Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   E3Z27_RS02045 Genome accession   NZ_CP046874
Coordinates   464309..466381 (+) Length   690 a.a.
NCBI ID   WP_159284822.1    Uniprot ID   -
Organism   Pseudomonas mediterranea strain S58     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 459309..471381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3Z27_RS02025 (E3Z27_02030) pilM 461523..462587 (+) 1065 WP_047700492.1 pilus assembly protein PilM Machinery gene
  E3Z27_RS02030 (E3Z27_02035) - 462587..463153 (+) 567 WP_047700491.1 PilN domain-containing protein -
  E3Z27_RS02035 (E3Z27_02040) pilO 463150..463773 (+) 624 WP_047700490.1 type 4a pilus biogenesis protein PilO -
  E3Z27_RS02040 (E3Z27_02045) - 463788..464312 (+) 525 WP_392397492.1 pilus assembly protein PilP -
  E3Z27_RS02045 (E3Z27_02050) pilQ 464309..466381 (+) 2073 WP_159284822.1 type IV pilus secretin PilQ Machinery gene
  E3Z27_RS02050 (E3Z27_02055) aroK 466386..466904 (+) 519 WP_041020548.1 shikimate kinase AroK -
  E3Z27_RS02055 (E3Z27_02060) aroB 467053..468153 (+) 1101 WP_047700487.1 3-dehydroquinate synthase -
  E3Z27_RS02060 (E3Z27_02065) - 468164..469735 (+) 1572 WP_055127140.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74810.98 Da        Isoelectric Point: 5.2033

>NTDB_id=409063 E3Z27_RS02045 WP_159284822.1 464309..466381(+) (pilQ) [Pseudomonas mediterranea strain S58]
MNRIFTAFGVSLWMALMSPMVLGANLKTLDVAALPGDRVELKLAFDGPPPTPRGYTTEQPARIALDLPGVTNQLARKTQD
LGSGNARSATVVEAGDRTRLIIGLTQLVPYNARVEGNNLFVVVGKGAEPRTAAQAPRPAAAGVVSARSSAPVGRAIRGVD
FQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIEPGGTFD
YSTYQTDNKLTVSVRPMTVEDLRKRDTDRAAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPW
DQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKAD
ERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNQGNWNTSGVTNGS
STTIGTPGSTSTNQPFVDMGAAGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
ASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQEVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDLVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=409063 E3Z27_RS02045 WP_159284822.1 464309..466381(+) (pilQ) [Pseudomonas mediterranea strain S58]
ATGAACAGGATTTTTACCGCCTTCGGTGTTTCGCTATGGATGGCGCTGATGTCACCGATGGTGCTTGGGGCGAACCTGAA
GACGCTGGATGTCGCGGCTCTGCCGGGGGACCGAGTCGAGTTGAAGCTCGCTTTCGATGGGCCTCCACCCACGCCCCGGG
GCTATACCACCGAGCAACCGGCCCGGATCGCCCTGGACCTGCCAGGGGTGACCAACCAGTTGGCGCGCAAGACCCAGGAC
CTTGGCAGCGGCAATGCGCGCAGTGCCACAGTGGTCGAGGCGGGGGATCGCACCCGGTTGATCATCGGCCTGACCCAATT
GGTGCCGTATAACGCTCGGGTCGAAGGTAATAATCTGTTTGTGGTGGTCGGCAAGGGCGCGGAGCCCAGGACTGCCGCCC
AGGCCCCGCGCCCGGCAGCTGCCGGGGTGGTGTCTGCCAGGAGCAGCGCGCCGGTGGGCAGGGCCATCCGTGGCGTGGAC
TTCCAGCGCGGGACCCAGGGTGAAGGCAATGTAGTAATCGACCTGTCGGACCCGTCCATCGCGCCGGATATCCAGGAGCG
GGAAGGCAAGATCATCCTCAACTTCGCCAGGACCCAATTGCCTGAACCGCTGCGGGTGCGCCTGGACGTCAAGGACTTCG
CCACCCCGGTGCAGTTCGTCAACGCCAGCGCCGGGTCCGACCGGGCCACCATCAGCATCGAGCCCGGCGGTACCTTCGAT
TACTCGACCTACCAGACCGACAACAAACTGACCGTCAGCGTCCGGCCCATGACGGTCGAGGACTTGCGCAAGCGCGACAC
CGACCGTGCCGCCTACAACGGTGAAAAACTGTCCCTGAATTTCCAGGACATCGAAGTGCGCTCGGTCCTGCAACTGATTG
CCGATTTTACCAACCTGAACCTGGTGGCAAGCGACACGGTGCAGGGCGGTATCACCTTGCGGCTGCAGAATGTTCCATGG
GACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGGCTGGACAAGCGCAAGGTCGGCAATGTGTTGCTGGTCGCCCCCGC
CGATGAGATTGCGGCTCGCGAGCGCCAGGAACTGGAGTCCCAGAAGCAGATTGCCGACCTGGCGCCGCTGCGGCGAGAGC
TGTTGCAGGTCAACTATGCGAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAGGCGAAAGCCGAT
GAACGCGGCACGATCACGGTGGATGAGCGAACCAACAACATCATCGCCTACCAGACCCAGGACCGGCTCGACGAGTTGCG
CCGGATCGTGGCCCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCCCGGATCGTCGAGGCCAACGTCGATTACG
ACAAGAGCCTGGGCGTGCGTTGGGGCGGCTCGGTGCAGAACCAGGGCAACTGGAACACTTCGGGTGTGACCAACGGCAGT
TCCACCACCATCGGCACGCCGGGCAGCACCAGCACCAACCAGCCATTCGTCGACATGGGCGCGGCGGGCAATACCTCGGG
CATCGGCATTGCATTCATTACCGATAACGTACTGCTGGACCTGGAACTGACGGCCATGGAGAAAACCGGCAACGGGGAAA
TCGTCTCCCAGCCCAAGGTGGTGACGTCGGACAAGGAAACCGCAAAAATCCTCAAGGGCACGGAAATCCCCTACCAGGAA
GCCAGTTCCAGTGGGGCTACCTCGGTGTCGTTCAAGGAAGCCTCGTTGTCGCTGGAGGTGACCCCGCAGATCACGCCGGA
CAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAACCGGACTACCTGAACAAGGTGCAGGAGGTGCCACCGATCA
AGAAGAACGAAGTCAACGCCAAGGTGCTGGTCAATGACGGCGAGACCATTGTGATTGGCGGGGTTTTCTCAAATACTCAG
AGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATCTGGTTTCGGA
GAAAAAATCCGAGCTGCTGGTATTCCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(374-439)

Secretin and TonB N terminus short domain (STN)

(300-346)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(525-681)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.308

100

0.749
  comQ Acinetobacter baylyi ADP1

41.354

100

0.416
  pilQ Acinetobacter baumannii strain A118

38.378

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.378

100

0.412
  pilQ Legionella pneumophila strain Lp02

38.724

97.681

0.378
  pilQ Legionella pneumophila strain ERS1305867

38.599

97.246

0.375

Multiple sequence alignment