Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSEFU_RS18970 Genome accession   NC_015556
Coordinates   4091885..4093585 (+) Length   566 a.a.
NCBI ID   WP_013792870.1    Uniprot ID   F6AGH6
Organism   Pseudomonas fulva 12-X     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4081569..4091171 4091885..4093585 flank 714


Gene organization within MGE regions


Location: 4081569..4093585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSEFU_RS18920 (Psefu_3782) - 4081671..4082741 (+) 1071 WP_232285999.1 glycosyltransferase family 4 protein -
  PSEFU_RS18925 (Psefu_3783) - 4082816..4083694 (+) 879 WP_198136651.1 glycosyltransferase -
  PSEFU_RS18930 (Psefu_3784) - 4083691..4084734 (+) 1044 WP_013792864.1 class I SAM-dependent methyltransferase -
  PSEFU_RS22960 (Psefu_3785) - 4084739..4085668 (+) 930 WP_013792865.1 glycosyltransferase -
  PSEFU_RS18940 (Psefu_3786) - 4085594..4086859 (-) 1266 WP_013792866.1 lipopolysaccharide biosynthesis protein -
  PSEFU_RS18945 - 4086862..4087512 (-) 651 WP_049792729.1 ATP-binding protein -
  PSEFU_RS22590 - 4087593..4088000 (-) 408 WP_157139357.1 hypothetical protein -
  PSEFU_RS23690 - 4087942..4088211 (-) 270 WP_157139399.1 ATP-binding protein -
  PSEFU_RS18955 (Psefu_3788) - 4088365..4089216 (-) 852 WP_013792867.1 glycosyltransferase family 2 protein -
  PSEFU_RS18960 (Psefu_3789) - 4089213..4091171 (-) 1959 WP_013792868.1 tetratricopeptide repeat protein -
  PSEFU_RS22965 (Psefu_3790) pilA 4091196..4091612 (-) 417 WP_420042201.1 pilin Machinery gene
  PSEFU_RS18970 (Psefu_3791) pilB 4091885..4093585 (+) 1701 WP_013792870.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62463.35 Da        Isoelectric Point: 5.6115

>NTDB_id=40776 PSEFU_RS18970 WP_013792870.1 4091885..4093585(+) (pilB) [Pseudomonas fulva 12-X]
MSENIPLSGLAKQVVLAELLDEKAAQQAQVQAKRNKLSLVHYLAQNKLVKSRALAELTADQFGIAYFDLSALDKESQPKD
LVSEKLVRQHRALPLWRRGNKLFIGISDPSNHQVVTDIQFSTGLSTEALLVEDDKLGDAIEKFFDTGHTGLEDMGDVDLD
GLETESVDDDKKPEGGQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYERTYRVRFRTDGILHETARPPIQLAPRISARL
KVMAGLDISERRKPQDGRIKMKISKSKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLNALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTPDVNISTAEDPVEINLEGINQVNVNPKQGMDFTQALRAFLRQDPDIIMVGEIRDL
DTASIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCSSCKKEVDIPREALLEEGFPE
DKIGSFKIYGPVGCENCKGGYKGRVGIYEVVKNTPSLQRIIMEEGNSIDIAAQMRKDGFNDLRTSALVKAMQGVTSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=40776 PSEFU_RS18970 WP_013792870.1 4091885..4093585(+) (pilB) [Pseudomonas fulva 12-X]
ATGAGCGAGAACATTCCCCTTTCCGGCCTGGCCAAGCAGGTGGTGCTGGCTGAGCTGCTGGACGAGAAGGCCGCTCAGCA
AGCGCAGGTGCAAGCCAAACGCAACAAGCTCTCCCTTGTTCATTATCTGGCTCAGAACAAGCTGGTAAAAAGCCGCGCGC
TGGCGGAGCTGACGGCGGATCAGTTCGGCATCGCTTATTTCGACCTCAGCGCCCTAGATAAGGAGAGCCAACCAAAAGAT
CTAGTTAGTGAGAAGCTGGTGCGTCAGCACCGCGCCCTGCCCTTGTGGCGCCGCGGCAACAAGCTCTTTATCGGCATCTC
CGACCCCTCCAACCACCAGGTGGTTACCGATATTCAGTTCAGCACCGGCCTTTCTACGGAGGCGCTGCTGGTCGAAGACG
ACAAGCTAGGCGACGCCATCGAGAAGTTCTTCGATACTGGCCATACCGGCCTGGAAGACATGGGCGACGTTGATCTCGAT
GGCCTGGAAACCGAAAGCGTCGACGACGACAAGAAGCCAGAGGGTGGTCAGGATGCCGATGACGCTCCGGTAGTGCGCTT
CGTCAACAAGATGCTGCTCGATGCCATCAAAGGCGGCTCCTCCGATCTGCACTTCGAGCCTTACGAGCGTACCTATCGCG
TGCGCTTTCGTACAGACGGAATTTTACATGAGACGGCACGCCCGCCGATCCAGTTGGCACCGCGTATTTCTGCGCGTTTG
AAGGTAATGGCAGGCCTTGATATCTCCGAGCGGCGTAAGCCACAGGACGGCCGTATCAAGATGAAGATTTCCAAGAGCAA
ATCCATCGACTTTCGCGTTAACACCCTGCCGACGCTGTGGGGCGAAAAGATCGTGATGCGGATTCTCGATTCTTCCAGCG
CGCAGATGGGCATCGACGCCCTAGGTTACGAGGAAGACCAGAAGGAGCTGTACCTCAACGCCCTTAAACAGCCACAGGGC
ATGATTTTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTATCTCTGTATACCGGCCTCAATATTCTCAACACCCCGGA
CGTGAATATTTCCACGGCTGAAGACCCTGTGGAAATCAACCTAGAAGGCATCAACCAGGTCAACGTCAACCCCAAACAGG
GGATGGACTTTACCCAGGCACTGCGCGCCTTTCTGCGCCAGGACCCGGACATCATCATGGTCGGTGAGATTCGTGATCTG
GACACCGCTTCCATCGCCATCAAGGCCGCGCAGACAGGCCATATGGTGATGTCGACCCTGCATACCAATAGCGCTGCGGA
GACGCTAACTCGCCTACGTAATATGGGTGTGCCCTCGTTCAACATTGCCACCTCGGTCAACCTGATCATTGCTCAACGTC
TGGCGCGCAAGCTGTGTTCGTCCTGCAAAAAGGAAGTCGATATCCCGCGCGAAGCATTGCTGGAGGAAGGCTTTCCGGAG
GACAAGATAGGTAGCTTCAAGATCTACGGCCCGGTTGGCTGCGAAAACTGCAAAGGCGGCTATAAAGGTCGTGTCGGTAT
TTATGAAGTGGTTAAAAACACGCCAAGCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGATATTGCCGCGCAAA
TGCGCAAAGACGGCTTTAACGACCTGCGCACCTCGGCCCTTGTGAAGGCCATGCAAGGCGTGACCAGCCTTGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6AGH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.894

100

0.599
  pilB Acinetobacter baylyi ADP1

58.259

99.47

0.58
  pilB Legionella pneumophila strain ERS1305867

55.07

100

0.557
  pilB Vibrio cholerae strain A1552

49.636

97.173

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

50.743

95.053

0.482
  pilF Neisseria gonorrhoeae MS11

48.399

99.293

0.481
  pilB Vibrio campbellii strain DS40M4

49.358

96.29

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

36.408

100

0.398
  pilF Thermus thermophilus HB27

36.926

100

0.369

Multiple sequence alignment