Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GTH07_RS02340 Genome accession   NZ_CP047305
Coordinates   485770..486240 (-) Length   156 a.a.
NCBI ID   WP_000649323.1    Uniprot ID   -
Organism   Vibrio cholerae O37 strain ATCC 25872     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 480770..491240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTH07_RS02320 (GTH07_02325) coaE 481244..481852 (-) 609 WP_000011559.1 dephospho-CoA kinase -
  GTH07_RS02325 (GTH07_02330) pilD 481849..482724 (-) 876 WP_000418755.1 A24 family peptidase Machinery gene
  GTH07_RS02330 (GTH07_02335) pilC 482782..484008 (-) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  GTH07_RS02335 (GTH07_02340) pilB 484051..485739 (-) 1689 WP_053048901.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GTH07_RS02340 (GTH07_02345) pilA 485770..486240 (-) 471 WP_000649323.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GTH07_RS02345 (GTH07_02350) nadC 486485..487375 (-) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GTH07_RS02350 (GTH07_02355) ampD 487671..488216 (+) 546 WP_000567316.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GTH07_RS02355 (GTH07_02360) fldB 488367..488885 (-) 519 WP_000690110.1 flavodoxin FldB -
  GTH07_RS02360 (GTH07_02365) xerD 489023..489931 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  GTH07_RS02365 (GTH07_02370) dsbC 489948..490700 (+) 753 WP_000115055.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 15828.93 Da        Isoelectric Point: 7.7816

>NTDB_id=412503 GTH07_RS02340 WP_000649323.1 485770..486240(-) (pilA) [Vibrio cholerae O37 strain ATCC 25872]
MKAYKNKLQQGFTLIELMIVVAVIGVLAAIAVPQYQNYVKKSEAAAAVATIRSLTTNIDTFMADTGNFPTDLTTLGAASN
MNKLGTITLGTNEVKLVFSNTDSAFASTDEVKMAKDATSGLWTCTVPTGVTLKGCTASGSGSGSGSGSGSGSGSGN

Nucleotide


Download         Length: 471 bp        

>NTDB_id=412503 GTH07_RS02340 WP_000649323.1 485770..486240(-) (pilA) [Vibrio cholerae O37 strain ATCC 25872]
ATGAAAGCGTATAAAAACAAACTACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTAGCAGTGATTGGTGTATT
GGCAGCGATTGCGGTTCCTCAATATCAAAACTACGTTAAAAAATCAGAAGCTGCAGCTGCAGTAGCAACAATACGCTCTT
TGACTACCAATATCGATACATTCATGGCTGATACCGGAAATTTTCCAACTGATTTAACTACATTGGGTGCAGCATCAAAT
ATGAATAAACTCGGTACGATTACACTTGGGACAAACGAAGTTAAATTAGTTTTCAGTAATACTGACTCCGCTTTTGCTTC
AACTGATGAAGTTAAAATGGCTAAAGATGCAACTTCTGGCCTTTGGACTTGTACTGTACCAACAGGTGTAACACTGAAAG
GATGTACAGCTTCTGGTTCTGGTTCTGGTTCTGGTTCTGGTTCTGGTTCTGGTTCTGGTTCTGGCAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-131)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

50

97.436

0.487
  pilA Vibrio cholerae strain A1552

50

97.436

0.487
  pilA Vibrio cholerae C6706

50

97.436

0.487
  pilA Vibrio parahaemolyticus RIMD 2210633

42.657

91.667

0.391
  pilA Pseudomonas aeruginosa PAK

42.963

86.538

0.372
  pilA Acinetobacter baumannii strain A118

39.86

91.667

0.365

Multiple sequence alignment