Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GPY25_RS16220 Genome accession   NZ_CP046779
Coordinates   3248725..3249948 (+) Length   407 a.a.
NCBI ID   WP_005493981.1    Uniprot ID   A0A249W378
Organism   Vibrio parahaemolyticus strain 2014V-1066     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3243725..3254948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY25_RS16200 ampD 3244767..3245318 (-) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY25_RS16205 nadC 3245411..3246298 (+) 888 WP_005493988.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY25_RS16210 - 3246562..3247014 (+) 453 WP_005493986.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY25_RS16215 pilB 3247016..3248701 (+) 1686 WP_005493983.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY25_RS16220 pilC 3248725..3249948 (+) 1224 WP_005493981.1 type II secretion system F family protein Machinery gene
  GPY25_RS16225 pilD 3250013..3250882 (+) 870 WP_005493974.1 A24 family peptidase Machinery gene
  GPY25_RS16230 coaE 3250883..3251497 (+) 615 WP_005493969.1 dephospho-CoA kinase -
  GPY25_RS16235 zapD 3251525..3252265 (+) 741 WP_005480890.1 cell division protein ZapD -
  GPY25_RS16240 yacG 3252334..3252528 (+) 195 WP_005462546.1 DNA gyrase inhibitor YacG -
  GPY25_RS16245 rplS 3252944..3253297 (-) 354 WP_005462554.1 50S ribosomal protein L19 -
  GPY25_RS16250 trmD 3253339..3254082 (-) 744 WP_005462562.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  GPY25_RS16255 rimM 3254110..3254658 (-) 549 WP_005462552.1 ribosome maturation factor RimM -
  GPY25_RS16260 rpsP 3254687..3254935 (-) 249 WP_005379962.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45208.33 Da        Isoelectric Point: 10.4368

>NTDB_id=407614 GPY25_RS16220 WP_005493981.1 3248725..3249948(+) (pilC) [Vibrio parahaemolyticus strain 2014V-1066]
MKTTTPQLKNFRWKGVNSSGKKTSGQTLAMSETEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGKDITVFTRQISTML
VTGVPLVQALKLVSDNHKKAEMKSILMSVTRAVEAGTPMSKAMRTASNHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVILVALGVSFIMLTKVIPEFEKMFVGFGAELPWFTRQVLDLSAWTQNWSPFIALGSISVFISA
RVLSKRSDSFRLMLNRSVLKFPVLGAVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=407614 GPY25_RS16220 WP_005493981.1 3248725..3249948(+) (pilC) [Vibrio parahaemolyticus strain 2014V-1066]
ATGAAAACTACGACACCACAATTAAAGAACTTCCGTTGGAAAGGCGTCAACAGCTCAGGGAAAAAGACCTCTGGTCAAAC
TCTGGCAATGAGCGAAACCGAAGTACGAGAGCGCTTAGATGCCCAGCACATCAAAATCAAAAAGCTAAAGAAAAGCAGCA
TCTCTTTTTTGACCAAACTTAGCCATCGCGTAAAAGGAAAAGACATCACGGTTTTCACTCGTCAAATCTCAACGATGCTG
GTAACCGGTGTGCCGCTAGTACAAGCATTGAAATTGGTCTCTGATAACCACAAAAAGGCGGAAATGAAATCCATTTTGAT
GAGTGTAACTCGGGCTGTTGAAGCGGGTACGCCGATGTCCAAAGCGATGCGTACCGCCAGTAACCATTTTGACCCGCTGT
ATACCGACCTGATTGCGACAGGAGAACAATCGGGCAACTTAGCCGAAGTGTTCGAACGCTTAGCTACCTACAGGGAAAAA
AATGAGCAACTTAGAGCCAAAGTGATCAAGGCTCTAATATATCCAGCCATGGTAATTCTAGTTGCACTGGGAGTATCGTT
TATCATGCTAACCAAGGTCATTCCCGAGTTTGAAAAAATGTTTGTTGGCTTTGGCGCTGAATTGCCATGGTTTACAAGGC
AAGTCTTAGATCTTTCCGCTTGGACACAAAACTGGAGCCCATTTATCGCACTTGGCTCCATCAGTGTATTTATCTCCGCG
AGAGTACTCTCTAAGCGTTCAGATTCTTTTCGTTTAATGCTCAACCGATCTGTACTTAAGTTTCCAGTCCTTGGAGCGGT
TTTATCGAAAGCCGCTATCGCAAAATTTAGTCGAACACTCGCTACGAGCTTTACGGCGGGCATTCCGATTCTAACCAGTT
TAAAAACCACATCGAAAACGTCCGGAAATATGCATTATCAATTGGCTATCGAAGAGGTTTATCGAGATACCGCAGCAGGA
ATGCCGATGTATGTTGCAATGCGCAATTGCAACGTGTTCCCTGAGTTGGTGCTGCAAATGGTTATGATCGGCGAAGAATC
CGGCCGACTTGACGACATGCTCAATAAAGTCGCCACAATTTACGAGTTTGAAGTGGACAACACCGTCGATAACCTCAGTA
AAATCTTAGAGCCATTGATTATCGTTTTTTTAGGTATCGTTGTCGGTGGCTTGGTCACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249W378

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

85.749

100

0.857
  pilC Vibrio cholerae strain A1552

74.074

99.509

0.737
  pilC Acinetobacter baylyi ADP1

43

98.28

0.423
  pilC Acinetobacter baumannii D1279779

41.439

99.017

0.41
  pilG Neisseria gonorrhoeae MS11

40.494

99.509

0.403
  pilG Neisseria meningitidis 44/76-A

40.247

99.509

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398
  pilC Legionella pneumophila strain ERS1305867

39.803

100

0.398

Multiple sequence alignment