Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DELCS14_RS05610 Genome accession   NC_015563
Coordinates   1271749..1273491 (-) Length   580 a.a.
NCBI ID   WP_013800820.1    Uniprot ID   -
Organism   Delftia sp. Cs1-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1266749..1278491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DELCS14_RS05580 (DelCs14_1116) - 1267328..1267846 (+) 519 WP_012207292.1 NUDIX domain-containing protein -
  DELCS14_RS05585 (DelCs14_1117) - 1267857..1268087 (-) 231 WP_085929076.1 DNA gyrase inhibitor YacG -
  DELCS14_RS05590 (DelCs14_1118) zapD 1268105..1268860 (-) 756 WP_012207290.1 cell division protein ZapD -
  DELCS14_RS05595 (DelCs14_1119) coaE 1268950..1269594 (-) 645 WP_013800817.1 dephospho-CoA kinase -
  DELCS14_RS05600 (DelCs14_1120) pilD 1269609..1270493 (-) 885 WP_013800818.1 A24 family peptidase Machinery gene
  DELCS14_RS05605 (DelCs14_1121) pilC 1270493..1271710 (-) 1218 WP_013800819.1 type II secretion system F family protein Machinery gene
  DELCS14_RS05610 (DelCs14_1122) pilB 1271749..1273491 (-) 1743 WP_013800820.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DELCS14_RS05620 (DelCs14_1123) - 1273732..1274661 (-) 930 WP_013800821.1 polyprenyl synthetase family protein -
  DELCS14_RS05625 (DelCs14_1124) rplU 1274892..1275203 (+) 312 WP_012207284.1 50S ribosomal protein L21 -
  DELCS14_RS05630 (DelCs14_1125) rpmA 1275219..1275476 (+) 258 WP_012207283.1 50S ribosomal protein L27 -
  DELCS14_RS05635 (DelCs14_1126) cgtA 1275626..1276741 (+) 1116 WP_012207282.1 Obg family GTPase CgtA -
  DELCS14_RS05640 (DelCs14_1127) proB 1276825..1277973 (+) 1149 WP_012207281.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62787.93 Da        Isoelectric Point: 5.3479

>NTDB_id=40823 DELCS14_RS05610 WP_013800820.1 1271749..1273491(-) (pilB) [Delftia sp. Cs1-4]
MAVADSSLKNASAVASLPGIGRALISAGKVSQQAAEAAASKAGANRTSFIAELSNAGILSALEVAETVSTMFSAPLLDLD
AINAARLPHELLDAKIISSYRVLPLSKRGNRITVATADPTQQEAAEQIKFTTQLGVDWIIVEADKLERLIAQQSKSASET
MESYSGGGDFEFGDLKPDEVAEEKDNTAATEVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIAS
PPIAIKEKLASRIKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KARLLEAIGRPYGMILVTGPTGSGKTVSLYTCLNMLNKAGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKEPAD
IPREALLEAGYDEADIDGSWVTYKPVGCQACNNGYKGRVGIYQVMPISEEIQRIILQDGSALEIAAQAKREGVRSLRGSG
LHKARLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=40823 DELCS14_RS05610 WP_013800820.1 1271749..1273491(-) (pilB) [Delftia sp. Cs1-4]
ATGGCTGTTGCTGACTCCTCGCTCAAGAATGCTTCCGCTGTTGCATCGCTTCCCGGTATCGGGCGAGCCCTGATATCAGC
AGGCAAGGTGTCGCAGCAGGCAGCGGAAGCCGCCGCCAGCAAGGCCGGCGCCAACCGCACCTCGTTCATTGCCGAGTTGA
GCAACGCAGGCATCCTCTCAGCCCTGGAAGTGGCCGAGACGGTCTCCACCATGTTCAGCGCGCCGCTGCTCGATCTTGAT
GCCATCAACGCCGCGCGCCTGCCGCATGAGTTGCTGGACGCCAAGATCATCAGCTCCTATCGCGTCCTCCCCTTGAGCAA
GCGAGGCAACCGCATCACGGTGGCCACGGCCGACCCGACACAGCAGGAAGCTGCAGAGCAGATCAAGTTCACCACCCAGC
TAGGCGTGGATTGGATCATCGTCGAAGCCGACAAGCTGGAACGCCTGATAGCTCAGCAGAGCAAAAGCGCCAGCGAAACC
ATGGAGAGCTACAGCGGAGGCGGCGATTTCGAGTTCGGCGACCTCAAGCCCGACGAGGTTGCCGAGGAAAAGGACAACAC
GGCGGCAACGGAAGTCGAGGACGCGCCCATCGTCAAGTTCCTGCACAAAATGCTGCTCGACGCCTTCAACATGCGCGCGT
CGGACCTGCATTTCGAGCCTTACGAACATCAGTACCGCGTACGCTTCCGGATTGACGGCGAGTTGCGTGAGATTGCATCG
CCCCCCATTGCGATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCGCGCATGGACATCTCGGAAAAGCGGGTTCC
GCAAGACGGGCGCATGAAGCTCAAGGTGGGTCCGGACCGCGTCATCGATTTTCGCGTGAGCACCCTGCCCACGCTCTTTG
GCGAGAAGATCGTGATCCGTATTCTGGACCCGAGCTCGGCCAAGATGGGCATTGATGCCCTGGGCTACGAACCCGAGGAA
AAGGCACGCCTGCTTGAAGCCATCGGGCGCCCCTACGGAATGATTCTGGTGACAGGCCCCACCGGCTCGGGCAAGACGGT
GTCGCTGTACACCTGCCTGAACATGCTGAACAAGGCCGGGGTGAACATCGCGACGGCAGAAGACCCCTCGGAAATCAACC
TGCCCGGCGTCAACCAAGTCAACGTGAACGAAAAGGCAGGCCTCACCTTTGCGACGGCGCTGAAGGCCTTCCTGCGCCAG
GATCCCGACATCATCATGGTCGGTGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCGCAGACCGGCCA
CTTGGTGCTGTCCACGCTGCACACCAACGACGCACCGACCACATTGACGCGCATGCGCAACATGGGCATTGCCCCCTTCA
ACATCGCATCGAGCGTGATCCTGATCACCGCCCAGCGGCTGGCCAGGCGCCTGTGCCCCCAGTGCAAGGAGCCTGCGGAT
ATCCCGCGGGAGGCGCTGCTGGAGGCGGGCTATGACGAGGCGGACATCGACGGTAGCTGGGTCACCTACAAGCCCGTGGG
CTGCCAGGCATGCAACAACGGCTACAAGGGACGCGTCGGCATCTACCAGGTGATGCCGATCAGCGAGGAAATCCAGCGCA
TCATCCTGCAAGACGGCAGCGCGCTGGAGATCGCGGCACAAGCCAAGCGTGAAGGCGTGCGGTCTCTGCGCGGATCCGGA
CTGCACAAGGCCAGACTAGGACTGACCTCACTCGAAGAAGTGCTGGCAGTTACCAATGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-153)

Type II/IV secretion system protein (T2SSE)

(203-470)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.712

98.103

0.547
  pilB Acinetobacter baumannii D1279779

56.028

97.241

0.545
  pilF Neisseria gonorrhoeae MS11

52.473

97.586

0.512
  pilB Legionella pneumophila strain ERS1305867

50.615

98.103

0.497
  pilB Vibrio cholerae strain A1552

46.461

95

0.441
  pilB Vibrio parahaemolyticus RIMD 2210633

45.554

95

0.433
  pilB Vibrio campbellii strain DS40M4

43.717

97.414

0.426
  pilF Thermus thermophilus HB27

38.204

97.931

0.374

Multiple sequence alignment