Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   GU337_RS07075 Genome accession   NZ_CP047614
Coordinates   1393394..1394245 (-) Length   283 a.a.
NCBI ID   WP_061774798.1    Uniprot ID   A0A2A5SB33
Organism   Lactococcus raffinolactis strain Lr_19_7     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1388394..1399245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GU337_RS07055 (GU337_07015) purB 1389146..1390438 (-) 1293 WP_096039219.1 adenylosuccinate lyase -
  GU337_RS07060 (GU337_07020) purK 1390469..1391527 (-) 1059 WP_096824057.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  GU337_RS07065 (GU337_07025) purE 1391520..1392014 (-) 495 WP_096039221.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  GU337_RS07070 (GU337_07030) purD 1392007..1393260 (-) 1254 WP_061774797.1 phosphoribosylamine--glycine ligase -
  GU337_RS07075 (GU337_07035) dprA 1393394..1394245 (-) 852 WP_061774798.1 DNA-processing protein DprA Machinery gene
  GU337_RS07080 (GU337_07040) - 1394327..1395283 (-) 957 WP_061774799.1 ABC transporter permease -
  GU337_RS07085 (GU337_07045) - 1395283..1396359 (-) 1077 WP_061774800.1 ABC transporter permease -
  GU337_RS07090 (GU337_07050) - 1396362..1397876 (-) 1515 WP_167840075.1 ABC transporter ATP-binding protein -
  GU337_RS07095 (GU337_07055) deoD 1398025..1398735 (-) 711 WP_096039223.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 283 a.a.        Molecular weight: 32073.10 Da        Isoelectric Point: 8.4451

>NTDB_id=414600 GU337_RS07075 WP_061774798.1 1393394..1394245(-) (dprA) [Lactococcus raffinolactis strain Lr_19_7]
MQNFDIYRLKKAGMSNLMVYQFLTLVADSQERNPLVCLPAFIRQVKMKQSAHFWEKFRSFDIKAIREEFQKFPSFSIQDK
VYPEALREIYNPPVLLFYQGDLSLLHQTKLGFVGSRDCSQVGVASVQKIIKELQDRFVIVSGLARGIDTASHIAAMKNGG
KTIAVIGTGLDVNYPRENYQLQAYIAKHQLLLSEYGPDEKPLKYHFPERNRIIAGLSRGVLVTEAKLRSGSLITCERAME
EGRDVFAVPGNIIDGVSDGCHHLIQEGAKLIFHGRDILMEYDM

Nucleotide


Download         Length: 852 bp        

>NTDB_id=414600 GU337_RS07075 WP_061774798.1 1393394..1394245(-) (dprA) [Lactococcus raffinolactis strain Lr_19_7]
ATGCAAAATTTCGATATCTATCGCTTAAAAAAAGCTGGCATGTCAAACTTAATGGTTTATCAGTTTTTAACCCTAGTTGC
AGACAGTCAAGAAAGAAACCCCTTGGTCTGTTTACCAGCCTTTATTCGACAAGTTAAGATGAAACAGTCAGCTCATTTTT
GGGAAAAATTCCGATCATTTGACATAAAAGCCATACGAGAGGAGTTTCAGAAATTTCCGTCTTTCTCAATTCAGGATAAG
GTCTATCCTGAAGCTTTGCGAGAAATTTATAATCCGCCCGTCTTACTTTTTTATCAGGGAGATTTGAGCTTGCTTCATCA
GACCAAACTGGGTTTTGTTGGCAGTCGAGATTGCAGTCAAGTAGGGGTTGCCAGTGTTCAGAAGATCATCAAAGAATTGC
AAGATCGCTTTGTGATTGTTAGTGGCTTAGCACGGGGCATTGATACAGCTAGTCATATTGCTGCTATGAAAAATGGAGGT
AAGACAATTGCTGTGATTGGAACAGGGTTAGATGTGAATTATCCACGAGAAAATTATCAACTGCAGGCTTATATCGCAAA
ACATCAGCTCCTTTTATCAGAATATGGGCCAGATGAAAAACCTTTGAAGTATCATTTTCCAGAGCGTAATCGAATTATTG
CAGGTCTATCTCGTGGTGTATTAGTGACAGAAGCCAAGCTCCGCTCAGGTAGTTTGATTACCTGTGAGCGGGCTATGGAA
GAGGGGAGAGATGTGTTTGCCGTTCCAGGTAATATCATAGATGGGGTGTCAGATGGCTGCCACCATTTGATTCAGGAAGG
TGCAAAACTGATTTTCCATGGTCGAGATATATTAATGGAGTACGACATGTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-271)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(1-64)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A5SB33

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Streptococcus mutans UA159

62.898

100

0.629
  dprA Lactococcus lactis subsp. cremoris KW2

60.498

99.293

0.601
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

58.156

99.647

0.58
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

58.156

99.647

0.58
  dprA/cilB/dalA Streptococcus pneumoniae D39

58.156

99.647

0.58
  dprA/cilB/dalA Streptococcus pneumoniae R6

58.156

99.647

0.58
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

58.156

99.647

0.58
  dprA/cilB/dalA Streptococcus mitis SK321

57.801

99.647

0.576
  dprA Bacillus subtilis subsp. subtilis str. 168

49.321

78.092

0.385
  dprA Legionella pneumophila strain ERS1305867

40.23

92.226

0.371
  dprA Staphylococcus aureus MW2

35.664

100

0.36

Multiple sequence alignment