Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N015_RS24980 Genome accession   NZ_CP047265
Coordinates   5574450..5576549 (-) Length   699 a.a.
NCBI ID   WP_024689066.1    Uniprot ID   A0AAU8LMW3
Organism   Pseudomonas asturiensis strain CC1524     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5569450..5581549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N015_RS24965 (N015_24965) - 5571055..5572686 (-) 1632 WP_024689068.1 SPOR domain-containing protein -
  N015_RS24970 (N015_24970) aroB 5572697..5573800 (-) 1104 WP_024689067.1 3-dehydroquinate synthase -
  N015_RS24975 (N015_24975) aroK 5573927..5574445 (-) 519 WP_004882714.1 shikimate kinase AroK -
  N015_RS24980 (N015_24980) pilQ 5574450..5576549 (-) 2100 WP_024689066.1 type IV pilus secretin PilQ Machinery gene
  N015_RS24985 (N015_24985) - 5576597..5577124 (-) 528 WP_024689065.1 pilus assembly protein PilP -
  N015_RS24990 (N015_24990) pilO 5577121..5577744 (-) 624 WP_024689064.1 type 4a pilus biogenesis protein PilO -
  N015_RS24995 (N015_24995) - 5577741..5578313 (-) 573 WP_024689063.1 PilN domain-containing protein -
  N015_RS25000 (N015_25000) pilM 5578313..5579377 (-) 1065 WP_024689062.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75430.81 Da        Isoelectric Point: 4.9933

>NTDB_id=411934 N015_RS24980 WP_024689066.1 5574450..5576549(-) (pilQ) [Pseudomonas asturiensis strain CC1524]
MTRILSIIGVSLWIAMMSPVLQAANLKTLDVAALPGDRIELKLAFDSPVPAPRGYTTEQPARIALDLPGVTSQLATKSRD
LGAGNARSVVVVQAQDRTRVIINLTSLSPYSSRVEGNNLFVVIGQGAAAAQASQPSTAKGAVRVAVPPPTPASARPYVPA
GARAIRNVDFQRGELGEGNVIIDLSNASIAPDIQEQGGKIRVDFAKTQLPDPLRVRLDVKDFATPVQFVSATASGDKASI
SIEPSGAFDYSAFQTENKLTISVRPLTNEDVDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAGDIAKLFQS
VTSAESKTDERGSITVDERTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKQIGVRWGGRTDRSRKW
SIGGLDDNGDEAGNTGNDLTANIPFVDLGAPDATAGVGIGFLTNNALLDLELSAMEKTGNGEIVSQPKVVTSDKETAKIL
KGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGETIV
IGGVFSNTQSKVVEKVPFLGDVPYLGRLFRRDVVSEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=411934 N015_RS24980 WP_024689066.1 5574450..5576549(-) (pilQ) [Pseudomonas asturiensis strain CC1524]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGGATAGCGATGATGTCGCCGGTTCTCCAGGCGGCCAACCTCAA
GACCCTGGACGTTGCTGCGCTGCCGGGTGACCGGATCGAGCTCAAGCTTGCATTCGATTCGCCGGTGCCAGCCCCGCGTG
GCTACACGACCGAGCAGCCGGCGCGCATTGCGCTGGACCTGCCAGGCGTGACCAGTCAGCTTGCAACCAAAAGCCGTGAT
CTGGGTGCGGGCAACGCACGCAGCGTGGTCGTGGTTCAGGCACAGGATCGTACGCGGGTCATCATCAACCTGACGTCGCT
GTCGCCGTACAGCTCGCGGGTCGAAGGCAATAATCTGTTCGTGGTGATCGGTCAGGGTGCCGCTGCCGCCCAGGCCTCGC
AGCCCAGTACCGCCAAGGGCGCTGTCAGGGTTGCAGTACCACCGCCCACGCCAGCCTCTGCGCGGCCTTATGTGCCTGCC
GGTGCGCGGGCCATTCGCAACGTCGACTTCCAGCGCGGTGAGCTGGGCGAAGGCAATGTGATCATCGACTTGAGTAACGC
CAGCATTGCGCCGGACATTCAGGAGCAGGGCGGCAAGATCCGTGTGGACTTCGCCAAGACCCAGTTGCCCGATCCACTGC
GTGTGCGCCTGGACGTCAAGGATTTCGCCACCCCGGTGCAGTTCGTGAGCGCCACCGCCTCGGGCGACAAGGCCAGCATC
TCCATCGAGCCGTCCGGTGCGTTCGACTATTCGGCCTTCCAGACCGAAAACAAGCTGACCATCAGTGTGCGTCCATTGAC
CAATGAGGACGTGGACCGACGTGCCGCCGACAAGCCGGTGTATACCGGCGAAAAGCTGTCACTCAACTTTCAGGACATCG
ACGTGCGTTCGGTGCTGCAGTTGATCGCCGATTTCACCAACCTCAATCTGGTTGCCAGTGACACGGTTCAGGGCGGCATT
ACCCTGCGACTGCAGAACGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTCAAGACCAAAGGGCTGGACAAGCGCAAGGT
CGGTAGCGTGTTGCTGGTCGCGCCCGCCGACGAGATTGCAGCCCGTGAGCGTCAGGAACTGGAATCGCTCAAGCAGATTG
CCGAGCTGGCACCGCTACGCCGCGAACTGCTGCAGGTCAACTACGCCAAGGCAGGCGACATCGCCAAGCTGTTTCAGTCG
GTCACCAGTGCCGAGTCCAAGACCGACGAGCGCGGTTCCATCACCGTGGATGAGCGTACCAACAACATCATTGCCTACCA
GACTCAGGACCGTCTGGACGAGTTGCGCCGCATTGTCTCGCAACTCGACATTCCGGTACGTCAGGTCATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGACTATGACAAGCAGATCGGCGTGCGCTGGGGCGGCCGGACCGATCGGTCTCGCAAGTGG
AGCATTGGCGGTCTCGATGATAACGGCGATGAAGCAGGCAATACGGGGAACGACCTGACCGCGAATATTCCTTTCGTGGA
TCTGGGGGCACCCGATGCCACGGCTGGCGTTGGCATCGGCTTTCTGACCAATAACGCACTGCTTGACCTTGAACTCAGTG
CGATGGAAAAAACCGGTAACGGCGAAATCGTCTCCCAGCCCAAGGTCGTGACCTCTGACAAGGAAACCGCCAAGATCCTC
AAGGGCACGGAGATTCCGTATCAGGAATCCAGCTCGAGCGGTGCCACGACCGTGAGCTTCAAGGAAGCTTCCCTGTCGCT
GGAAGTCACCCCGCAGATCACCCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCCGACTACCTCA
ATGCTGTGCTGGGTGTGCCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGATTTCCGACGGCGAGACGATCGTG
ATTGGTGGTGTGTTCTCCAATACGCAAAGTAAAGTTGTAGAGAAAGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCG
CCTTTTCAGGCGCGACGTGGTATCAGAGGCAAAATCCGAGCTGTTGGTATTTCTGACACCGCGTATCATGAACAACCAGG
CGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(534-690)

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.261

100

0.745
  comQ Acinetobacter baylyi ADP1

40.656

100

0.408
  pilQ Acinetobacter baumannii strain A118

37.671

100

0.393
  pilQ Acinetobacter baumannii D1279779

37.671

100

0.393
  pilQ Legionella pneumophila strain ERS1305867

38.067

97.711

0.372
  pilQ Legionella pneumophila strain Lp02

38.067

97.711

0.372

Multiple sequence alignment