Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GRF56_RS21455 Genome accession   NZ_CP047155
Coordinates   4660113..4661819 (+) Length   568 a.a.
NCBI ID   WP_005336134.1    Uniprot ID   -
Organism   Aeromonas veronii strain AVNIH1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4655113..4666819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRF56_RS21430 (GRF56_21430) pdhR 4656282..4657046 (-) 765 WP_159144446.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  GRF56_RS21435 (GRF56_21435) ampD 4657325..4657900 (-) 576 WP_069554218.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GRF56_RS21440 (GRF56_21440) - 4658031..4658501 (+) 471 WP_040068932.1 retropepsin-like aspartic protease family protein -
  GRF56_RS21445 (GRF56_21445) nadC 4658505..4659380 (+) 876 WP_042053539.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GRF56_RS22550 (GRF56_21450) - 4659696..4660109 (+) 414 WP_074042658.1 pilin -
  GRF56_RS21455 (GRF56_21455) pilB 4660113..4661819 (+) 1707 WP_005336134.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  GRF56_RS21460 (GRF56_21460) pilC 4662060..4663301 (+) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  GRF56_RS21465 (GRF56_21465) pilD 4663363..4664235 (+) 873 WP_064341795.1 prepilin peptidase Machinery gene
  GRF56_RS21470 (GRF56_21470) coaE 4664249..4664863 (+) 615 WP_042053536.1 dephospho-CoA kinase -
  GRF56_RS21475 (GRF56_21475) zapD 4664901..4665623 (+) 723 WP_201296125.1 cell division protein ZapD -
  GRF56_RS21480 (GRF56_21480) yacG 4665633..4665827 (+) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  GRF56_RS21485 (GRF56_21485) mutT 4665905..4666309 (-) 405 WP_019444787.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62533.87 Da        Isoelectric Point: 5.3192

>NTDB_id=411098 GRF56_RS21455 WP_005336134.1 4660113..4661819(+) (pilB) [Aeromonas veronii strain AVNIH1]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=411098 GRF56_RS21455 WP_005336134.1 4660113..4661819(+) (pilB) [Aeromonas veronii strain AVNIH1]
ATGACCTCTAGCCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGACTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGTCTTCATACCGAAGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAATGACGCCCTCGGCATGGAGGATATCGACGAATCCGAGATT
TCGGATCTCGAGGTTTCCGACGAGAACAGCCGGCTGGACGAGAGTGTCAATACCGCCGATGACGATGCTCCCATCGTCAA
GTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGCGCCTCAGACCTGCACTTTGAGCCCTATGAGTTCAAGTATC
GGGTACGCTTTCGCATCGACGGCATTCTGCATGAAATTGCCACCCCGCCGGTCAATCTCTCCAACCGCTTCTCCGCTCGC
CTCAAGGTCATGGCACGACTCGATATCGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGTTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGCCAGAAGGCCCAATACCTGCGCGCCCTCTCCAAGCCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAATATCTCTACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGATCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
CTCGAAACCGCCGAGATCGCTATCAAGGCGGCGCAGACCGGTCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCCTCGGTCACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAAGCGCCGGAAGTGGTGCCGGAAGCAGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGTTGGCGGCAGGGCTTCGACTGTTCAAACCGGTCGGCTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGCTGATGTCGGAGAACATCGCCAAATTGATCATGCAAGGGGCCAACTCCCTGCAAATCGCCG
CTATCGCGCAGAAGGAGGGGATGCGCACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAATCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377

Multiple sequence alignment