Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GQR90_RS00890 Genome accession   NZ_CP047025
Coordinates   191039..192193 (+) Length   384 a.a.
NCBI ID   WP_158772504.1    Uniprot ID   -
Organism   Cobetia sp. L2A1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 186039..197193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQR90_RS00870 - 187287..187868 (-) 582 WP_158772500.1 YggT family protein -
  GQR90_RS00875 proC 188004..188846 (-) 843 WP_158772501.1 pyrroline-5-carboxylate reductase -
  GQR90_RS00880 - 188956..189693 (-) 738 WP_158772502.1 YggS family pyridoxal phosphate-dependent enzyme -
  GQR90_RS00885 pilT 189875..190906 (+) 1032 WP_158772503.1 type IV pilus twitching motility protein PilT Machinery gene
  GQR90_RS00890 pilU 191039..192193 (+) 1155 WP_158772504.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GQR90_RS00895 - 192328..193476 (-) 1149 WP_158772505.1 anhydro-N-acetylmuramic acid kinase -
  GQR90_RS00900 tyrS 193620..194828 (+) 1209 WP_158772506.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 42987.34 Da        Isoelectric Point: 6.1500

>NTDB_id=409957 GQR90_RS00890 WP_158772504.1 191039..192193(+) (pilU) [Cobetia sp. L2A1]
MTPHEWLQELLQLMVSKGSSDLFISTGTPPQMKVNGRMIALGDKKLDVDQVRELVLAPMSDMQRTRFEEEREANFAHSLA
GVGRFRISAFFQRSQMGMVIRRIQLTIPSLEELRLPDIIKGLSETKRGLVIFVGGTGAGKSTSLAAMIQHRNQTSSGHII
CIEDPIEYIHPHQRSIVTQREVGIDTESFEVALRNTLRQAPDVIMIGEIRSRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPEERHEQVWMDLSLNLKGIVAQQLLPHKSGDGAQRVPAIEVMLRSPLIVDLIRKGAVVEIKDVMKRSQQLGM
MTFDKSLYALHQQGLITEEVALAHADSANDLRLMIKFGDSDSAQEAQLDVLNAASRFSLQDDDD

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=409957 GQR90_RS00890 WP_158772504.1 191039..192193(+) (pilU) [Cobetia sp. L2A1]
ATGACTCCTCATGAATGGCTGCAGGAACTCCTGCAGTTGATGGTCAGCAAGGGTAGCTCCGACCTTTTCATCTCTACCGG
CACGCCGCCTCAGATGAAGGTCAATGGGCGCATGATCGCACTGGGCGACAAGAAGCTTGATGTTGATCAGGTGCGAGAGC
TGGTGCTTGCTCCCATGAGCGATATGCAGCGCACACGATTTGAAGAAGAGCGCGAAGCCAATTTTGCGCACAGCCTGGCA
GGGGTCGGGCGCTTCAGAATCAGTGCCTTCTTTCAGCGTAGCCAGATGGGCATGGTGATTCGTCGTATCCAGTTGACGAT
TCCGAGTCTGGAAGAATTGCGGCTGCCCGACATCATCAAGGGGCTTTCCGAGACCAAGCGCGGATTGGTGATCTTTGTCG
GCGGTACTGGTGCCGGTAAGTCCACCTCACTGGCAGCGATGATTCAGCATCGAAATCAAACTTCCAGCGGGCACATCATT
TGTATCGAAGATCCGATTGAGTACATCCACCCTCATCAGCGCTCCATTGTGACGCAGCGCGAAGTGGGCATTGATACTGA
GTCCTTTGAGGTGGCGCTGCGCAATACTCTTCGCCAGGCGCCCGACGTGATCATGATTGGTGAGATTCGTTCACGCGAGA
CCATGGAGCACGCATTGACCTTCGCCGAGACAGGTCACCTGTGTCTGGCGACGTTGCATGCCAATAATGCGAATCAGGCA
TTGGACCGCATTATTCACTTCTTCCCCGAGGAGCGTCATGAGCAAGTCTGGATGGATCTGTCGCTGAATCTAAAGGGGAT
TGTGGCGCAGCAGTTGCTGCCGCATAAAAGCGGTGACGGTGCGCAACGCGTGCCTGCGATTGAAGTGATGCTGCGTTCAC
CTTTGATCGTGGACTTGATTCGCAAGGGTGCCGTCGTCGAGATCAAGGACGTCATGAAGCGCTCTCAACAGCTGGGCATG
ATGACCTTCGATAAATCGCTATATGCGTTGCATCAGCAGGGCTTGATTACTGAAGAGGTTGCATTGGCACATGCAGATTC
TGCCAACGACTTGCGACTGATGATCAAGTTTGGCGATTCCGACAGTGCACAGGAGGCTCAGTTGGATGTCTTGAATGCGG
CGAGTCGTTTCTCGCTTCAAGACGACGATGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.334

98.177

0.612
  pilU Vibrio cholerae strain A1552

56.369

96.094

0.542
  pilU Acinetobacter baylyi ADP1

56.497

92.188

0.521
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.638

89.844

0.401
  pilT Pseudomonas aeruginosa PAK

41.791

87.24

0.365
  pilT Neisseria meningitidis 8013

40.29

89.844

0.362

Multiple sequence alignment