Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RTA_RS14920 Genome accession   NC_015677
Coordinates   3102305..3104044 (+) Length   579 a.a.
NCBI ID   WP_041675628.1    Uniprot ID   -
Organism   Ramlibacter tataouinensis TTB310     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3097305..3109044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RTA_RS14885 (Rta_29080) - 3097459..3097962 (+) 504 WP_013902242.1 CNP1-like family protein -
  RTA_RS14890 (Rta_29090) proB 3097973..3099127 (-) 1155 WP_049871389.1 glutamate 5-kinase -
  RTA_RS14895 (Rta_29100) cgtA 3099139..3100206 (-) 1068 WP_041675627.1 Obg family GTPase CgtA -
  RTA_RS14900 (Rta_29110) rpmA 3100280..3100540 (-) 261 WP_013902245.1 50S ribosomal protein L27 -
  RTA_RS14905 (Rta_29120) rplU 3100553..3100864 (-) 312 WP_013902246.1 50S ribosomal protein L21 -
  RTA_RS14910 (Rta_29130) - 3101116..3102045 (+) 930 WP_049871302.1 polyprenyl synthetase family protein -
  RTA_RS14920 (Rta_29140) pilB 3102305..3104044 (+) 1740 WP_041675628.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RTA_RS14925 (Rta_29150) pilC 3104082..3105299 (+) 1218 WP_013902249.1 type II secretion system F family protein Machinery gene
  RTA_RS14930 (Rta_29160) pilD 3105299..3106141 (+) 843 WP_013902250.1 A24 family peptidase Machinery gene
  RTA_RS14935 (Rta_29170) coaE 3106155..3106754 (+) 600 WP_013902251.1 dephospho-CoA kinase -
  RTA_RS14940 (Rta_29180) zapD 3106764..3107519 (+) 756 WP_013902252.1 cell division protein ZapD -
  RTA_RS14945 (Rta_29190) - 3107528..3107740 (+) 213 WP_041675629.1 DNA gyrase inhibitor YacG -
  RTA_RS14950 (Rta_29200) - 3107787..3108143 (-) 357 WP_013902254.1 hypothetical protein -
  RTA_RS14955 (Rta_29210) - 3108248..3108445 (-) 198 WP_013902255.1 DUF3606 domain-containing protein -
  RTA_RS20795 (Rta_29220) - 3108654..3108896 (+) 243 WP_143762984.1 hypothetical protein -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 63069.12 Da        Isoelectric Point: 5.4648

>NTDB_id=41351 RTA_RS14920 WP_041675628.1 3102305..3104044(+) (pilB) [Ramlibacter tataouinensis TTB310]
MAAVDPVAPEAASVALPGIARALVSARKLSQKSAEDLWRKAQANRSSFTAELTGSGAVSPFDLAHTMSAAFGAPMLDLDA
IDIKRLPRNLLDSKTCQAFRVVVLSKRNNRLIVATADPSDQQAAEKIKFATQMGVDWVIAEYDKLTKMVEANATTASETM
ESIVGDDFDFDESSMESSAADDEEKAGAAEVEDAPVVKFLHKMLLDAFTMRASDLHFEPYEHNYRVRFRVDGELREISSP
PVAIKDKLASRIKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARLGIEALGYEPQEK
ERLLEAVQRPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPSEINMPGVNQVNVNEKAGLTFAAALKSFLRQD
PDVIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCASCKAPADI
PHETLLDAGYKEEEVDGTWTPYRPVGCSACNNGYKGRVGIYQVMPISEEIQRIILRDGSSLEIAQQAESEGVRSLRQSGL
HKVKLGVTSLEEVLGCTNA

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=41351 RTA_RS14920 WP_041675628.1 3102305..3104044(+) (pilB) [Ramlibacter tataouinensis TTB310]
ATGGCCGCCGTCGATCCCGTCGCCCCCGAAGCCGCTTCCGTGGCCCTGCCCGGCATCGCACGTGCGCTGGTCTCGGCCCG
CAAGCTCAGCCAGAAATCGGCCGAGGACCTGTGGCGCAAGGCGCAGGCCAACCGCAGCAGCTTCACCGCCGAGCTCACCG
GCTCCGGCGCTGTCTCCCCCTTCGACCTGGCCCACACCATGTCGGCGGCTTTCGGCGCGCCGATGCTGGACCTGGACGCC
ATCGACATCAAGCGCCTGCCCCGCAACCTGCTGGACAGCAAGACCTGCCAGGCCTTCCGGGTGGTGGTGCTGAGCAAGCG
CAACAACCGGCTCATCGTCGCCACCGCCGACCCGTCGGACCAGCAGGCGGCCGAGAAGATCAAGTTCGCCACGCAGATGG
GCGTGGACTGGGTCATCGCCGAGTACGACAAGCTCACCAAGATGGTGGAGGCCAACGCCACCACCGCCAGCGAAACGATG
GAGAGCATCGTCGGTGACGACTTCGACTTCGACGAATCGTCGATGGAAAGCAGCGCCGCCGACGACGAGGAGAAGGCGGG
CGCCGCCGAGGTCGAGGACGCCCCGGTCGTCAAGTTCCTGCACAAGATGCTGCTAGACGCGTTCACCATGCGGGCCTCGG
ACCTGCACTTCGAGCCGTACGAGCACAACTACCGCGTGCGCTTCCGGGTAGACGGCGAGCTGCGCGAGATTTCCTCGCCC
CCGGTGGCCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTCATCTCGCGCATGGACATCTCCGAGAAGCGGGTGCCGCA
GGACGGGCGGATGAAGCTCAAGGTCGGACCCGACCGGGTGATCGACTTCCGCGTCAGCACGCTGCCCACGCTGTTCGGCG
AGAAGATCGTGATCCGTATCCTGGACCCGAGCAGCGCCAGGCTGGGCATCGAGGCGCTGGGCTACGAGCCTCAGGAGAAG
GAGCGGCTGCTCGAGGCCGTGCAGCGCCCGTACGGCATGGTGCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTC
GCTCTACACCTGCCTGAACCTGCTGAACAAGCCCGGCGTCAACATCTCCACGGCCGAGGACCCGTCCGAAATCAACATGC
CGGGCGTGAACCAGGTAAACGTCAACGAGAAGGCGGGCCTGACCTTCGCCGCCGCGCTCAAGTCCTTCCTGCGCCAGGAC
CCCGACGTCATCATGGTGGGCGAGATCCGCGACCTGGAGACGGCCGACATCGCCATCAAGGCGGCGCAGACCGGCCACCT
GGTGCTGTCCACGCTGCACACCAACGACGCGCCCACCACGCTCACGCGGATGCGCAACATGGGCATCGCGCCGTTCAACA
TCGCCTCCAGCGTCATCCTGATCACGGCGCAGCGCCTGGCGCGCCGCCTCTGTGCCAGCTGCAAGGCGCCGGCGGACATC
CCGCACGAGACCCTGCTGGACGCCGGCTACAAGGAAGAGGAAGTCGACGGCACCTGGACGCCCTACCGCCCGGTGGGCTG
CTCGGCGTGCAACAACGGCTACAAGGGGCGGGTCGGCATCTACCAGGTGATGCCCATCAGCGAAGAGATCCAGCGCATCA
TCCTGCGCGACGGCAGCTCGCTCGAGATCGCGCAGCAGGCCGAGAGCGAAGGCGTCCGGAGCCTGCGCCAGTCCGGGCTG
CACAAGGTCAAGCTGGGCGTCACCTCGCTGGAAGAAGTGCTCGGCTGCACCAACGCATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-149)

Type II/IV secretion system protein (T2SSE)

(202-469)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.296

96.2

0.532
  pilB Acinetobacter baylyi ADP1

52.847

97.064

0.513
  pilF Neisseria gonorrhoeae MS11

51.95

97.409

0.506
  pilB Legionella pneumophila strain ERS1305867

50.622

97.237

0.492
  pilB Vibrio campbellii strain DS40M4

45.438

96.546

0.439
  pilB Vibrio parahaemolyticus RIMD 2210633

46.211

93.437

0.432
  pilB Vibrio cholerae strain A1552

47.173

88.601

0.418
  pilF Thermus thermophilus HB27

38.869

94.646

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.212

92.055

0.361

Multiple sequence alignment