Detailed information NTG0411613

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GSF12_RS10380
• Genome accession: NZ_CP047226
• Coordinates: 2273838..2274713 (-)
• Length: 291 a.a.
• NCBI ID: WP_159375394.1
• Uniprot ID: A0A6P1KP71
• Organism: Moraxella osloensis strain YV1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2268838..2279713

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GSF12_RS10360 (GSF12_10360)	nfuA	2269633..2270250 (-)	618	WP_159375392.1	Fe-S biogenesis protein NfuA	-
GSF12_RS10365 (GSF12_10365)	-	2270549..2271652 (+)	1104	WP_228274245.1	bifunctional nicotinamide-nucleotide adenylyltransferase/Nudix hydroxylase	-
GSF12_RS10370 (GSF12_10370)	-	2271721..2273127 (+)	1407	WP_159375393.1	nicotinate phosphoribosyltransferase	-
GSF12_RS10375 (GSF12_10375)	coaE	2273185..2273841 (-)	657	WP_228274246.1	dephospho-CoA kinase	-
GSF12_RS10380 (GSF12_10380)	pilD	2273838..2274713 (-)	876	WP_159375394.1	prepilin peptidase	Machinery gene
GSF12_RS10385 (GSF12_10385)	pilC	2274719..2275942 (-)	1224	WP_159375395.1	type II secretion system F family protein	Machinery gene
GSF12_RS10390 (GSF12_10390)	pilB	2275999..2277705 (-)	1707	WP_159375396.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GSF12_RS10395 (GSF12_10395)	tpiA	2278129..2278983 (+)	855	WP_159375397.1	triose-phosphate isomerase	-
GSF12_RS10400 (GSF12_10400)	secG	2279075..2279395 (+)	321	WP_036594931.1	preprotein translocase subunit SecG	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32215.67 Da
• Isoelectric Point: 8.1651

>NTDB_id=411613 GSF12_RS10380 WP_159375394.1 2273838..2274713(-) (pilD) [Moraxella osloensis strain YV1]
MGIFSDFILLLQQSQWLTLIIVALLGLCVGSFLNVVIYRTPLMMEQQWKNECQLLLHPETVIDNHESMTLSRPASRCPHC
GHPIRWFENMPVISWLLLKGKCSQCHNAISYRYPLVEIITAILSVLVVMKFGVTWQALAGLALTWTLVALTGIDFDTQLL
PDRFTLPLAGLGLFVNAFGLFVSPTAAIFGYVFGFLCLWIVYQLFLLITGKHGMGHGDFKLLAALGAWLGPLMLPLIVLL
SAFVGSVVGVILMKRSGESKPFAFGPYIAIAGMIALLYGQSIMSWYLGKMS

Nucleotide

• Length: 876 bp

>NTDB_id=411613 GSF12_RS10380 WP_159375394.1 2273838..2274713(-) (pilD) [Moraxella osloensis strain YV1]
ATGGGGATTTTTTCAGATTTTATTTTGTTACTACAACAGTCACAATGGCTGACGTTGATTATCGTAGCTTTATTGGGCTT
GTGTGTGGGTAGTTTTCTCAATGTGGTGATTTATCGCACGCCGTTAATGATGGAGCAGCAGTGGAAGAACGAATGCCAGC
TATTGCTGCATCCAGAAACCGTTATTGATAATCATGAATCCATGACCTTAAGCCGTCCTGCCTCTCGTTGCCCGCATTGT
GGGCATCCAATACGTTGGTTTGAAAATATGCCTGTTATTAGCTGGCTACTGCTAAAAGGAAAATGTAGTCAGTGTCATAA
CGCCATTAGTTATCGTTATCCGCTTGTAGAAATCATCACCGCTATTTTGTCTGTGTTGGTGGTGATGAAATTTGGTGTTA
CTTGGCAAGCGCTGGCAGGATTAGCACTCACATGGACATTAGTGGCTTTGACAGGCATTGATTTTGATACCCAATTGTTG
CCTGACCGATTTACATTGCCGTTAGCGGGATTAGGATTATTCGTCAATGCCTTTGGCTTGTTTGTCAGTCCCACAGCGGC
TATTTTTGGCTATGTGTTTGGGTTTTTATGCCTGTGGATTGTCTATCAACTGTTTTTGCTGATTACGGGCAAGCACGGGA
TGGGGCATGGCGATTTTAAATTATTAGCGGCTTTGGGGGCTTGGCTAGGGCCTTTGATGCTACCCTTAATTGTGCTTTTA
TCTGCGTTTGTCGGCTCGGTTGTGGGTGTAATATTGATGAAGCGCTCAGGTGAGAGTAAACCTTTTGCATTTGGACCCTA
TATCGCCATTGCTGGCATGATAGCACTTTTGTATGGGCAATCAATAATGTCGTGGTATCTAGGCAAAATGTCATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (24-131)

• Type IV leader peptidase family (Peptidase_A24) (142-250)

3D structure

• AlphaFold DB: A0A6P1KP71

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter nosocomialis M2	63.38	97.595	0.619
pilD	Acinetobacter baumannii D1279779	63.38	97.595	0.619
pilD	Vibrio campbellii strain DS40M4	48.399	96.564	0.467
pilD	Vibrio cholerae strain A1552	49.27	94.158	0.464
pilD	Neisseria gonorrhoeae MS11	45.556	92.784	0.423