Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   E3Z27_RS02025 Genome accession   NZ_CP046874
Coordinates   461523..462587 (+) Length   354 a.a.
NCBI ID   WP_047700492.1    Uniprot ID   A0AAX2D4Z0
Organism   Pseudomonas mediterranea strain S58     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 456523..467587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3Z27_RS02015 (E3Z27_02020) - 457346..458614 (+) 1269 WP_047700494.1 malic enzyme-like NAD(P)-binding protein -
  E3Z27_RS02020 (E3Z27_02025) - 458871..461315 (-) 2445 WP_408003361.1 penicillin-binding protein 1A -
  E3Z27_RS02025 (E3Z27_02030) pilM 461523..462587 (+) 1065 WP_047700492.1 pilus assembly protein PilM Machinery gene
  E3Z27_RS02030 (E3Z27_02035) - 462587..463153 (+) 567 WP_047700491.1 PilN domain-containing protein -
  E3Z27_RS02035 (E3Z27_02040) pilO 463150..463773 (+) 624 WP_047700490.1 type 4a pilus biogenesis protein PilO -
  E3Z27_RS02040 (E3Z27_02045) - 463788..464312 (+) 525 WP_392397492.1 pilus assembly protein PilP -
  E3Z27_RS02045 (E3Z27_02050) pilQ 464309..466381 (+) 2073 WP_159284822.1 type IV pilus secretin PilQ Machinery gene
  E3Z27_RS02050 (E3Z27_02055) aroK 466386..466904 (+) 519 WP_041020548.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38184.75 Da        Isoelectric Point: 4.9112

>NTDB_id=409062 E3Z27_RS02025 WP_047700492.1 461523..462587(+) (pilM) [Pseudomonas mediterranea strain S58]
MLRLFNKKAHTLLGIDISSTSVKLLELSRQGDRYRVESYAVEPLPANAVIEKNIAELEGVGQALSRVLVKARTPSRSVAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLDEVAIDFEVLGVSPRNSERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FSNMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=409062 E3Z27_RS02025 WP_047700492.1 461523..462587(+) (pilM) [Pseudomonas mediterranea strain S58]
GTGCTACGACTCTTCAATAAAAAAGCCCATACGCTTCTGGGCATCGATATCAGCTCCACCTCGGTGAAGCTGCTCGAATT
GAGCCGCCAGGGCGACCGTTATCGTGTCGAGTCCTACGCGGTCGAACCGTTGCCGGCCAATGCCGTGATCGAGAAGAACA
TCGCCGAGCTCGAGGGGGTCGGCCAGGCGCTGTCCCGGGTCCTGGTCAAGGCCAGGACGCCCTCGCGCAGCGTGGCGGTG
GCCGTGGCGGGTTCGGCGGTGATTACCAAGACCATCGAGATGGACGCCGGGCTGTCCGACGACGAGATGGAGAACCAGCT
CAAGATCGAGGCGGACCAGTACATTCCCTATCCGCTGGATGAGGTGGCCATCGATTTCGAGGTGCTGGGGGTGTCGCCGC
GCAACAGCGAGCGGGTCGAGGTGCTGCTGGCGGCCTGTCGCAAGGAAAACGTCGAGGTGCGCGAGGCGGCGCTGGCATTG
GCGGGACTGACGGCCCGGGTGGTCGATGTGGAAGCCTACGCGTTGGAGCGCGCTTTCGGCCTGCTCGCCACGCAACTGGC
CGCATCCCAGGAGCGGCTGACCGTCGCGGTGATCGACATCGGCGCCACCATGACCACCCTGAGCGTGCTGCACAACGGGC
GCATCATCTATACCCGCGAGCAATTGTTCGGCGGTCGCCAGCTCACCGAGGAAATCCAGCGGCGCTACGGCCTGACCCTC
GAACAGGCCGGCCTGGCAAAGAAGCAGGGCGGCTTGCCGGACGACTACCTCAGCGAGGTGTTGCAGCCCTTTCGCGAAGC
GTTGGTGCAGCAGGTTTCGCGGTCCCTGCAGTTTTTCTTCGCTTCGGGCCAGTACAGTGCAGTGGACCACATCCTGTTGG
CTGGGGGCACGGCGTCTGTGGCCGGGCTGGACCGGCTGATCGAGCAGCGCCTGGGCACGCCGACCCAGGTGGCCAACCCG
TTTTCCAACATGGCCCTGGGCAGCAAGGTCAATGCCGGCGCCCTGGCCAGTGACGCGCCGGCGCTGATGATCGCCTGCGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.588

100

0.486

Multiple sequence alignment