Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GSF12_RS10390 Genome accession   NZ_CP047226
Coordinates   2275999..2277705 (-) Length   568 a.a.
NCBI ID   WP_159375396.1    Uniprot ID   -
Organism   Moraxella osloensis strain YV1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2270999..2282705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSF12_RS10370 (GSF12_10370) - 2271721..2273127 (+) 1407 WP_159375393.1 nicotinate phosphoribosyltransferase -
  GSF12_RS10375 (GSF12_10375) coaE 2273185..2273841 (-) 657 WP_228274246.1 dephospho-CoA kinase -
  GSF12_RS10380 (GSF12_10380) pilD 2273838..2274713 (-) 876 WP_159375394.1 prepilin peptidase Machinery gene
  GSF12_RS10385 (GSF12_10385) pilC 2274719..2275942 (-) 1224 WP_159375395.1 type II secretion system F family protein Machinery gene
  GSF12_RS10390 (GSF12_10390) pilB 2275999..2277705 (-) 1707 WP_159375396.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GSF12_RS10395 (GSF12_10395) tpiA 2278129..2278983 (+) 855 WP_159375397.1 triose-phosphate isomerase -
  GSF12_RS10400 (GSF12_10400) secG 2279075..2279395 (+) 321 WP_036594931.1 preprotein translocase subunit SecG -
  GSF12_RS10415 (GSF12_10415) rimP 2279956..2280465 (+) 510 WP_007115081.1 ribosome maturation factor RimP -
  GSF12_RS10420 (GSF12_10420) nusA 2280582..2282063 (+) 1482 WP_065264333.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62764.27 Da        Isoelectric Point: 5.1538

>NTDB_id=411615 GSF12_RS10390 WP_159375396.1 2275999..2277705(-) (pilB) [Moraxella osloensis strain YV1]
MAASLPSFGGIASRLVHEGLISEEAMQKVLAESQQQKVNLIAYVVDNKLAQADAVAWVISREFGDPLIDLDAINVNYIPK
EAVDEKIIREFNILPIFQRGSRLFVAMSDPTWVDALDALRFGTRFTVETVVAEHHKIKSLIEKIFTTTNLADFDDMEALE
FDETRADEDSSATSHLDINDEAPVVKFVNEMLIKAITMGASDLHFEPYEKSYRVRFRIDGVLQKMANPPTQLAGKIAARL
KVMSQMDISERRVPQDGRIKLKISKDKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQKQLFMDALAKPQG
MLLITGPTGSGKTVSLYTGLNILNTVETNISTAEDPVEINLEGINQVNVNNKVGLTFASALKSFLRQDPDIVMVGEIRDL
ETAEIAVKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKSCKKPTDVPKNSLLEMGFTE
EDLADPSNVIYEPVGCAECRDGYKGRVGIYEVMKVTPEIARIIMEDGNAIEIKGAALKQGFRDLRRSGILKVLKGTTSIQ
EMYRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=411615 GSF12_RS10390 WP_159375396.1 2275999..2277705(-) (pilB) [Moraxella osloensis strain YV1]
ATGGCAGCATCCCTTCCTAGTTTTGGTGGCATTGCCTCGCGATTAGTGCATGAAGGACTAATTAGCGAAGAGGCAATGCA
AAAAGTATTAGCAGAATCGCAGCAGCAAAAAGTAAATTTAATCGCCTATGTGGTTGACAATAAGCTTGCGCAAGCTGACG
CGGTAGCGTGGGTAATTTCGCGAGAATTTGGCGACCCACTCATTGATTTGGATGCCATCAATGTCAACTATATTCCAAAA
GAAGCGGTTGATGAAAAAATTATTCGCGAATTTAATATTCTGCCGATTTTCCAACGAGGTAGCCGCTTATTTGTTGCCAT
GAGCGACCCAACGTGGGTAGATGCTTTAGACGCGCTGCGGTTTGGTACAAGATTTACCGTAGAAACAGTGGTTGCCGAAC
ACCACAAAATCAAATCACTCATCGAAAAAATCTTTACTACCACCAATTTAGCTGATTTTGATGATATGGAAGCGTTGGAG
TTTGATGAAACCCGCGCCGATGAAGACAGTAGTGCCACCAGTCATTTAGATATCAATGATGAAGCGCCTGTCGTCAAGTT
CGTTAATGAGATGCTCATCAAAGCCATTACCATGGGCGCATCAGACTTACATTTTGAACCCTATGAAAAATCTTATCGCG
TGCGTTTTCGTATTGATGGCGTGTTACAAAAAATGGCAAATCCACCGACGCAGCTAGCAGGTAAAATTGCTGCCCGCCTG
AAAGTTATGTCACAAATGGACATCTCTGAACGCCGCGTGCCGCAAGATGGTCGTATCAAATTAAAAATTTCCAAGGATAA
GGCGATTGATTTTCGTGTCAACTCATTGCCAACCTTATTTGGTGAAAAAATCGTACTGCGTATTTTAGACCCATCATCAG
CGATGCTAGGTATTGATGCACTCGGCTATGAGCCGGACCAAAAGCAGCTATTTATGGATGCGCTGGCAAAACCGCAAGGG
ATGTTATTGATTACCGGCCCTACTGGTTCGGGTAAAACCGTCTCCTTGTATACGGGTCTTAATATCTTAAACACAGTTGA
GACCAATATCTCCACCGCAGAAGATCCCGTTGAAATTAACCTTGAAGGCATCAATCAGGTCAATGTGAACAATAAAGTTG
GCTTGACCTTTGCCAGTGCGCTCAAATCATTCTTGCGTCAAGACCCTGATATTGTAATGGTCGGTGAGATTCGTGACTTG
GAAACCGCTGAAATTGCTGTCAAAGCTGCCCAAACAGGGCATATGGTACTGTCAACCTTGCATACCAACTCAGCGCCAGA
AACCTTAACGCGTCTGCGGAATATGGGTGTGGCATCTTTTAACATTGCCACCTCAGTCAATTTGGTAATCGCACAGCGCT
TAGCACGTCGCCTATGTAAATCCTGCAAAAAACCGACAGATGTACCAAAAAATAGCTTGCTTGAAATGGGCTTTACTGAA
GAAGATTTGGCAGATCCCAGCAATGTCATTTATGAGCCCGTTGGCTGCGCTGAATGCCGTGATGGCTATAAAGGACGTGT
TGGTATTTATGAAGTGATGAAAGTAACACCAGAGATTGCCCGAATTATTATGGAAGACGGCAACGCTATTGAGATTAAAG
GCGCTGCTTTAAAGCAAGGGTTTAGGGATTTAAGGCGCTCAGGTATTTTAAAGGTTCTAAAAGGCACGACCAGTATCCAA
GAGATGTATCGTGTTACTAGCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

67.668

99.648

0.674
  pilB Acinetobacter baylyi ADP1

65.603

99.296

0.651
  pilB Legionella pneumophila strain ERS1305867

51.59

99.648

0.514
  pilF Neisseria gonorrhoeae MS11

49.374

98.415

0.486
  pilB Vibrio cholerae strain A1552

49.005

97.359

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

46.975

98.944

0.465
  pilB Vibrio campbellii strain DS40M4

46.333

98.415

0.456
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.229

100

0.393
  pilF Thermus thermophilus HB27

36.602

100

0.368

Multiple sequence alignment