Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   GU336_RS05240 Genome accession   NZ_CP047616
Coordinates   1052765..1053616 (+) Length   283 a.a.
NCBI ID   WP_167838626.1    Uniprot ID   A0A6H0UBN0
Organism   Lactococcus raffinolactis strain Lr_19_5     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1047765..1058616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GU336_RS05220 (GU336_05210) deoD 1048275..1048985 (+) 711 WP_061774802.1 purine-nucleoside phosphorylase -
  GU336_RS05225 (GU336_05215) - 1049134..1050648 (+) 1515 WP_061774801.1 ABC transporter ATP-binding protein -
  GU336_RS05230 (GU336_05220) - 1050651..1051727 (+) 1077 WP_061774800.1 ABC transporter permease -
  GU336_RS05235 (GU336_05225) - 1051727..1052683 (+) 957 WP_061774799.1 ABC transporter permease -
  GU336_RS05240 (GU336_05230) dprA 1052765..1053616 (+) 852 WP_167838626.1 DNA-processing protein DprA Machinery gene
  GU336_RS05245 (GU336_05235) purD 1053750..1055003 (+) 1254 WP_167838627.1 phosphoribosylamine--glycine ligase -
  GU336_RS05250 (GU336_05240) purE 1054996..1055490 (+) 495 WP_096039221.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  GU336_RS05255 (GU336_05245) purK 1055483..1056541 (+) 1059 WP_167838628.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  GU336_RS05260 (GU336_05250) purB 1056572..1057864 (+) 1293 WP_167838629.1 adenylosuccinate lyase -

Sequence

Protein


Download         Length: 283 a.a.        Molecular weight: 32085.07 Da        Isoelectric Point: 8.1274

>NTDB_id=414649 GU336_RS05240 WP_167838626.1 1052765..1053616(+) (dprA) [Lactococcus raffinolactis strain Lr_19_5]
MQNFDIYRLKNAGMSNLMVYQFLTLVADSQERNPLVCLPAFIRQVKMKQSAHFWEKFRSFDIKAIREEFQKFPSFSIQDK
VYPEALREIYNPPVLLFYQGDLSLLHQTKLGFVGSRDCSQVGVASVQKIIKELQDRFVIVSGLARGIDTASHIAAMKNGG
KTIAVIGTGLDVNYPRENYQLQAYIAKHQLLLSEYGPDEKPLKYHFPERNRIIAGLSRGVLVTEAKLRSGSLITCERAME
EGRDVFAVPGNIIDGVSDGCHHLIQEGAKLIFYGRDILMEYDM

Nucleotide


Download         Length: 852 bp        

>NTDB_id=414649 GU336_RS05240 WP_167838626.1 1052765..1053616(+) (dprA) [Lactococcus raffinolactis strain Lr_19_5]
ATGCAAAATTTCGATATCTATCGCTTAAAAAATGCTGGCATGTCAAACTTAATGGTTTATCAGTTTTTAACCCTAGTTGC
AGACAGTCAAGAAAGAAACCCCTTGGTCTGTTTACCAGCCTTTATTCGACAAGTTAAGATGAAACAGTCAGCTCATTTTT
GGGAAAAATTCCGATCATTTGACATAAAAGCCATACGAGAGGAGTTTCAGAAATTTCCGTCTTTCTCAATTCAGGATAAG
GTCTATCCTGAAGCTTTGCGAGAAATTTATAATCCGCCCGTCTTACTTTTTTATCAGGGAGATTTGAGCTTGCTTCATCA
GACCAAACTGGGTTTTGTTGGCAGTCGAGATTGCAGTCAAGTAGGGGTTGCCAGTGTTCAGAAGATCATCAAAGAATTGC
AAGATCGCTTTGTGATTGTTAGTGGCTTAGCACGGGGCATTGATACAGCTAGTCATATTGCTGCTATGAAAAATGGAGGT
AAGACAATTGCTGTGATTGGAACAGGGTTAGATGTGAATTATCCACGAGAAAATTATCAACTGCAGGCTTATATCGCAAA
ACATCAGCTCCTTTTATCAGAATATGGGCCAGATGAAAAACCTTTGAAGTATCATTTTCCAGAGCGTAATCGAATTATTG
CAGGTCTATCTCGTGGTGTATTAGTGACAGAAGCCAAGCTCCGCTCAGGTAGTTTGATTACCTGTGAGCGGGCTATGGAA
GAGGGGAGAGATGTGTTTGCCGTTCCAGGTAATATCATAGATGGGGTGTCAGATGGCTGCCACCATTTGATTCAGGAAGG
TGCAAAACTGATTTTCTATGGTCGAGATATATTAATGGAGTACGACATGTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-271)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(1-64)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6H0UBN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Streptococcus mutans UA159

62.544

100

0.625
  dprA Lactococcus lactis subsp. cremoris KW2

60.142

99.293

0.597
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

57.801

99.647

0.576
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

57.801

99.647

0.576
  dprA/cilB/dalA Streptococcus pneumoniae D39

57.801

99.647

0.576
  dprA/cilB/dalA Streptococcus pneumoniae R6

57.801

99.647

0.576
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

57.801

99.647

0.576
  dprA/cilB/dalA Streptococcus mitis SK321

57.447

99.647

0.572
  dprA Bacillus subtilis subsp. subtilis str. 168

49.321

78.092

0.385
  dprA Legionella pneumophila strain ERS1305867

39.773

93.286

0.371
  dprA Staphylococcus aureus MW2

35.664

100

0.36

Multiple sequence alignment