCAGGGAGATTTGAGCTTGCTTCATCA
GACCAAACTAGGTTTTGTTGGCAGTAGAGATTGCAGTCAAGTAGGGGTTGCCAGTGTTCAGAAGATCATCAAAGAATTGC
AAGATCGCTTTGTGATTGTTAGTGGCTTAGCACGGGGCATTGATACAGCTAGTCATATTGCTGCTATGAAAAATGGAGGT
AAGACAATTGCTGTGATTGGAACAGGGTTAGATGTGAATTATCCACGAGAAAATTATCAACTGCAGGCTTATATCGCAAA
ACATCAGCTCCTTTTATCAGAATATGGGCCAGATGAAAAACCTTTGAAGTATCATTTTCCAGAGCGTAATCGAATTATTG
CAGGTCTATCTCGTGGTGTATTAGTGACAGAAGCCAAGCTCCGCTCAGGTAGTTTGATTACCTGTGAGCGGGCTATGGAA
GAGGGGAGAGATGTGTTTGCCGTTCCAGGTAATATCATAGATGGGGTGTCAGATGGCTGCCACCATTTGATTCAGGAAGG
TGCAAAACTGATTTTCCATGGTCGAGATATATTAATGGAGTACGACATGTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-271)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(1-64)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A5SB33

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Streptococcus mutans UA159

62.898

100

0.629
  dprA Lactococcus lactis subsp. cremoris KW2

60.498

99.293

0.601
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

58.156

99.647

0.58
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

58.156

99.647

0.58
  dprA/cilB/dalA Streptococcus pneumoniae D39

58.156

99.647

0.58
  dprA/cilB/dalA Streptococcus pneumoniae R6

58.156

99.647

0.58
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

58.156

99.647

0.58
  dprA/cilB/dalA Streptococcus mitis SK321

57.801

99.647

0.576
  dprA Bacillus subtilis subsp. subtilis str. 168

49.321

78.092

0.385
  dprA Legionella pneumophila strain ERS1305867

40.23

92.226

0.371
  dprA Staphylococcus aureus MW2

35.664

100

0.36

Multiple sequence alignment