Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N026_RS01765 Genome accession   NZ_CP047267
Coordinates   359532..361637 (+) Length   701 a.a.
NCBI ID   WP_024660872.1    Uniprot ID   -
Organism   Pseudomonas syringae UB303     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 354532..366637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N026_RS01745 (N026_01745) comM 356701..357765 (+) 1065 WP_003403040.1 pilus assembly protein PilM Machinery gene
  N026_RS01750 (N026_01750) - 357765..358340 (+) 576 WP_003403038.1 PilN domain-containing protein -
  N026_RS01755 (N026_01755) pilO 358337..358960 (+) 624 WP_024660871.1 type 4a pilus biogenesis protein PilO -
  N026_RS01760 (N026_01760) pilP 358957..359484 (+) 528 WP_011266376.1 type 4a pilus biogenesis lipoprotein PilP -
  N026_RS01765 (N026_01765) pilQ 359532..361637 (+) 2106 WP_024660872.1 type IV pilus secretin PilQ Machinery gene
  N026_RS01770 (N026_01770) aroK 361642..362160 (+) 519 WP_003403034.1 shikimate kinase AroK -
  N026_RS01775 (N026_01775) aroB 362275..363378 (+) 1104 WP_003403032.1 3-dehydroquinate synthase -
  N026_RS01780 (N026_01780) - 363389..365011 (+) 1623 WP_011266379.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75361.71 Da        Isoelectric Point: 5.0294

>NTDB_id=411943 N026_RS01765 WP_024660872.1 359532..361637(+) (pilQ) [Pseudomonas syringae UB303]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTSQPARIALDLPGVTSQLATKSRD
LGTGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAGAAQASQPTTAMGAQRVAAPPPTPASARPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPGIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGSG
KWTTYGLDNNGDEAGNTGSSVTSNVPFVDLGAADATTGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=411943 N026_RS01765 WP_024660872.1 359532..361637(+) (pilQ) [Pseudomonas syringae UB303]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCGGGTGACCGCATCGAGCTGAAGCTGGCATTCGACGCCCCGGTGCCTGCACCGCGCG
GTTACACCACCTCGCAACCGGCGCGCATTGCGCTGGACCTGCCGGGTGTTACCAGCCAGCTGGCGACCAAAAGTCGTGAC
CTGGGCACCGGCAATGCGCGCAGTGTGGTCGTGGTACAGGCTCAGGACCGTACCCGGGTGATCATCAACCTGACCACGCT
TTCGCCGTACAGCTCCCGCGTCGATGGCAACAACCTGTTCGTGGTCATCGGCCAGGGTGCCGGCGCCGCGCAAGCCTCCC
AGCCGACTACCGCGATGGGCGCACAACGGGTCGCAGCGCCACCCCCCACGCCGGCATCTGCCAGGCCGTTCATCCCCGCT
GGCGTCAAGGCCATCAGGAATGTCGACTTCCAGCGTGGCGAGCTGGGTGAGGGCAATGTGGTCATCGACCTGAGCAACCC
CGGCATTGCCCCGGATATTCAGGAGCAGGGCGGCAAGATCCGCGTGGACTTCGCCAAGACCCAGCTGCCCGAACCGCTGC
GCGTCCGCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAGTGCTTCGGCGACAGGTGACAAGGCCAGCATT
CTGATCGAGCCGTCGGGCGCCTTCGACTACTCGGCGTTCCAGACCGAAAACAAGCTGACGATCAGCGTGCGCCCGTTGAC
CAACGAGGATCTGGACCGCCGCGCTGCCGACAAGCCGGTGTACACCGGTGAAAAGCTGTCGCTGAACTTCCAGGACATCG
ATGTGCGTTCGGTGCTGCAGCTGATTGCCGACTTCACCAACCTCAATCTGGTGGCCAGTGATACGGTCCAGGGCGGTATC
ACCCTGCGTCTGCAGAATGTGCCATGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAGGGCCTGGACAAGCGCAAGGT
CGGCAACGTTCTACTGGTCGCACCGGCTGATGAAATTGCCGCCCGCGAGCGTCAGGAGCTGGAGTCACTCAAACAGATCG
CCGAGCTGGCACCCCTGCGCCGTGAGCTGCTGCAGGTCAATTATGCCAAGGCTGCCGACATCGCCAAGCTGTTCCAGTCC
GTGACCAGTGCCGAGTCCAAGGCCGACGAGCGTGGCTCGATCACGGTGGACGACCGGACCAACAACATCATTGCCTACCA
GACCCAGGAACGACTCGACGAACTGCGTCGTATCGTGTCGCAACTGGACATCCCGGTACGCCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAGCTCGGGGTACGCTGGGGCGGGTCTACCAACACCAGCGGCAGCGGC
AAATGGACAACCTACGGGCTGGATAATAACGGCGACGAGGCCGGTAACACTGGCAGCAGTGTGACCTCCAACGTGCCGTT
CGTGGACCTGGGCGCTGCTGATGCGACGACCGGCATAGGCCTGGGCTTCGTGACCAACAACACGCTGCTGGACCTTGAGC
TGAGTGCCATGGAGAAAACCGGTAACGGTGAAATCGTCTCCCAGCCCAAGGTGGTCACCTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGCACGGAGATCCCGTATCAGGAATCCAGCTCCAGCGGTGCGACCACCGTGAGCTTCAAGGAAGCTTCCCT
GTCGCTTGAAGTGACGCCGCAGATCACGCCGGACAACCGCATCATCATGGAAGTGAAGGTCACCAAGGACGAGCCGGACT
ACCTGAATGCGGTACTGGGCGTACCGCCCATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGATTTCCGACGGCGAAACC
ATCGTCATCGGTGGTGTGTTCTCGAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(536-692)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.232

100

0.753
  comQ Acinetobacter baylyi ADP1

42.241

99.287

0.419
  pilQ Acinetobacter baumannii D1279779

38.336

100

0.401
  pilQ Acinetobacter baumannii strain A118

38.336

100

0.401
  pilQ Legionella pneumophila strain Lp02

38.551

98.431

0.379
  pilQ Legionella pneumophila strain ERS1305867

38.551

98.431

0.379

Multiple sequence alignment