Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GPY34_RS20170 Genome accession   NZ_CP046778
Coordinates   2309790..2311013 (-) Length   407 a.a.
NCBI ID   WP_005493981.1    Uniprot ID   A0A249W378
Organism   Vibrio parahaemolyticus strain 2014V-1125     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2304790..2316013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY34_RS20130 rpsP 2304803..2305051 (+) 249 WP_005379962.1 30S ribosomal protein S16 -
  GPY34_RS20135 rimM 2305080..2305628 (+) 549 WP_005462552.1 ribosome maturation factor RimM -
  GPY34_RS20140 trmD 2305656..2306399 (+) 744 WP_005462562.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  GPY34_RS20145 rplS 2306441..2306794 (+) 354 WP_005462554.1 50S ribosomal protein L19 -
  GPY34_RS20150 yacG 2307210..2307404 (-) 195 WP_005462546.1 DNA gyrase inhibitor YacG -
  GPY34_RS20155 zapD 2307473..2308213 (-) 741 WP_005480890.1 cell division protein ZapD -
  GPY34_RS20160 coaE 2308241..2308855 (-) 615 WP_005493969.1 dephospho-CoA kinase -
  GPY34_RS20165 pilD 2308856..2309725 (-) 870 WP_005493974.1 A24 family peptidase Machinery gene
  GPY34_RS20170 pilC 2309790..2311013 (-) 1224 WP_005493981.1 type II secretion system F family protein Machinery gene
  GPY34_RS20175 pilB 2311037..2312722 (-) 1686 WP_005493983.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY34_RS20180 - 2312724..2313176 (-) 453 WP_005493986.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY34_RS20185 nadC 2313440..2314327 (-) 888 WP_005493988.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY34_RS20190 ampD 2314420..2314971 (+) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45208.33 Da        Isoelectric Point: 10.4368

>NTDB_id=407574 GPY34_RS20170 WP_005493981.1 2309790..2311013(-) (pilC) [Vibrio parahaemolyticus strain 2014V-1125]
MKTTTPQLKNFRWKGVNSSGKKTSGQTLAMSETEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGKDITVFTRQISTML
VTGVPLVQALKLVSDNHKKAEMKSILMSVTRAVEAGTPMSKAMRTASNHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVILVALGVSFIMLTKVIPEFEKMFVGFGAELPWFTRQVLDLSAWTQNWSPFIALGSISVFISA
RVLSKRSDSFRLMLNRSVLKFPVLGAVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=407574 GPY34_RS20170 WP_005493981.1 2309790..2311013(-) (pilC) [Vibrio parahaemolyticus strain 2014V-1125]
ATGAAAACTACGACACCACAATTAAAGAACTTCCGTTGGAAAGGCGTCAACAGCTCAGGGAAAAAGACCTCTGGTCAAAC
TCTGGCAATGAGCGAAACCGAAGTACGAGAGCGCTTAGATGCCCAGCACATCAAAATCAAAAAGCTAAAGAAAAGCAGCA
TCTCTTTTTTGACCAAACTTAGCCATCGCGTAAAAGGAAAAGACATCACGGTTTTCACTCGTCAAATCTCAACGATGCTG
GTAACCGGTGTGCCGCTAGTACAAGCATTGAAATTGGTCTCTGATAACCACAAAAAGGCGGAAATGAAATCCATTTTGAT
GAGTGTAACTCGGGCTGTTGAAGCGGGTACGCCGATGTCCAAAGCGATGCGTACCGCCAGTAACCATTTTGACCCGCTGT
ATACCGACCTGATTGCGACAGGAGAACAATCGGGCAACTTAGCCGAAGTGTTCGAACGCTTAGCTACCTACAGGGAAAAA
AATGAGCAACTTAGAGCCAAAGTGATCAAGGCTCTAATATATCCAGCCATGGTAATTCTAGTTGCACTGGGAGTATCGTT
TATCATGCTAACCAAGGTCATTCCCGAGTTTGAAAAAATGTTTGTTGGCTTTGGCGCTGAATTGCCATGGTTTACAAGGC
AAGTCTTAGATCTTTCCGCTTGGACACAAAACTGGAGCCCATTTATCGCACTTGGCTCCATCAGTGTATTTATCTCCGCG
AGAGTACTCTCTAAGCGTTCAGATTCTTTTCGTTTAATGCTCAACCGATCTGTACTTAAGTTTCCAGTCCTTGGAGCGGT
TTTATCGAAAGCCGCTATCGCAAAATTTAGTCGAACACTCGCTACGAGCTTTACGGCGGGCATTCCGATTCTAACCAGTT
TAAAAACCACATCGAAAACGTCCGGAAATATGCATTATCAATTGGCTATCGAAGAGGTTTATCGAGATACCGCAGCAGGA
ATGCCGATGTATGTTGCAATGCGCAATTGCAACGTGTTCCCTGAGTTGGTGCTGCAAATGGTTATGATCGGCGAAGAATC
CGGCCGACTTGACGACATGCTCAATAAAGTCGCCACAATTTACGAGTTTGAAGTGGACAACACCGTCGATAACCTCAGTA
AAATCTTAGAGCCATTGATTATCGTTTTTTTAGGTATCGTTGTCGGTGGCTTGGTCACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249W378

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

85.749

100

0.857
  pilC Vibrio cholerae strain A1552

74.074

99.509

0.737
  pilC Acinetobacter baylyi ADP1

43

98.28

0.423
  pilC Acinetobacter baumannii D1279779

41.439

99.017

0.41
  pilG Neisseria gonorrhoeae MS11

40.494

99.509

0.403
  pilG Neisseria meningitidis 44/76-A

40.247

99.509

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398
  pilC Legionella pneumophila strain ERS1305867

39.803

100

0.398

Multiple sequence alignment