Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPY20_RS12695 Genome accession   NZ_CP046756
Coordinates   1202917..1204605 (-) Length   562 a.a.
NCBI ID   WP_158129991.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2015AW-0077     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1197917..1209605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY20_RS12670 yacG 1199098..1199295 (-) 198 WP_020332195.1 DNA gyrase inhibitor YacG -
  GPY20_RS12675 zapD 1199350..1200090 (-) 741 WP_158129988.1 cell division protein ZapD -
  GPY20_RS12680 coaE 1200123..1200731 (-) 609 WP_158129989.1 dephospho-CoA kinase -
  GPY20_RS12685 pilD 1200734..1201603 (-) 870 WP_024374403.1 A24 family peptidase Machinery gene
  GPY20_RS12690 pilC 1201655..1202881 (-) 1227 WP_158129990.1 type II secretion system F family protein Machinery gene
  GPY20_RS12695 pilB 1202917..1204605 (-) 1689 WP_158129991.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY20_RS12700 - 1204619..1205035 (-) 417 WP_158129992.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY20_RS12705 nadC 1205267..1206154 (-) 888 WP_158129993.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY20_RS12710 ampD 1206307..1206858 (+) 552 WP_158129994.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY20_RS12715 fldB 1206863..1207384 (-) 522 WP_020430452.1 flavodoxin FldB -
  GPY20_RS12720 xerD 1207525..1208433 (+) 909 WP_020331983.1 site-specific tyrosine recombinase XerD -
  GPY20_RS12725 dsbC 1208450..1209214 (+) 765 WP_020331984.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 61997.85 Da        Isoelectric Point: 6.2642

>NTDB_id=407245 GPY20_RS12695 WP_158129991.1 1202917..1204605(-) (pilB) [Vibrio fluvialis strain 2015AW-0077]
MLTNLLAILRQADLLSPTQEQAVMDHIHASGTNVPEALIDLAIFHHHDLTEHLSQIFGLPNTDLTRFDFAPLCQQLGLRE
LITRYQALPLNSNGQILTLAVSDPTHLQVEDEFRFATGLQVELVLADHKALQAAIRRLYGRAITSHGTQGKEISQDELAS
LVQVSDDEKTSIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESFYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLNADTAIDMRVSTLPTLWGEKIVLRLLDSSASSLDIDKLGYNDAQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTDEVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAESVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVATSPSPTLQQQFHLSSQQ
PIYAANPSGCNECTNGYSGRVGIYEVMAFNSALAEAIMQRASIHQIESIAQQHGMQTLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=407245 GPY20_RS12695 WP_158129991.1 1202917..1204605(-) (pilB) [Vibrio fluvialis strain 2015AW-0077]
ATGCTCACCAACCTACTCGCCATCCTGCGTCAGGCCGATTTGCTTAGCCCTACGCAGGAACAGGCCGTGATGGATCATAT
TCACGCTTCCGGAACCAATGTGCCGGAGGCGTTGATCGATTTGGCGATATTCCATCACCACGATCTGACCGAGCATCTAA
GCCAGATCTTTGGTCTGCCGAATACGGATCTGACCCGTTTTGACTTCGCGCCGCTTTGCCAGCAGTTGGGGCTGCGCGAG
CTCATCACCCGTTATCAGGCTCTGCCCCTCAACAGCAACGGTCAGATACTGACTCTTGCGGTATCCGATCCGACTCACCT
ACAAGTTGAAGACGAGTTTCGTTTTGCGACCGGGTTACAAGTAGAACTGGTATTAGCTGATCATAAAGCACTGCAAGCCG
CTATTCGCCGCTTGTATGGCCGAGCCATCACGTCACATGGCACGCAGGGCAAGGAGATCAGCCAGGACGAACTGGCCAGT
CTGGTGCAGGTTTCCGATGACGAAAAAACATCAATCGAAGATCTGAGTCAGGATGACTCCCCGGTCAGTCGTTTCATCAA
CCAAATCCTGCTTGACGCGGTGCGTAAAGGCGCTTCTGATATCCATTTTGAGCCCTATGAATCCTTTTATCGCATTCGCC
TGCGCTGCGACGGCATTTTGGTGGAAACCCAGCAACCAGCCAGCCATTTAAGCCGCCGACTGGCTGCGCGGATTAAGATC
TTATCGAAACTCGATATCGCTGAGCGACGTTTACCGCAAGACGGCCGCATCAAATTACGCCTCAATGCGGACACGGCCAT
TGATATGCGGGTCTCCACTCTGCCAACCTTGTGGGGGGAGAAAATCGTCCTGCGTCTGCTCGACAGCAGCGCGTCCAGCC
TCGATATCGACAAGTTGGGTTATAACGATGCGCAAAAGCAGCTTTATCTGGCGGCCCTCACCAAGCCGCAAGGCATGATT
CTGATGACTGGCCCGACGGGCAGCGGTAAGACAGTCTCACTCTATACCGGCCTTCGCATTCTCAACACCGACGAAGTGAA
TATTTCTACCGCCGAAGACCCGGTGGAAATTAACCTGCCCGGCATTAACCAGGTTCAGGTGCAGCCCAAAATTGGTTTTG
GCTTTGCGCAGGCTCTGCGCTCCTTTCTGCGTCAGGATCCCGATGTGGTAATGGTGGGCGAAATCCGCGATTTGGAAACG
GCAGAGATTGCCGTCAAAGCCGCCCAAACCGGCCACTTAGTGCTATCCACACTACACACCAACTCCGCCGCCGAAAGCGT
GATTCGGCTGGCGAACATGGGCATTGAACCGTTCAACCTCGCTTCATCGCTGAGCCTCATCATCGCTCAACGACTTGCCC
GGCGCCTTTGCCCTCATTGCAAAGTCGCCACATCGCCCTCTCCGACACTGCAGCAACAATTTCACTTGAGTTCTCAGCAG
CCTATCTATGCCGCCAACCCATCAGGCTGCAACGAGTGCACCAACGGTTACAGCGGGCGGGTGGGGATTTATGAAGTGAT
GGCATTTAACTCCGCGCTGGCTGAAGCGATTATGCAACGAGCCAGCATCCATCAAATCGAATCCATTGCCCAGCAACATG
GCATGCAAACGCTGTTGCAATCGGGGATCGAAAAACTTCGCGCCGGGATCACCAGTTTCAGTGAGTTGCAGCGTGTTCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.918

100

0.829
  pilB Vibrio campbellii strain DS40M4

72.42

100

0.724
  pilB Vibrio parahaemolyticus RIMD 2210633

71.886

100

0.719
  pilB Legionella pneumophila strain ERS1305867

52.611

91.993

0.484
  pilB Acinetobacter baumannii D1279779

50.096

92.349

0.463
  pilB Acinetobacter baylyi ADP1

48.387

93.772

0.454
  pilF Neisseria gonorrhoeae MS11

44.681

100

0.448
  pilF Thermus thermophilus HB27

37.544

100

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

37.636

97.865

0.368

Multiple sequence alignment