Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   N026_RS01745 Genome accession   NZ_CP047267
Coordinates   356701..357765 (+) Length   354 a.a.
NCBI ID   WP_003403040.1    Uniprot ID   A0AAJ4E2D4
Organism   Pseudomonas syringae UB303     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 351701..362765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N026_RS01730 (N026_01730) - 352530..353798 (+) 1269 WP_024650770.1 malic enzyme-like NAD(P)-binding protein -
  N026_RS01740 (N026_01740) - 354050..356491 (-) 2442 WP_370661161.1 penicillin-binding protein 1A -
  N026_RS01745 (N026_01745) comM 356701..357765 (+) 1065 WP_003403040.1 pilus assembly protein PilM Machinery gene
  N026_RS01750 (N026_01750) - 357765..358340 (+) 576 WP_003403038.1 PilN domain-containing protein -
  N026_RS01755 (N026_01755) pilO 358337..358960 (+) 624 WP_024660871.1 type 4a pilus biogenesis protein PilO -
  N026_RS01760 (N026_01760) pilP 358957..359484 (+) 528 WP_011266376.1 type 4a pilus biogenesis lipoprotein PilP -
  N026_RS01765 (N026_01765) pilQ 359532..361637 (+) 2106 WP_024660872.1 type IV pilus secretin PilQ Machinery gene
  N026_RS01770 (N026_01770) aroK 361642..362160 (+) 519 WP_003403034.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38093.54 Da        Isoelectric Point: 4.7049

>NTDB_id=411942 N026_RS01745 WP_003403040.1 356701..357765(+) (comM) [Pseudomonas syringae UB303]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVKNVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=411942 N026_RS01745 WP_003403040.1 356701..357765(+) (comM) [Pseudomonas syringae UB303]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCGTTGCCGGCCAACGCTGTCGTCGAAAAGAACA
TTGCCGAACTCGAAGGGGTCGGGCAGGCGTTGTCTCGCGTGCTCGCCAAGGCCAAGACCAGCGTCAAGAACGTGGCGGTT
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGATGACATGGAAAACCAGCT
CAAGCTTGAAGCTGACCAGTACATCCCCTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTATTCGGTGC
GCAATCCCGAACGCGTCGAGGTGTTGCTGGCTGCCTGCCGCAAGGAGAACGTCGAAGTGCGCGAGGCAGCCCTGGCGCTG
GCCGGGCTGACCGCGCGCGTGGTCGATGTCGAGGCCTACGCCCTCGAGCGCTCGTTCGGACTGCTGGCCGCGCAACTGGG
CAACGGTCATGACGAGCTGACGGTGGCCGTGGTCGATATAGGCGCCACCATGACCACCCTGAGCGTGCTGCATCATGGCC
GGATCATTTATACCCGCGAGCAGCTGTTTGGCGGGCGCCAGCTGACTGACGAAATTCAGCGCCGCTACGGGCTCTCCATG
GAGGAAGCCGGGCTTGCCAAGAAGCAGGGCGGCTTGCCCGACGACTACGTCAGCGAGGTGCTCGACCCCTTCAAGGAAGC
GCTGGTGCAGCAGGTTTCCCGCTCCCTGCAGTTCTTCTTTGCGGCCGGTCAATACAACTCCGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATCTGATCCAGCGGCGCATCGGCACTCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGTTCCAAGGTCAATGCCGGCGCCCTGGCCAGTGATGCGCCGGCGCTGATGATCGCGTGTGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

46.761

100

0.469

Multiple sequence alignment